Global DNA Methylation ELISA

Global DNA Methylation ELISA
  • For detection and quantitation of 5'-methyl-2'-deoxycytidine (5MedCyd)
  • Suitable for use with DNA samples such as cell or tissue genomic DNA
  • Detection sensitivity of 150 nM 5MedCyd

 

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Video: Color Development in an ELISA

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Global DNA Methylation ELISA Kit (5'-methyl-2'-deoxycytidine Quantitation)
Catalog Number
STA-380
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$505.00
Global DNA Methylation ELISA Kit (5'-methyl-2'-deoxycytidine Quantitation)
Catalog Number
STA-380-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$2,175.00
Product Details

The Global DNA Methylation ELISA Kit is a competitive ELISA for the quantitative measurement of 5-methylcytosine (5MedCyd). The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.

5MedCyd ELISA Standard Curve.

5MedCyd Levels in Various Dilutions of Human Urine.

Recent Product Citations
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  8. Manzoni, E.F.M. et al. (2016). 5-azacytidine affects TET2 and histone transcription and reshapes morphology of human skin fibroblasts. Sci. Rep. 6:37017.
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  10. Shah, S. M. et al. (2016). Testicular cell conditioned medium supports embryonic stem cell differentiation towards germ lineage and to spermatocyte-and oocyte-like cells. Theriogenology. doi:10.1016/j.theriogenology.2016.02.025.
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