OxiSelect™ Nε-(carboxyethyl) lysine (CEL) ELISA Kit provides rapid detection and quantitation of CEL protein adducts. First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect™ CEL ELISA Kit will not cross react with CML protein adducts.
