BrdU Cell Proliferation ELISA Kit

BrdU Cell Proliferation ELISA Kit
  • Detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody
  • BrdU standard included


Video: Color Development in an ELISA

Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

CytoSelect™ BrdU Cell Proliferation ELISA Kit
Catalog Number
96 assays
Manual/Data Sheet Download
SDS Download
Product Details

The CytoSelect™ BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.


Assay Principle for the CytoSelect™ BrdU Cell Proliferation ELISA Kit.

Serum Stimulation of Proliferation in HEK 293 Cells. Cells were plated overnight at 37ºC. Cells were then incubated in the presence or absence of 10% FBS for 24 hours, followed by treatment with 10 µM BrdU for 1 or 3 hours. Cells were tested for BrdU incorporation according to the assay protocol.

Recent Product Citations
  1. Houri, A. et al. (2023). Suprabasin enhances the invasion, migration, and angiogenic ability of oral squamous cell carcinoma cells under hypoxic conditions. Oncol Rep. 49(5):83. doi: 10.3892/or.2023.8520.
  2. Lee, H. et al. (2022). Biological Effects of Yeast-fermented Plant Root Extract Mixture on Skin Cells.  Asian J Beauty Cosmetol. 20(3): 349-359. doi: 10.20402/ajbc.2022.0045.
  3. Li, L. et al. (2022). Trehalose Protects Keratinocytes against Ultraviolet B Radiation by Activating Autophagy via Regulating TIMP3 and ATG9A. Oxid Med Cell Longev. doi: 10.1155/2022/9366494.
  4. Fujino, T. (2022). Transient Receptor Potential Melastatin 8, a sensor of cold temperatures mediates expression of cyclin-dependent kinase inhibitor, p21/Cip1, a regulator of epidermal cell proliferation. J Toxicol Sci. 47(3):117-123. doi: 10.2131/jts.47.117.
  5. Fan, L. et al. (2021). MicroRNA miR-23b-3p promotes osteosarcoma by targeting ventricular zone expressed PH domain-containing 1 (VEPH1)/phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway. Bioengineered. 12(2):12568-12582. doi: 10.1080/21655979.2021.2010383.
  6. Kwon, S.Y. et al. (2021). β2‑adrenergic receptor expression and the effects of norepinephrine and propranolol on various head and neck cancer subtypes. Oncol Lett. doi: 10.3892/ol.2021.13065.
  7. Soldan, S.S. et al. (2021). EBNA1 inhibitors have potent and selective antitumor activity in xenograft models of Epstein-Barr virus-associated gastric cancer. Gastric Cancer. doi: 10.1007/s10120-021-01193-6.
  8. Wu, Q. et al. (2021). Tumor suppressor role of sFRP‑4 in hepatocellular carcinoma via the Wnt/β‑catenin signaling pathway. Mol Med Rep. 23(5):336. doi: 10.3892/mmr.2021.11975.
  9. Blasetti Fantauzzi, C. et al. (2020). Galectin-3 gene deletion results in defective adipose tissue maturation and impaired insulin sensitivity and glucose homeostasis. Sci Rep. 10(1):20070. doi: 10.1038/s41598-020-76952-z.
  10. Nguyen, U.T. et al. (2020). Natural Compound Mixture, Containing Emodin, Genipin, Chlorogenic Acid, Cimigenoside, and Ginsenoside Rb1, Ameliorates Psoriasis-Like Skin Lesions by Suppressing Inflammation and Proliferation in Keratinocytes. Evid Based Complement Alternat Med. doi: 10.1155/2020/9416962.
  11. Terazawa, S. et al. (2020). Mycosporine-like amino acids stimulate hyaluronan secretion by up-regulating hyaluronan synthase 2 via activation of the p38/MSK1/CREB/c‑Fos/AP-1 axis. J Biol Chem. pii: jbc.RA119.011139. doi: 10.1074/jbc.RA119.011139.
  12. Abo-El Fetoh, M.E. et al. (2020). Cyclosporin A activates human hepatocellular carcinoma (HepG2 cells) proliferation: implication of EGFR-mediated ERK1/2 signaling pathway. Naunyn Schmiedebergs Arch Pharmacol. doi: 10.1007/s00210-019-01798-w.
  13. Cebrián, R. et al. (2019). Preclinical studies of toxicity and safety of the AS-48 bacteriocin. Journal of Advanced Research. doi: 10.1016/j.jare.2019.06.003.
  14. Dhar, S.K. et al. (2019). UVB-induced inactivation of manganese-containing superoxide dismutase promotes mitophagy via ROS-mediated mTORC2 pathway activation. J Biol Chem. pii: jbc.RA118.006595. doi: 10.1074/jbc.RA118.006595.
  15. Messick, T.E. et al. (2019). Structure-based design of small-molecule inhibitors of EBNA1 DNA binding blocks Epstein-Barr virus latent infection and tumor growth. Sci Transl Med. 11(482). pii: eaau5612. doi: 10.1126/scitranslmed.aau5612.
  16. Nguyen, L.T.H. et al. (2019). Astragali Radix and its compound formononetin ameliorate diesel particulate matter-induced skin barrier disruption by regulation of keratinocyte proliferation and apoptosis. J Ethnopharmacol. 228:132-141. doi: 10.1016/j.jep.2018.09.025.
  17. Ha, Y.J. et al. (2019). PIM-1 kinase is a novel regulator of proinflammatory cytokine-mediated responses in rheumatoid arthritis fibroblast-like synoviocytes. Rheumatology (Oxford). 58(1):154-164. doi: 10.1093/rheumatology/key261.
  18. Chen, B. et al. (2018). Co-expression of Akt1 and Wnt11 promotes the proliferation and cardiac differentiation of mesenchymal stem cells and attenuates hypoxia/reoxygenation-induced cardiomyocyte apoptosis. Biomed Pharmacother. 108:508-514. doi: 10.1016/j.biopha.2018.09.047.
  19. Yoon, J.E. et al. (2018). Senescent fibroblasts drive ageing pigmentation: ​A potential therapeutic target for senile lentigo. Theranostics. 8(17):4620-4632. doi: 10.7150/thno.26975.
  20. Bautista, W. W. et al. (2018). Hepatitis B Virus Infection of Normal Hepatic Stem/Progenitor Cells. Journal of Clinical and Experimental Hepatology. doi: 10.1016/j.jceh.2018.02.002.
  21. Beretti, F. et al. (2018). Amniotic fluid stem cell exosomes: Therapeutic perspective. Biofactors. 44(2):158-167. doi: 10.1002/biof.1407.
  22. Maglione, M. et al. (2017). In vivo evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration. J. Oral Sci. 59(2):225-232.
  23. Kim, B. S. et al. (2016). D-dopachrome tautomerase in adipose tissue inflammation and wound repair. J Cell Mol Med. doi:10.1111/jcmm.12936.
  24. Kreiseder, B. et al. (2015). Alpha-catulin contributes to drug-resistance of melanoma by activating NF-κB and AP-1. PLoS One. 10:e0119402-e0119402.
  25. Hatzis, C. et al. (2014) Enhancing Reproducibility in Cancer Drug Screening: How Do We Move Forward? Cancer Res. 74:4016-4023.