Total Bile Acid Assays

Total Bile Acid Assay Kit
  • Measures total bile acid content as low as 1 µM (colorimetric) or 0.4 µM (fluorometric)
  • Suitable for plasma, serum, and cell or tissue lysates
  • Available with colorimetric or fluorometric detection
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Total Bile Acid Assay Kit (Colorimetric)
Catalog Number
100 assays
Manual/Data Sheet Download
SDS Download
Total Bile Acid Assay Kit (Fluorometric)
Catalog Number
100 assays
Manual/Data Sheet Download
SDS Download
Product Details

While bile acid synthesis is critical for the removal of cholesterol from the body, bile acids are also required for proper uptake of nutrients in the small intestine. Our Total Bile Acid Assay Kit provides a convenient 96-well plate-based method to measure the total bile acid content in a variety of sample types.

These assays are based on an enzyme driven reaction in which bile acids are incubated in the presence of 3-alpha hydroxysteroiddehydrogenase. The reaction used with the colorimetric kit requires the presence of NADH, and thio-NAD+. The thio-NAD+ is reduced to thio-NADH which is detected by colorimetric absorbance. The fluorometric kit requires incubation with NAD+, which is converted to NADH. Diaphorase then uses NADH to reduce resazurin to resorufin, which is detected fluormetrically at 560nm excitation and 590nm emission.

Recent Product Citations
  1. Choi, J.H. et al. (2020). Microfluidic confinement enhances phenotype and function of hepatocyte spheroids. Am J Physiol Cell Physiol. doi: 10.1152/ajpcell.00094.2020 (#MET-5005).
  2. Chen, P.B. et al. (2020). Directed remodeling of the mouse gut microbiome inhibits the development of atherosclerosis. Nat Biotechnol. doi: 10.1038/s41587-020-0549-5 (#MET-5005).
  3. Kennedy, L. et al. (2020). Biliary damage and liver fibrosis are ameliorated in a novel mouse model lacking l-histidine decarboxylase/histamine signaling. Lab Invest. doi: 10.1038/s41374-020-0405-8 (#STA-631).
  4. Feng, X. et al. (2020). Depletion of hepatic forkhead box O1 does not affect cholelithiasis in male and female mice. J Biol Chem. pii: jbc.RA119.012272. doi: 10.1074/jbc.RA119.012272 (#STA-631).
  5. Dao Thi, V.L. et al. (2020). Stem cell-derived polarized hepatocytes. Nat Commun. 11(1):1677. doi: 10.1038/s41467-020-15337-2 (#STA-631).
  6. Kumazaki, S. et al. (2019). Bile Acid Metabolism is an Intermediary Factor between Non-Alcoholic Steatohepatitis and Ischemic Heart Disease in SHRSP5/Dmcr Rats. J Nutri Food Sci. 9:763. doi: 10.35248/2155-9600.19.9.763 (#STA-631).
  7. Dächert, C. et al. (2019). Gene Expression Profiling of Different Huh7 Variants Reveals Novel Hepatitis C Virus Host Factors. Viruses. 12(1). pii: E36. doi: 10.3390/v12010036 (#STA-631).
  8. Siemienowicz, K.J. et al. (2019). Fetal androgen exposure is a determinant of adult male metabolic health. Sci Rep. 9(1):20195. doi: 10.1038/s41598-019-56790-4 (#STA-631).
  9. Torres, S.E. et al. (2019). Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether. Elife. doi: 10.7554/eLife.46595 (#STA-631).
  10. Kumar, R. et al. (2019). Bile acid and bile acid transporters are involved in the pathogenesis of acute hepatopancreatic necrosis disease in white shrimp Litopenaeus vannamei. Cell Microbiol. doi: 10.1111/cmi.13127 (#STA-631).
  11. Palaniyandi, S.A. et al. (2019). Probiotic Characterization of Cholesterol-Lowering Lactobacillus fermentum MJM60397. Probiotics Antimicrob Proteins. doi: 10.1007/s12602-019-09585-y (#STA-631).
  12. Nikolaou, N. et al. (2019). AKR1D1 is a novel regulator of metabolic phenotype in human hepatocytes and is dysregulated in non-alcoholic fatty liver disease. Metabolism. doi: 10.1016/j.metabol.2019.153947 (#STA-631).
  13. Argemi, J. et al. (2019). Defective HNF4alpha-dependent gene expression as a driver of hepatocellular failure in alcoholic hepatitis. Nat Commun. 10(1):3126. doi: 10.1038/s41467-019-11004-3 doi: 10.1038/s41467-019-11004-3 (#STA-631).
  14. Nakada, E.M. et al. (2019). Conjugated bile acids attenuate allergen-induced airway inflammation and hyperresposiveness by inhibiting UPR transducers. JCI Insight. 4(9). pii: 98101. doi: 10.1172/jci.insight.98101 (#STA-631).
  15. Christiansen, C.B. et al. (2019). Bile acids drive colonic secretion of glucagon-like-peptide 1 and peptide-YY in rodents. Am J Physiol Gastrointest Liver Physiol. doi: 10.1152/ajpgi.00010.2019 (#STA-631).
  16. Lin, T. et al. (2019). Manipulation of the dry bean (Phaseolus vulgaris L.) matrix by hydrothermal and high-pressure treatments: Impact on in vitro bile salt-binding ability. Food Chemistry. doi: 10.1016/j.foodchem.2019.125699 (#MET-5005).
  17. Meixiong, J. et al. (2019). MRGPRX4 is a G protein-coupled receptor activated by bile acids that may contribute to cholestatic pruritus. Proc Natl Acad Sci U S A. pii: 201903316. doi: 10.1073/pnas.1903316116 (#MET-5005).
  18. Chevre, R. et al. (2018). Therapeutic modulation of the bile acid pool by Cyp8b1 knockdown protects against nonalcoholic fatty liver disease in mice. FASEB J. 32(7):3792-3802. doi: 10.1096/fj.201701084RR (#STA-631).
  19. Pereira, S.S. et al. (2018). Biliopancreatic diversion with duodenal switch (BPD-DS) and single-anastomosis duodeno-ileal bypass with sleeve gastrectomy (SADI-S) result in distinct post-prandial hormone profiles. Int J Obes (Lond). doi: 10.1038/s41366-018-0282-z (#STA-631).
  20. Ying, F. et al. (2018). EP4 emerges as a novel regulator of bile acid synthesis and its activation protects against hypercholesterolemia. Biochim Biophys Acta Mol Cell Biol Lipids. 1863(9):1029-1040. doi: 10.1016/j.bbalip.2018.06.003 (#STA-631).
  21. Buckner, S.L. et al. (2018). Di-N-octylphthalate acts as a proliferative agent in murine cell hepatocytes by regulating the levels of TGF-β and pro-apoptotic proteins. Food Chem Toxicol. 111:166-175. doi: 10.1016/j.fct.2017.11.005 (#STA-631).
  22. Kizawa, H. et al. (2017). Scaffold-free 3D bio-printed human liver tissue stably maintains metabolic functions useful for drug discovery. Biochemistry and Biophysics Reports. 10:186-191. doi: 10.1016/j.bbrep.2017.04.004 (#STA-631).
  23. Ngoh, Y-Y. The potential roles of Pinto bean (Phaseolus vulgaris cv. Pinto) bioactive peptides in regulating physiological functions: Protease activating, lipase inhibiting and bile acid binding activities. Journal of Functional Foods. 33: 67-75. doi: 10.1016/j.jff.2017.03.029 (#STA-631).
  24. Siow, H.L. et al. (2016). Structure-activity studies of protease activating, lipase inhibiting, bile acid binding and cholesterol-lowering effects of pre-screened cumin seed bioactive peptides. J. Funct. Foods 27:600-611 (#STA-631).
  25. Huang, H. et al. (2016). Red cabbage microgreens lower circulating LDL, liver cholesterol and inflammatory cytokines in mice fed a high fat diet. J. Agricult. Food Chem. 64:9161-9171 (#STA-631).
  26. Hu, X. et al. (2016). MitoNEET deficiency alleviates experimental alcoholic steatohepatitis in mice by stimulating endocrine adiponectin-FGF15 axis. J Biol Chem.  doi:10.1074/jbc.M116.737015 (#STA-631).
  27. Hirsch, N. et al. (2016). Prolonged feeding with green tea polyphenols exacerbates cholesterol-induced fatty liver disease in mice. Mol Nutr Food Res. doi:10.1002/mnfr.201600221 (#STA-631).