Cell Adhesion Assays, ECM Array

Cell Adhesion Assays, ECM Array
  • Full quantitation of cell adhesion with no manual cell counting
  • Fluorometric or colorimetric detection
  • Plates precoated with a uniform substrate layer of a single ECM protein in each row: Collagen I, Collagen IV, Fibrinogen, Fibronectin, and Laminin

 

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CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array
Catalog Number
CBA-070
Size
48 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$470.00
CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array
Catalog Number
CBA-071
Size
48 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$500.00
CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array
Catalog Number
CBA-070-5
Size
5 x 48 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$2,050.00
CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array
Catalog Number
CBA-071-5
Size
5 x 48 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$2,205.00
Product Details

Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival.

Our CytoSelect™ 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with your choice of single ECM protein in each of the first 5 rows, with the last row provided as a negative control. Just follow a simple protocol:

  1. Cells are seeded onto the substrate.
  2. Adherent cells attach, while non-adherent cells are washed away.
  3. Adherent cells are quantified on a standard plate reader or fluorometer.

CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array. Serum starved cells from three different cell lines were allowed to attach to the ECM-coated 48-well plate for 1 hr at 100,000 cells/well. Adherent cells were stained according to the assay protocol. BSA = Bovine Serum Albumin. VN = Vitronectin. LN = Laminin. FN = Fibronectin. Col = Collagen.

Recent Product Citations
  1. Okada, J. et al. (2020). Dapagliflozin Inhibits Cell Adhesion to Collagen I and IV and Increases Ectodomain Proteolytic Cleavage of DDR1 by Increasing ADAM10 Activity. Molecules. 25(3). pii: E495. doi: 10.3390/molecules25030495 (#CBA-070).
  2. Nakamura, S. et al. (2020). KCNJ15 Expression and Malignant Behavior of Esophageal Squamous Cell Carcinoma. Ann Surg Oncol. doi: 10.1245/s10434-019-08189-8 (#CBA-071).
  3. Liu, L.Q. et al. (2019). MiR-92a antagonized the facilitation effect of extracellular matrix protein 1 in GC metastasis through targeting its 3'UTR region. Food Chem Toxicol. 133:110779. doi: 10.1016/j.fct.2019.110779 (#CBA-070).
  4. Hou, S. et al. (2019). Integrin α5 promotes migration and cisplatin resistance in esophageal squamous cell carcinoma cells. Am J Cancer Res. 9(12):2774-2788 (#CBA-070).
  5. Uno, Y. et al. (2019). Expression, Function, and Prognostic Value of MAGE-D4 Protein in Esophageal Squamous Cell Carcinoma. Anticancer Res. 39(11):6015-6023. doi: 10.21873/anticanres.13807 (#CBA-070).
  6. Umeda, S. et al. (2019). Fraser extracellular matrix complex subunit 1 promotes liver metastasis of gastric cancer. Int J Cancer. doi: 10.1002/ijc.32705 (#CBA-070).
  7. Hirano, K. et al. (2019). LacdiNAcylation of N-glycans in MDA-MB-231 human breast cancer cells results in changes in morphological appearance and adhesive properties of the cells. Histochem Cell Biol. doi: 10.1007/s00418-019-01822-3 (#CBA-070).
  8. Ding, L. et al. (2019). Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ. Nat Commun. 10(1):4182. doi: 10.1038/s41467-019-12125-5 (#CBA-070).
  9. Birtley, J.R. et al. (2019). Inactivating mutations and X-ray crystal structure of the tumor suppressor OPCML reveal cancer-associated functions. Nat Commun. 10(1):3134. doi: 10.1038/s41467-019-10966-8 (#CBA-070).
  10. Sohn, H.J. et al. (2019). Cellular characterization of actin gene concerned with contact-dependent mechanisms in Naegleria fowleri. Parasite Immunol. e12631. doi: 10.1111/pim.12631 (#CBA-070).
  11. Babchia, N. et al. (2019). The bidirectional crosstalk between metastatic uveal melanoma cells and hepatic stellate cells engenders an inflammatory microenvironment. Exp Eye Res. 181:213-222. doi: 10.1016/j.exer.2019.02.012 (#CBA-070).
  12. Zhao, X. et al. (2019). Intracellular reduction in ATP levels contributes to CYT997-induced suppression of metastasis of head and neck squamous carcinoma. J Cell Mol Med. 23(2):1174-1182. doi: 10.1111/jcmm.14017 (#CBA-070).
  13. Arzmi, M.H. et al. (2019). Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells. Carcinogenesis. 40(1):184-193. doi: 10.1093/carcin/bgy137 (#CBA-070).
  14. Sun, H.F. et al. (2019). Loss of TMEM126A promotes extracellular matrix remodeling, epithelial-to-mesenchymal transition, and breast cancer metastasis by regulating mitochondrial retrograde signaling. Cancer Lett. 440-441:189-201. doi: 10.1016/j.canlet.2018.10.018 (#CBA-070).
  15. Sawaki, K. et al. (2019). Level of Melanotransferrin in Tissue and Sera Serves as a Prognostic Marker of Gastric Cancer. Anticancer Res. 39(11):6125-6133. doi: 10.21873/anticanres.13820 (#CBA-071).
  16. Maxwell, J.T. et al. (2019). Electrical stimulation of pediatric cardiac-derived c-kit+ progenitor cells improves retention and cardiac function in right ventricular heart failure. Stem Cells. doi: 10.1002/stem.3088 (#CBA-071).
  17. Uno, Y. et al. (2019). Increased Expression of DNAJC12 is Associated with Aggressive Phenotype of Gastric Cancer. Ann Surg Oncol. 26(3):836-844. doi: 10.1245/s10434-018-07149-y (#CBA-071).
  18. Itoh, H. et al. (2018). Endometrial stromal cell attachment and matrix homeostasis in abdominal wall endometriomas. Hum Reprod. 33(2):280-291. doi: 10.1093/humrep/dex371 (#CBA-070).
  19. Chong, K. et al. (2018). Inflammation by Activated Macrophage-Like THP-1 Cells Increases Human Dura Mater Cell Adhesion with Alteration of Integrin α2 β1 and Matrix Metalloproteinase. Journal of Orthopaedic Research®. doi:10.1002/jor.24207 (#CBA-070).
  20. Zhou, M. et al. (2018). Caspase-3 regulates the migration, invasion and metastasis of colon cancer cells. Int J Cancer. 143(4):921-930. doi: 10.1002/ijc.31374 (#CBA-070).
  21. Hu, L. et al. (2018). G9A promotes gastric cancer metastasis by upregulating ITGB3 in a SET domain-independent manner. Cell Death Dis. 9(3):278. doi: 10.1038/s41419-018-0322-6 (#CBA-070).
  22. Manders, D.B. et al. (2018). Dysregulation of fibulin-5 and matrix metalloproteases in epithelial ovarian cancer. Oncotarget. 9(18):14251-14267. doi: 10.18632/oncotarget.24484 (#CBA-070).
  23. Baldión, P.A. et al. (2018). Odontoblast-Like Cells Differentiated from Dental Pulp Stem Cells Retain Their Phenotype after Subcultivation. Int J Cell Biol. 2018:6853189. doi: 10.1155/2018/6853189 (#CBA-070).
  24. Andriamoratsiresy, D. et al. (2018). PFN2a, a new partner of RARα in the cytoplasm. Biochem Biophys Res Commun. 495(1):846-853. doi: 10.1016/j.bbrc.2017.11.096 (#CBA-070).
  25. Moore, L.M. et al. (2017). Calcium Sensor, NCS-1, Promotes Tumor Aggressiveness and Predicts Patient Survival. Mol Cancer Res. pii: molcanres.0408.2016. doi: 10.1158/1541-7786.MCR-16-0408 (#CBA-070).
  26. Guerra, A.D. et al. (2017). Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels. Acta Biomater. doi: 10.1016/j.actbio.2017.01.021 (#CBA-070).
  27. Bradbury, P.M. et al. (2017). The focal adhesion targeting (FAT) domain of p130 Crk associated substrate (p130Cas) confers mechanosensing function. J Cell Sci. doi: 10.1242/jcs.192930 (#CBA-070).
  28. Ponda, M.P. and Breslow, J.L. (2016). Serum stimulation of CCR7 chemotaxis due to coagulation factor XIIa-dependent production of high-molecular-weight kininogen domain 5. PNAS 113:E7059-E7068 (#CBA-070).
  29. Zohra, F. T. et al. (2015). Functional behavior and gene expression of magnetic nanoparticle-loaded primary endothelial cells for targeting vascular stents. Nanomedicine (Lond). 10:1391-1406 (#CBA-071).
  30. Kim, S.W. et al. (2013). Cardiac Stem Cells with Electrical Stimulation Improve Ischaemic Heart Function through Regulation of Connective Tissue Growth Factor and miR-378. Cardiovasc Res. 10.1093/cvr/cvt192 (#CBA-071).