Cell Adhesion Assays, ECM Array

Cell Adhesion Assays, ECM Array
  • Full quantitation of cell adhesion with no manual cell counting
  • Fluorometric or colorimetric detection
  • Plates precoated with a uniform substrate layer of a single ECM protein in each row: Collagen I, Collagen IV, Fibrinogen, Fibronectin, and Laminin

 

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CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array
Catalog Number
CBA-070
Size
48 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$515.00
CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array
Catalog Number
CBA-071
Size
48 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$555.00
CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array
Catalog Number
CBA-070-5
Size
5 x 48 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$2,275.00
CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array
Catalog Number
CBA-071-5
Size
5 x 48 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$2,430.00
Product Details

Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival.

Our CytoSelect™ 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with your choice of single ECM protein in each of the first 5 rows, with the last row provided as a negative control. Just follow a simple protocol:

  1. Cells are seeded onto the substrate.
  2. Adherent cells attach, while non-adherent cells are washed away.
  3. Adherent cells are quantified on a standard plate reader or fluorometer.

CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array. Serum starved cells from three different cell lines were allowed to attach to the ECM-coated 48-well plate for 1 hr at 100,000 cells/well. Adherent cells were stained according to the assay protocol. BSA = Bovine Serum Albumin. VN = Vitronectin. LN = Laminin. FN = Fibronectin. Col = Collagen.

Recent Product Citations
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  4. Lepsenyi, M. et al. (2021). CXCL2-CXCR2 axis mediates αV integrin-dependent peritoneal metastasis of colon cancer cells. Clin Exp Metastasis. doi: 10.1007/s10585-021-10103-0 (#CBA-071).
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  6. Takeshita, Y. et al. (2021). Angiopoietin-like protein 2 decreases peritoneal metastasis of ovarian cancer cells by suppressing anoikis resistance. Biochem Biophys Res Commun. 561:26-32. doi: 10.1016/j.bbrc.2021.05.008 (#CBA-070).
  7. Potter, J.A. et al. (2021). Collagen I Modifies Connexin-43 Hemichannel Activity via Integrin α2β1 Binding in TGFβ1-Evoked Renal Tubular Epithelial Cells. Int J Mol Sci. 22(7):3644. doi: 10.3390/ijms22073644 (#CBA-070).
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  11. Celentano, A. et al. (2020). Kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro. Sci Rep. 10(1):15904. doi: 10.1038/s41598-020-73058-4 (#CBA-070).
  12. Jha, S.K. et al. (2020). Enhanced antitumor efficacy of bile acid-lipid complex-anchored docetaxel nanoemulsion via oral metronomic scheduling. J Control Release. 328:368-394. doi: 10.1016/j.jconrel.2020.08.067 (#CBA-070).
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  15. Okada, J. et al. (2020). Dapagliflozin Inhibits Cell Adhesion to Collagen I and IV and Increases Ectodomain Proteolytic Cleavage of DDR1 by Increasing ADAM10 Activity. Molecules. 25(3). pii: E495. doi: 10.3390/molecules25030495 (#CBA-070).
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  17. Liu, L.Q. et al. (2019). MiR-92a antagonized the facilitation effect of extracellular matrix protein 1 in GC metastasis through targeting its 3'UTR region. Food Chem Toxicol. 133:110779. doi: 10.1016/j.fct.2019.110779 (#CBA-070).
  18. Hou, S. et al. (2019). Integrin α5 promotes migration and cisplatin resistance in esophageal squamous cell carcinoma cells. Am J Cancer Res. 9(12):2774-2788 (#CBA-070).
  19. Uno, Y. et al. (2019). Expression, Function, and Prognostic Value of MAGE-D4 Protein in Esophageal Squamous Cell Carcinoma. Anticancer Res. 39(11):6015-6023. doi: 10.21873/anticanres.13807 (#CBA-070).
  20. Umeda, S. et al. (2019). Fraser extracellular matrix complex subunit 1 promotes liver metastasis of gastric cancer. Int J Cancer. doi: 10.1002/ijc.32705 (#CBA-070).
  21. Hirano, K. et al. (2019). LacdiNAcylation of N-glycans in MDA-MB-231 human breast cancer cells results in changes in morphological appearance and adhesive properties of the cells. Histochem Cell Biol. doi: 10.1007/s00418-019-01822-3 (#CBA-070).
  22. Ding, L. et al. (2019). Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ. Nat Commun. 10(1):4182. doi: 10.1038/s41467-019-12125-5 (#CBA-070).
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  26. Sawaki, K. et al. (2019). Level of Melanotransferrin in Tissue and Sera Serves as a Prognostic Marker of Gastric Cancer. Anticancer Res. 39(11):6125-6133. doi: 10.21873/anticanres.13820 (#CBA-071).
  27. Maxwell, J.T. et al. (2019). Electrical stimulation of pediatric cardiac-derived c-kit+ progenitor cells improves retention and cardiac function in right ventricular heart failure. Stem Cells. doi: 10.1002/stem.3088 (#CBA-071).
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