24-Well Anoikis Assay

24-Well Anoikis Assay
  • Detect live and dead cells by microscopy, fluorescence, or flow cytometry
  • Quantify live cells on a standard microplate reader or fluorometer
  • Precoated plate included

 

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CytoSelect™ 24-Well Anoikis Assay
Catalog Number
CBA-080
Size
24 assays
Detection
Colorimetric / Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$465.00
Product Details

Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis.

Our CytoSelect™ Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.

Anoikis of Human Fibroblast BJ-TERT Cells. 50,000 cells/well were seeded in a control plate and cultured for 24 hours. Top: Cells on the control plate were stained with Calcein AM. Bottom: Cells on the Poly-HEMA coated plate were stained with EthD-1.

Recent Product Citations
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  3. Tan, Y. et al. (2018). Adipocytes fuel gastric cancer omental metastasis via PITPNC1-mediated fatty acid metabolic reprogramming. Theranostics. 8(19):5452-5468. doi: 10.7150/thno.28219.
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  6. Chen, H.Y. et al. (2018). Integrin alpha5beta1 suppresses rBMSCs anoikis and promotes nitric oxide production. Biomed Pharmacother. 99:1-8. doi: 10.1016/j.biopha.2018.01.038.
  7. Fu, X.T. et al. (2018). MicroRNA-30a suppresses autophagy-mediated anoikis resistance and metastasis in hepatocellular carcinoma. Cancer Lett. 412:108-117. doi: 10.1016/j.canlet.2017.10.012.
  8. Yu, M. et al (2017). Interference with Tim-3 protein expression attenuates the invasion of clear cell renal cell carcinoma and aggravates anoikis. Mol Med Rep. doi: 10.3892/mmr.2017.6136.
  9. Lu, S. et al. (2016). Expression of α-fetoprotein in gastric cancer AGS cells contributes to invasion and metastasis by influencing anoikis sensitivity. Oncol Rep. 35:2984-2990.
  10. Lee, H.W. et al. (2013). Tpl2 Kinase Impacts Tumor Growth and Metastasis of Clear Cell Renal Cell Carcinoma. Mol Cancer Res. 11:1375-1386.
  11. Sisto, M. et al. (2009). Fibulin-6 Expression and Anoikis in Human Salivary Gland Epithelial Cells: Implications in Sjogren's Syndrome. Int. Immunol. 21:303-311.
  12. Liu, H. et al. (2008). Cysteine-Rich Protein 61 and Connective Tissue Growth Factor Induce De-Adhesion and Anoikis of Retinal Pericytes. Endocrinology 149:1666-1677.