HBV Core Antigen ELISA

HBV Core Antigen ELISA
  • Measures the core antigen of Hepatitis B virus
  • Sensitive to as low as 1 ng/mL
  • Recombinant HBVcAg standard included

 

Video: Color Development in an ELISA

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QuickTiter™ HBV Core Antigen ELISA Kit
Catalog Number
VPK-150-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$3,450.00
QuickTiter™ HBV Core Antigen ELISA Kit
Catalog Number
VPK-150
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$795.00
Product Details

An anti-HBVcAg monoclonal coating antibody is adsorbed onto a microtiter plate. HBV core antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-HBVcAg antibody is added and binds to the antigen captured by the first antibody.

Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-HBVcAg. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells.

A colored product is formed in proportion to the amount of HBV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HBV core antigen and sample concentration is then determined.

Recent Product Citations
  1. Miyakawa, K. et al. (2022). Galectin-9 restricts hepatitis B virus replication via p62/SQSTM1-mediated selective autophagy of viral core proteins. Nat Commun. 13(1):531. doi: 10.1038/s41467-022-28171-5.
  2. Domfeh, S.A. et al. (2021). Cryptolepine and Nibima inhibit hepatitis B virus replication. Sci Afr. doi: 10.1016/j.sciaf.2021.e00942.
  3. Kong, L. et al. (2021). The synthetic opioid fentanyl enhances viral replication in vitro. PLoS One. 16(4):e0249581. doi: 10.1371/journal.pone.0249581.
  4. Akahori, Y. et al. (2020). Establishment of a novel hepatitis B virus culture system using immortalized human hepatocytes. Sci Rep. 10(1):21718. doi: 10.1038/s41598-020-78655-x.
  5. Peiffer, K.H. et al. (2020). Quadruple mutation GCAC1809-1812TTCT acts as a biomarker in healthy European HBV carriers. JCI Insight. doi: 10.1172/jci.insight.135833.
  6. Jiang, B. et al. (2020). The N-Terminus Makes the Difference: Impact of Genotype-Specific Disparities in the N-Terminal Part of The Hepatitis B Virus Large Surface Protein on Morphogenesis of Viral and Subviral Particles. Cells. 9(8):E1898. doi: 10.3390/cells9081898.
  7. Colant, N. et al. (2020). A rational approach to improving titer in E. coli-based cell-free protein synthesis reactions. Biotechnol Prog. doi: 10.1002/btpr.3062.
  8. Jiang, B. et al. (2019). Formation of semi-enveloped particles as a unique feature of a hepatitis B virus PreS1 deletion mutant. Aliment Pharmacol Ther. doi: 10.1111/apt.15381.
  9. Biswas, B. et al. (2017). A G-quadruplex motif in an envelope gene promoter regulates transcription and virion secretion in HBV genotype B. Nucleic Acids Res. 45(19):11268-11280. doi: 10.1093/nar/gkx823.