Cell Contraction Assays, Two-Step Attached Model

Cell Contraction Assays, Two-Step Attached Model
  • Uses a 3D collagen matrix to measure changes in the collagen gel size
  • Assess cell contractivity and screen for cell contraction mediators
  • Includes optional cell contraction inhibitor


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Cell Contraction Assay
Catalog Number
24 assays
Light Microscopy
Manual/Data Sheet Download
SDS Download
Product Details

The two-step attached model combines an initial period of attached matrix contraction leading to mechanical loading, followed by release of the matrices, resulting in mechanical unloading and further contraction as mechanical stress dissipates. Cell Biolabs’ Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators.

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  1. Bharadwaj, A. et al. (2023). Musculoskeletal defects associated with myosin heavy chain-embryonic loss of function are mediated by the YAP signaling pathway. EMBO Mol Med. 15(9):e17187. doi: 10.15252/emmm.202217187.
  2. Zhang, H. et al. (2023). Enhanced Cerebral Hemodynamics and Cognitive Function Via Knockout of Dual-Specificity Protein Phosphatase 5. J Pharm Pharmacol Res. doi: 10.26502/fjppr.070.
  3. Ji, K. et al. (2023). Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour. Clin Transl Med. 13(4):e1234. doi: 10.1002/ctm2.1234. 
  4. Li, F.Q. et al. (2023). Bone marrow mesenchymal stem cell-derived exosomal microRNAs target PI3K/Akt signaling pathway to promote the activation of fibroblasts. World J Stem Cells. 15(4):248-267. doi: 10.4252/wjsc.v15.i4.248.
  5. Yang, H.M. et al. (2023). Identification of cell-biologic mechanisms of coronary artery spasm and its ex vivo diagnosis using peripheral blood-derived iPSCs. Biomater Res. 27(1):16. doi: 10.1186/s40824-023-00345-2.
  6. Zhang, Y. et al. (2022). TIPARP is involved in the regulation of intraocular pressure. Commun Biol. 5(1):1386. doi: 10.1038/s42003-022-04346-0.
  7. Ebrahimpour, A. et al. (2022). Combination of esomeprazole and pirfenidone enhances antifibrotic efficacy in vitro and in a mouse model of TGFβ-induced lung fibrosis. Sci Rep. 12(1):20668. doi: 10.1038/s41598-022-24985-x.
  8. Diao, M. et al. (2022). RAC1 is involved in uterine myometrium contraction in the inflammation-associated preterm birth. Reproduction. 164(4):169-181. doi: 10.1530/REP-21-0186.
  9. Zada, A. et al. (2022). The long Filamin-a isoform is required for intestinal development and motility: implications for chronic intestinal pseudo-obstruction. Hum Mol Genet. doi: 10.1093/hmg/ddac199.
  10. Sheng, F. et al. (2022). Poly amino acid thermosensitive hydrogel loaded with ICG-001 for inhibiting keloid by down-regulating the Wnt/β-Catenin pathway. Mater Des. doi: 10.1016/j.matdes.2022.111050.
  11. Böker, V. et al. (2022). Analysis of genomic alterations in cancer associated human pancreatic stellate cells. Sci Rep. 12(1):13532. doi: 10.1038/s41598-022-17748-1.
  12. Do, D.V. et al. (2022). The Effects of Irisin on the Interaction between Hepatic Stellate Cell and Macrophage in Liver Fibrosis. Endocrinol Metab (Seoul). doi: 10.3803/EnM.2022.1412.
  13. Wang, S. et al. (2021). Luseogliflozin, a sodium-glucose co-transporter 2 inhibitor, reverses cerebrovascular dysfunction and cognitive impairments in 18-month-old diabetic animals. Am J Physiol Heart Circ Physiol. doi: 10.1152/ajpheart.00438.2021.
  14. Wei, Y. et al. (2021). Adrenomedullin ameliorates pulmonary fibrosis by regulating TGF-β-Smads signaling and myofibroblast differentiation. Endocrinology. doi: 10.1210/endocr/bqab090.
  15. Zou, Q. et al. (2022). Small extracellular vesicles derived from dermal fibroblasts promote fibroblast activity and skin development through carrying miR-218 and ITGBL1. J Nanobiotechnology. 20(1):296. doi: 10.1186/s12951-022-01499-2.
  16. Hao, M. et al. (2022). Activation of α7 nicotinic acetylcholine receptor retards the development of endometriosis. Reprod Biol Endocrinol. 20(1):85. doi: 10.1186/s12958-022-00955-w.
  17. Wang, S. et al. (2022). Upregulation of GLT25D1 in Hepatic Stellate Cells Promotes Liver Fibrosis via the TGF-β1/SMAD3 Pathway In Vivo and In vitro. J Clin Transl Hepatol. doi: 10.14218/JCTH.2022.00005.
  18. Lee, K.W. et al. (2022). PRRX1 is a master transcription factor of stromal fibroblasts for myofibroblastic lineage progression. Nat Commun. 13(1):2793. doi: 10.1038/s41467-022-30484-4.
  19. Huang, S. et al. (2022). Tetramethylpyrazine Retards the Progression and Fibrogenesis of Endometriosis. Reprod Sci. doi: 10.1007/s43032-021-00813-x.
  20. Morales, M.M. et al. (2022). A 2D and 3D cell culture protocol to study O-GlcNAc in sphingosine-1-phosphate mediated fibroblast contraction. STAR Protoc. doi: 10.1016/j.xpro.2021.101113.
  21. Kim, J.H. et al. (2021). Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. 12(1):6537. doi: 10.1038/s41467-021-26900-w.
  22. Lecce, L. et al. (2021). Histone deacetylase 9 promotes endothelial-mesenchymal transition and an unfavorable atherosclerotic plaque phenotype. J Clin Invest. 131(15):e131178. doi: 10.1172/JCI131178.
  23. Xiao, F. et al. (2021). Tumor-Suppressing STF cDNA 3 Overexpression Suppresses Renal Fibrosis by Alleviating Anoikis Resistance and Inhibiting the PI3K/Akt Pathway. Kidney Blood Press Res. doi: 10.1159/000517318.
  24. Yokota, M. et al. (2021). Staphylococcus aureus impairs dermal fibroblast functions with deleterious effects on wound healing. FASEB J. 35(7):e21695. doi: 10.1096/fj.201902836R.
  25. Morales, M.M. et al. (2021). O-GlcNAc modification of MYPT1 modulates lysophosphatidic acid (LPA)-induced cell contraction in fibroblasts. J Biol Chem. doi: 10.1016/j.jbc.2021.100800.
  26. Zhang, L. et al. (2021). Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy. Cell Death Dis. 12(5):470. doi: 10.1038/s41419-021-03750-4.
  27. Liu, P. et al. (2021). Fasudil Dichloroacetate Alleviates SU5416/Hypoxia-Induced Pulmonary Arterial Hypertension by Ameliorating Dysfunction of Pulmonary Arterial Smooth Muscle Cells. Drug Des Devel Ther. 15:1653-1666. doi: 10.2147/DDDT.S297500.
  28. Liu, Y. et al. (2021). 20-HETE-promoted cerebral blood flow autoregulation is associated with enhanced pericyte contractility. Prostaglandins Other Lipid Mediat. 154:106548. doi: 10.1016/j.prostaglandins.2021.106548.
  29. Kim, G.R. et al. (2021). MicroRNA-212-5p and its target PAFAH1B2 suppress vascular proliferation and contraction via the downregulation of RhoA. PLoS One. 16(3):e0249146. doi: 10.1371/journal.pone.0249146.
  30. Uemura, M. et al. (2021). Monophasic Pulsed Current Stimulation of Duty Cycle 10% Promotes Differentiation of Human Dermal Fibroblasts into Myofibroblasts. Phys Ther Res. doi: 10.1298/ptr.E10064.