CPD and 6-4PP ELISA Combo Kit

CPD and 6-4PP ELISA Combo Kit
  • Combo kit measures both CPD and 6-4PP products on a single plate, but in separate wells
  • Detects UV-damage structures from isolated DNA in standard ELISA plate format

 

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OxiSelect™ UV-Induced DNA Damage ELISA Combo Kit (CPD / 6-4PP Quantitation)
Catalog Number
STA-322-C
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$690.00
Product Details

Our OxiSelect™ UV-Induced DNA Damage ELISA Combo Kit measures the formation of both CPD and 6-4PP structures in DNA isolated from cells or tissues. Standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA binding plate.  The sample or standard are then probed with an anti-6-4PP or CPD antibody, followed by an HRP conjugated secondary antibody. CPD and 6-4PP structures are assayed on the same plate but in separate wells.

DNA Damage Induced by UV Light. Calf thymus DNA at 0.2 mg/mL was exposed to UV light inside a cell culture hood for the time indicated. The CPD levels in 40 ng denatured DNA samples were determined as described in the Assay Protocol for CPD.

DNA Damage Induced by UV Light. Calf thymus DNA at 0.2 mg/mL was exposed to UV light inside a cell culture hood for the time indicated. The 6-4PP levels in 40 ng denatured DNA samples were determined as described in the Assay Protocol for 6-4PP.

Recent Product Citations
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  2. Helalat, S.H. et al. (2020). Investigating the efficacy of UVSE protein at repairing CPD and 6–4 pp DNA damages in human cells. J Photochem Photobiol B. doi: 10.1016/j.jphotobiol.2020.111843.
  3. Wang, J. et al. (2020). Acetylation of XPF by TIP60 facilitates XPF-ERCC1 complex assembly and activation. Nat Commun. 11(1):786. doi: 10.1038/s41467-020-14564-x.
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  5. Ben-Zvi, O. et al. (2019). Response of fluorescence morphs of the mesophotic coral Euphyllia paradivisa to ultra-violet radiation. Sci Rep. 9(1):5245. doi: 10.1038/s41598-019-41710-3.
  6. Tong, L. et al. (2018). The Mechanisms of Carnosol in Chemoprevention of Ultraviolet B-Light-Induced Non-Melanoma Skin Cancer Formation. Sci Rep. 8(1):3574. doi: 10.1038/s41598-018-22029-x.
  7. Fang, C. et al. (2016). Methyl-CpG binding domain protein acts to regulate the repair of cyclobutane pyrimidine dimers on rice DNA. Sci Rep. doi:10.1038/srep34569.
  8. Gao, L. et al. (2015). The tomato DDI2, a PCNA ortholog, associating with DDB1-CUL4 complex is required for UV-damaged DNA repair and plant tolerance to UV stress. Plant Science. 235:101-110. 
  9. Fujimori, N. et al. (2014). Plant DNA-damage repair/toleration 100 protein repairs UV-B-induced DNA damage. DNA Repair (Amst). 21:171-176.