Cdc42 Activation Assays

  • Safe non-radioactive assay format
  • Colored agarose beads allow visual check
  • Fast results: 1 hour plus electrophoresis/blotting time
  • Compatible with human, mouse, and rat samples

 

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Cdc42 Activation Assay
Catalog Number
STA-402
Size
20 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$610.00
RhoA/Rac1/Cdc42 Activation Assay Combo Kit
Catalog Number
STA-405
Size
3 x 10 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$895.00
Rac1/Cdc42 Activation Assay Combo Kit
Catalog Number
STA-404
Size
2 x 20 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$895.00
Cdc42 Activation Assay Kit, Trial Size
Catalog Number
STA-402-T
Size
5 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$305.00
Product Details

Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit.

If you are also studying Rac1 or RhoA, you may consider one of our economical combination kits.

Small GTPase Activation Assay Principle

Immunoblotting with the Rac Activation Assay. Lane 1: GTPase Immunoblot Positive Control. Lane 2: 293 cell lysate loaded with GDP and incubated with PAK1 PBD Agarose beads. Lane 3: 293 cell lysate loaded with GTPγS and incubated with PAK1 PBD Agarose beads.

Recent Product Citations
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  3. Sherchan, P. et al. (2015). Recombinant Slit2 attenuates neuroinflammation after surgical brain injury by inhibiting peripheral immune cell infiltration via Robo1-srGAP1 pathway in a rat model. Neurobiol Dis. 85:164-173 (#STA-402).
  4. Sahu, M. et al. (2014). Lens specific RLIP76 transgenic mice show a phenotype similar to microphthalmia. Exp Eye Res. 118:125-134 (#STA-402).
  5. El-Sayed, F.G. et al. (2014).  P2Y2 Nucleotide Receptor Activation Enhances the Aggregation and Self-Organization of Dispersed Salivary Epithelial Cells.  Am J Physiol Cell Physiol. 307: C83-C96 (#STA-402).
  6. He, S. et al. (2013). SRGAP1 Is a Candidate Gene for Papillary Thyroid Carcinoma Susceptibility. J. Clin. Endocrinol. Metab. 98:E973-E980 (#STA-402).
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  8. Zhang, S. et al. (2008). The Tumor Suppressor LKB1 Regulates Lung Cancer Cell Polarity by  Mediating Cdc42 Recruitment and Activity. Cancer Res. 68:740-748 (#STA-402).
  9. Tanaka, U. et al. (2015). Sprouty2 inhibition promotes proliferation and migration of periodontal ligament cells. Oral Dis. doi:10.1111/odi.12369 (#STA-404).
  10. Holmes,K.M.et al.(2012).Insulin-Like Growth Factor-Binding Protein 2-Driven Glioma Progression is Prevented by Blocking a Clinically Significant Integrin, Integrin-Linked Kinase, and NF-B Network.Proc Natl Acad Sci 109:2168-2173(#STA-404).
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  12. Chen, H. et al. (2010). Integrity of SOS1/EPS8/ABI1 Tri-Complex Determines Ovarian Cancer Metastasis. Cancer Res. 70:9979-9990 (#STA-404).
  13. Pandey, D. et al. (2009). Unraveling a Novel Rac1-Mediated Signaling Pathway that Regulates Cofilin Dephosphorylation and Secretion in Thrombin Stimulated Platelets. Blood 114:415-424 (#STA-404).
  14. Lorger, M. et al. (2006). Regulation of Epithelial Wound Closure and Intercellular Adhesion by Interaction of AF6 with Actin Cytoskeleton. J. of Cell Science 119:3385-3398 (#STA-404).
  15. Prudent, J. et al. (2016). Mitochondrial Ca2+ uptake controls actin cytoskeleton dynamics during cell migration. Sci. Rep. 6:36570 (#STA-405).
  16. Le, L. T. et al. (2016). Loss of miR-203 regulates cell shape and matrix adhesion through ROBO1/Rac/FAK in response to stiffness. J Cell Biol. 212:707-719 (#STA-405).
  17. Mori, H. et al. (2015). Smad3 deficiency leads to mandibular condyle degradation via the sphingosine 1-phosphate (S1P)/S1P 3 signaling axis. Am J Pathol.  doi:10.1016/j.ajpath.2015.06.015 (#STA-405).
  18. Nasrallah, A. et al. (2014). Effect of StarD13 on colorectal cancer proliferation, motility and invasion. Oncol Rep. 31:505-515 (#STA-405).
  19. Luis-Ravelo, D. et al. (2014). RHOB influences lung adenocarcinoma metastasis and resistance in a host-sensitive manner. Mol Oncol. 8:196-206 (#STA-405).
  20. Khalil, B. D. et al. (2014). The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility. Exp Cell Res. 321:109-122 (#STA-405).
  21. Hanna, S. et al. (2014). StarD13 is a tumor suppressor in breast cancer that regulates cell motility and invasion. Int J Oncol. 44:1499-1511 (#STA-405).
  22. Wang, J. et al. (2013). DEK Depletion Negatively Regulates Rho/ROCK/MLC Pathway in Non–Small Cell Lung Cancer. Journal of Histochemistry & Cytochemistry. 10.1369/0022155413488120 (#STA-405).
  23. Quint, P. et al. (2013). Sphingosine 1-Phosphate (S1P) Receptors 1 and 2 Coordinately Induce Mesenchymal Cell Migration through S1P Activation of Complementary Kinase Pathways. J. Biol. Chem. 288:5398-5406 (#STA-405).
  24. Yu, X. et al. (2012). RhoGDI SUMOylation at Lys-138 Increases Its Binding Activity to Rho GTPase and Its Inhibiting Cancer Cell Motility. J.Biol. Chem. 287:13752-13760 (#STA-405).
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  27. Ma, T. el al. (2010). Viral G Protein-Coupled Receptor Up-Regulates Angiopoietin-like 4 Promoting Angiogenesis and Vascular Permeability in Kaposi's Aarcoma. PNAS. 104:14363-14368. (#STA-405).
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