Cell Contraction Assays, Two-Step Attached Model

Cell Contraction Assays, Two-Step Attached Model
  • Uses a 3D collagen matrix to measure changes in the collagen gel size
  • Assess cell contractivity and screen for cell contraction mediators
  • Includes optional cell contraction inhibitor
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Cell Contraction Assay
Catalog Number
24 assays
Light Microscopy
Manual/Data Sheet Download
SDS Download
Product Details

The two-step attached model combines an initial period of attached matrix contraction leading to mechanical loading, followed by release of the matrices, resulting in mechanical unloading and further contraction as mechanical stress dissipates. Cell Biolabs’ Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators.

Recent Product Citations
  1. Pedowitz, N.J. et al. (2020). MYPT1 O-GlcNAc modification regulates sphingosine-1-phosphate mediated contraction. Nat Chem Biol. doi: 10.1038/s41589-020-0640-8.
  2. Yan, D. et al. (2020). Mesothelial Cells Participate in Endometriosis Fibrogenesis Through Platelet-Induced Mesothelial-Mesenchymal Transition. J Clin Endocrinol Metab. doi: 10.1210/clinem/dgaa550.
  3. Ilg, M.M. et al. (2020). Phosphodiesterase Type 5 Inhibitors and Selective Estrogen Receptor Modulators Can Prevent But Not Reverse Myofibroblast Transformation in Peyronie's. J Sex Med. S1743-6095(20)30727-X. doi: 10.1016/j.jsxm.2020.06.022.
  4. Wójcik-Pszczoła, K. et al. (2020). A Novel, Pan-PDE Inhibitor Exerts Anti-Fibrotic Effects in Human Lung Fibroblasts via Inhibition of TGF-β Signaling and Activation of cAMP/PKA Signaling. Int J Mol Sci. 21(11):4008. doi: 10.3390/ijms21114008.
  5. Lin, H. et al. (2020). Anti-fibrotic effect of iguratimod on pulmonary fibrosis by inhibiting the fibroblast-to-myofibroblast transition. Adv Med Sci. 65(2):338-347. doi: 10.1016/j.advms.2020.05.006.
  6. Chow, A. et al. (2020). Dasatinib inhibits peripapillary scleral myofibroblast differentiation. Exp Eye Res. doi: 10.1016/j.exer.2020.107999.
  7. Wang, S. et al. (2020). Sex differences in the structure and function of rat middle cerebral arteries. Am J Physiol Heart Circ Physiol. doi: 10.1152/ajpheart.00722.2019.
  8. Lee, E. et al. (2020). New screening system using Twist1 promoter activity identifies dihydrorotenone as a potent drug targeting cancer-associated fibroblasts. Sci Rep. 10(1):7058. doi: 10.1038/s41598-020-63996-4.
  9. Guo, Y. et al. (2020). Accelerated cerebral vascular injury in diabetes is associated with vascular smooth muscle cell dysfunction. Geroscience. doi: 10.1007/s11357-020-00179-z.
  10. Chen, G. et al. (2020). Monocyte Chemotactic Protein-1 Regulates Proliferation and Contractility of Human Bladder Smooth Muscle Cells Under Hydrostatic Pressure. J Interferon Cytokine Res. doi: 10.1089/jir.2019.0186.
  11. Zhang, H. et al. (2020). Influence of dual-specificity protein phosphatase 5 on mechanical properties of rat cerebral and renal arterioles. Physiol Rep. 8(2):e14345. doi: 10.14814/phy2.14345.
  12. Jeong, H. et al. (2020). Sustained Nitric Oxide-Providing Small Molecule and Precise Release Behavior Study for Glaucoma Treatment. Mol Pharm. doi: 10.1021/acs.molpharmaceut.9b01137.
  13. Navarro, J. et al. (2019). In Vivo Evaluation of 3D Printed, Keratin-Based Hydrogels in a Porcine Thermal Burn Model. Tissue Eng Part A. doi: 10.1089/ten.TEA.2019.0181.
  14. Malhotra, R. et al. (2019). HDAC9 is implicated in atherosclerotic aortic calcification and affects vascular smooth muscle cell phenotype. Nat Genet. doi: 10.1038/s41588-019-0514-8.
  15. Shao, T. et al. (2019). Research on Function and Mechanisms of a Novel Small Molecule WG449E for Hypertrophic Scar. J Eur Acad Dermatol Venereol. doi: 10.1111/jdv.16028.
  16. Heffer, A.M. et al. (2019). The polyether ionophore salinomycin targets multiple cellular pathways to block proliferative vitreoretinopathy pathology. PLoS One. 14(9):e0222596. doi: 10.1371/journal.pone.0222596.
  17. Esteban-Vives, R. et al. (2019). Isolation and Characterization of a Human Fetal Mesenchymal Stem Cell Population: Exploring the Potential for Cell Banking in Wound Healing Therapies. Cell Transplant. doi: 10.1177/0963689718817524.
  18. Wen, J. et al. (2019). Inhibition of LPA1 Signaling Impedes Conversion of Human Tenon's Fibroblasts into Myofibroblasts Via Suppressing TGF-β/Smad2/3 Signaling. J Ocul Pharmacol Ther. 35(6):331-340. doi: 10.1089/jop.2018.0120.
  19. Canugovi, C. et al. (2019). Increased mitochondrial NADPH oxidase 4 (NOX4) expression in aging is a causative factor in aortic stiffening. Redox Biology. doi:10.1016/j.redox.2019.101288.
  20. Lai, J. et al. (2019). β-Adrenoceptor signaling regulates proliferation and contraction of human bladder smooth muscle cells under pathological hydrostatic pressure. J Cell Biochem. doi: 10.1002/jcb.29056.
  21. Chen, L. et al. (2019). Increased Complement 3 With Suppression of miR-145 Induces the Synthetic Phenotype in Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats. J Am Heart Assoc. 8(10):e012327. doi: 10.1161/JAHA.119.012327.
  22. Yan, D. et al. (2019). Neuropeptides Substance P and Calcitonin Gene Related Peptide Accelerate the Development and Fibrogenesis of Endometriosis. Sci Rep. 9(1):2698. doi: 10.1038/s41598-019-39170-w.
  23. Cavin, S. et al. (2019). Vascular-targeted low dose photodynamic therapy stabilizes tumor vessels by modulating pericyte contractility. Lasers Surg Med. doi: 10.1002/lsm.23069.
  24. Tsou, P.S. et al. (2019). Inhibition of EZH2 prevents fibrosis and restores normal angiogenesis in scleroderma. Proc Natl Acad Sci U S A. 116(9):3695-3702. doi: 10.1073/pnas.1813006116.
  25. Fratter, A. et al. (2019). Lipomatrix: A Novel Ascorbyl Palmitate-Based Lipid Matrix to Enhancing Enteric Absorption of Serenoa Repens Oil. Int J Mol Sci. 20(3). pii: E669. doi: 10.3390/ijms20030669.
  26. Paul, M. et al. (2019). Protease‐activated receptor 2 activates CRAC‐mediated Ca 2+ influx to cause prostate smooth muscle contraction. FASEB BioAdvances. 1(4):255-264. doi:10.1096/fba.2018-00024.
  27. Wilkinson, A.W. et al. (2019). SETD3 is an actin histidine methyltransferase that prevents primary dystocia. Nature. 565(7739):372-376. doi: 10.1038/s41586-018-0821-8.
  28. Anamthathmakula, P. et al. (2019). Estrogen receptor alpha isoform ERdelta7 in myometrium modulates uterine quiescence during pregnancy. EBioMedicine. 39:520-530. doi: 10.1016/j.ebiom.2018.11.038.
  29. Ilg, M.M. et al. (2019). Antifibrotic Synergy Between Phosphodiesterase Type 5 Inhibitors and Selective Oestrogen Receptor Modulators in Peyronie's Disease Models. Eur Urol. 75(2):329-340. doi: 10.1016/j.eururo.2018.10.014.
  30. Enzo, M.V. et al. (2019). Growth rate and myofibroblast differentiation of desmoid fibroblast-like cells are modulated by TGF-β signaling. Histochem Cell Biol. 151(2):145-160. doi: 10.1007/s00418-018-1718-1.