Total Bile Acid Assays

Total Bile Acid Assay Kit
  • Measures total bile acid content as low as 1 µM (colorimetric) or 0.4 µM (fluorometric)
  • Suitable for plasma, serum, and cell or tissue lysates
  • Available with colorimetric or fluorometric detection
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Total Bile Acid Assay Kit (Colorimetric)
Catalog Number
STA-631
Size
100 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$380.00
Total Bile Acid Assay Kit (Fluorometric)
Catalog Number
MET-5005
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$375.00
Product Details

While bile acid synthesis is critical for the removal of cholesterol from the body, bile acids are also required for proper uptake of nutrients in the small intestine. Our Total Bile Acid Assay Kit provides a convenient 96-well plate-based method to measure the total bile acid content in a variety of sample types.

These assays are based on an enzyme driven reaction in which bile acids are incubated in the presence of 3-alpha hydroxysteroiddehydrogenase. The reaction used with the colorimetric kit requires the presence of NADH, and thio-NAD+. The thio-NAD+ is reduced to thio-NADH which is detected by colorimetric absorbance. The fluorometric kit requires incubation with NAD+, which is converted to NADH. Diaphorase then uses NADH to reduce resazurin to resorufin, which is detected fluormetrically at 560nm excitation and 590nm emission.

Recent Product Citations
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  2. Dächert, C. et al. (2019). Gene Expression Profiling of Different Huh7 Variants Reveals Novel Hepatitis C Virus Host Factors. Viruses. 12(1). pii: E36. doi: 10.3390/v12010036 (#STA-631).
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  4. Torres, S.E. et al. (2019). Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether. Elife. doi: 10.7554/eLife.46595 (#STA-631).
  5. Kumar, R. et al. (2019). Bile acid and bile acid transporters are involved in the pathogenesis of acute hepatopancreatic necrosis disease in white shrimp Litopenaeus vannamei. Cell Microbiol. doi: 10.1111/cmi.13127 (#STA-631).
  6. Palaniyandi, S.A. et al. (2019). Probiotic Characterization of Cholesterol-Lowering Lactobacillus fermentum MJM60397. Probiotics Antimicrob Proteins. doi: 10.1007/s12602-019-09585-y (#STA-631).
  7. Nikolaou, N. et al. (2019). AKR1D1 is a novel regulator of metabolic phenotype in human hepatocytes and is dysregulated in non-alcoholic fatty liver disease. Metabolism. doi: 10.1016/j.metabol.2019.153947 (#STA-631).
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  9. Nakada, E.M. et al. (2019). Conjugated bile acids attenuate allergen-induced airway inflammation and hyperresposiveness by inhibiting UPR transducers. JCI Insight. 4(9). pii: 98101. doi: 10.1172/jci.insight.98101 (#STA-631).
  10. Christiansen, C.B. et al. (2019). Bile acids drive colonic secretion of glucagon-like-peptide 1 and peptide-YY in rodents. Am J Physiol Gastrointest Liver Physiol. doi: 10.1152/ajpgi.00010.2019 (#STA-631).
  11. Lin, T. et al. (2019). Manipulation of the dry bean (Phaseolus vulgaris L.) matrix by hydrothermal and high-pressure treatments: Impact on in vitro bile salt-binding ability. Food Chemistry. doi: 10.1016/j.foodchem.2019.125699 (#MET-5005).
  12. Meixiong, J. et al. (2019). MRGPRX4 is a G protein-coupled receptor activated by bile acids that may contribute to cholestatic pruritus. Proc Natl Acad Sci U S A. pii: 201903316. doi: 10.1073/pnas.1903316116 (#MET-5005).
  13. Chevre, R. et al. (2018). Therapeutic modulation of the bile acid pool by Cyp8b1 knockdown protects against nonalcoholic fatty liver disease in mice. FASEB J. 32(7):3792-3802. doi: 10.1096/fj.201701084RR (#STA-631).
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  19. Siow, H.L. et al. (2016). Structure-activity studies of protease activating, lipase inhibiting, bile acid binding and cholesterol-lowering effects of pre-screened cumin seed bioactive peptides. J. Funct. Foods 27:600-611 (#STA-631).
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