Wound Healing Assay

  • More consistent results well-to-well compared to homemade scratch assays
  • Useful for measuring cell migration, cell proliferation and wound closure
  • Inert inserts leave no residues that could impede proliferation or migration of cells

 

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CytoSelect™ 24-Well Wound Healing Assay
Catalog Number
CBA-120-5
Size
5 x 24 assays
Detection
Light Microscopy
Manual/Data Sheet Download
SDS Download
Price
$2,185.00
CytoSelect™ 24-Well Wound Healing Assay
Catalog Number
CBA-120
Size
24 assays
Detection
Light Microscopy
Manual/Data Sheet Download
SDS Download
Price
$525.00
CytoSelect™ 24-Well Wound Healing Assay, Trial Size
Catalog Number
CBA-120-T
Size
6 assays
Detection
Light Microscopy
Manual/Data Sheet Download
SDS Download
Price
$265.00
Product Details

Traditionally scratch assays have been used to study cell migration, cell proliferation and wound healing. However, these assays lack a consistently defined wound gap and can result in high inter-sample variation.

Our CytoSelect™ 24-Well Wound Healing Assay provides a much more consistent method to measure cell migration across a wound field gap in vitro. Proprietary inserts generate a consistent 0.9mm wound gap between the cells. Cells may then be treated or monitored for proliferation or migration across the wound field by imaging samples at fixed time points or by time-lapse microscopy.

Assay Principle.

Percent Closure of MEF/STO Cells. STO cells were tested using the CytoSelect™ 24-Well Wound Healing Assay. Cells were cultured 24 hours until a monolayer formed, at which time the inserts were removed to begin the assay. Cells were monitored under DAPI labeling and cell staining for determining percent closure.

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