Catalog Number | STA-402 |
Size | 20 assays |
Detection | Immunoblot |
Manual/Data Sheet | Download |
MSDS | Download |
Price | Please select your country for pricing. |
Catalog Number | STA-402-T |
Size | 5 assays |
Detection | Immunoblot |
Manual/Data Sheet | Download |
MSDS | Download |
Price | Please select your country for pricing. |
Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit.
If you are also studying Rac1 or RhoA, you may consider one of our economical combination kits.
Figure 1: Small GTPase Activation Assay Principle
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Figure 2: Immunoblotting with the Rac Activation Assay. Lane 1: GTPase Immunoblot Positive Control. Lane 2: 293 cell lysate loaded with GDP and incubated with PAK1 PBD Agarose beads. Lane 3: 293 cell lysate loaded with GTPγS and incubated with PAK1 PBD Agarose beads.
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