Our CytoSelect™ 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.
Cells incubated using this assay may not be recovered intact. For recovery of intact viable cells, consider our Soft Agar Assay with Cell Recovery.
CytoSelect™ 96-Well Cell Transformation Assay Principle.
Anchorage-Independent Growth of HeLa Cells. HeLa cells were seeded at various concentrations and cultured for 6 days. HeLa cell transformation was determined according to the assay protocol.