96-Well Cell Transformation Assays, Soft Agar with Cell Recovery

  • Proprietary modified soft agar medium
  • Fully quantify cell transformation with no manual cell counting
  • Results in 7-8 days, not 3 weeks 
  • Recover cells from soft agar medium for further downstream analysis

 

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General FAQs about Cell Transformation Assays

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CytoSelect™ 96-Well Cell Transformation Assay, Cell Recovery Compatible
Catalog Number
CBA-135
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$665.00
CytoSelect™ 96-Well Cell Transformation Assay, Cell Recovery Compatible
Catalog Number
CBA-135-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$2,850.00
CytoSelect™ 96-Well Cell Transformation Assay, Cell Recovery Compatible
Catalog Number
CBA-140
Size
96 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$695.00
CytoSelect™ 96-Well Cell Transformation Assay, Cell Recovery Compatible
Catalog Number
CBA-140-5
Size
5 x 96 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$2,960.00
CytoSelect™ 96-Well Cell Transformation Assay, Cell Recovery Compatible, Trial Size
Catalog Number
CBA-135-T
Size
24 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$330.00
CytoSelect™ 96-Well Cell Transformation Assay, Cell Recovery Compatible, Trial Size
Catalog Number
CBA-140-T
Size
24 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$350.00
Product Details

CytoSelect™ 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

These cell transformation assays are designed and optimized for 96-well plates, but can easily be adapted for use in 48, 24, 12 or 6-well plates. Both colorimetric and fluorometric formats are available.

CytoSelect™ 96-Well Cell Transformation Assay Principle.

Viability of Recovered Cells. HeLa and 293 cells were cultured for 6 days according to the assay protocol. Cells were recovered and the cell viability was determined by trypan blue exclusion.

Recent Product Citations
  1. Lim, S.K. et al. (2016). Wnt signaling promotes breast cancer by blocking ITCH-mediated degradation of YAP/TZA transcriptional coactivator WBP2. Cancer Res. 76:6278-6289.
  2. Kumar, A. et al. (2016). Tumor control by human cytomegalovirus in a murine model of hepatocellular carcinoma. Mol Ther. doi:10.1038/mto.2016.12 (#CBA-135).
  3. Mardin, B. R. et al. (2015). A cell-based model system links chromothripsis with hyperploidyMol Syst Biol. 11:828 (#CBA-135).
  4. Monot, M. et al. (2015). Early steps of Jaagsiekte sheep retrovirus-mediated cell transformation involve the interaction between env and the RALBP1 cellular protein. J Virol. 89:8462-8473 (#CBA-135).
  5. Bon, H. et al. (2015). Salt-inducible kinase 2 regulates mitotic progression and transcription in prostate cancer. Mol Cancer Res. 13:620-635 (#CBA-135).
  6. Fatemi, M. et al. (2014). Epigenetic silencing of CHD5, a novel tumor-suppressor gene, occurs in early colorectal cancer stages. Cancer. 120:172-180 (#CBA-135).
  7. Park, H. et al. (2014). Distinct roles of DKK1 and DKK2 in tumor angiogenesisAngiogenesis. 17:221-234 (#CBA-135).
  8. Wang, X. et al. (2014).  Commensal Bacteria Drive Endogenous Transformation and Tumour Stem Cell Marker Expression Through a Bystander Effect. Gut. 10.1136/gutjnl-2014-307213 (#CBA-135).
  9. Bottero, V. et al. (2013). Kaposi's Sarcoma-Associated Herpesvirus-Positive Primary Effusion Lymphoma Tumor Formation in NOD/SCID Mice Is Inhibited by Neomycin and Neamine Blocking Angiogenin's Nuclear Translocation. J. Virol. 87:11806-11820 (#CBA-135).
  10. Singh, R. et al. (2013). Increasing the Complexity of Chromatin: Functionally Distinct Roles for Replication-Dependent Histone H2A Isoforms in Cell Proliferation and Carcinogenesis. Nucleic Acids Res. 10.1093/nar/gkt736 (#CBA-135).
  11. Shukla, A. et al. (2013). Extracellular Signal–Regulated Kinase 5: A Potential Therapeutic Target for Malignant Mesotheliomas. Clin. Cancer Res. 19:2071-2083 (#CBA-135).
  12. Niccoli, S. et al. (2012).The Asian-American E6 Variant Protein of Human Papillomavirus 16 Alone Is Sufficient To Promote Immortalization, Transformation, and Migration of Primary Human Foreskin Keratinocytes. J. Virol. 86:12384-12396 (#CBA-135).
  13. Hong, S.W. et al. (2012). Ring Finger Protein 149 Is an E3 Ubiquitin Ligase Active on Wild-type v-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF). J. Biol. Chem. 287:24017-24025 (#CBA-135).
  14. Lee, H.J. et al. (2012). Chemokine (C-X-C Motif) Ligand 12 Is Associated with Gallbladder Carcinoma Progression and Is a Novel Independent Poor Prognostic Factor. Clin. Cancer. Res. 18:3270-3280 (#CBA-135). 
  15. Chapeau, E.A. et al.(2012).Ecotropic Viral Integration Site 1 (EVI1) Regulates Multiple Cellular Processes Important for Cancer and is a Synergistic Partner for FOS Protein in Invasive Tumors. Proc Natl Acad Sci 109:2168-2173.(#CBA-135)
  16. Lim, S.K. et al. (2011). Tyrosine Phosphorylation of Transcriptional Coactivator WW-Domain Binding Protein 2 Regulates Estrogen Receptor alpha Function in Breast Cancer via the Wnt Pathway. FASEB J. 25:3004-3018. (#CBA-135)
  17. Mathew, B. et al. (2011). The Novel Role of the Mu Opioid Receptor in Lung Cancer Progression: A Laboratory Investigation. Anesth. Analg. 112:558-567 (#CBA-135).
  18. Hirata, H. et al. (2010). Role of secreted Frizzled-related protein3 in Human Renal Cell Carcinoma. Cancer Res. 70:1896-1905 (#CBA-135).
  19. Xie, G. et al. (2009). Acetylcholine-Induced Activation of M3 Muscarinic Receptors Stimulates Robust Matrix Metalloproteinase Gene Expression in Human Colon Cancer Cells. Am. J. Physiol. Gastrointest. Liver Physiol. 296:G755-G763 (#CBA-135).
  20. Hirata, H. et al. (2009). Wnt Antagonist Gene DKK2 is Epigenetically Silenced and Inhibits Renal Cancer Progression through Apoptotic and Cell Cycle Pathways. Clin. Cancer Res. 15:5678-5687 (#CBA-135).
  21. Ke, X-S. et al. (2008). Epithelial to Mesenchymal Transition of a Primary Prostate Cell Line With Switches of Cell Adhesion Modules but Without Malignant Transformation. PLoS One 3(10):e3368 (#CBA-135).
  22. Montalbano, M. et al. (2016). Modeling of hepatocytes proliferation isolated from proximal and distal zones from human hepatocellular carcinoma lesion. PLoS One 11:e0153613 (#CBA-140).