His-Tag Protein ELISA

His-Tag Protein ELISA
  • Quantify proteins with His-tag at either N-terminus or C-terminus
  • Detect as low as 1 ng/mL of protein or 50 pM 6xHis-tag residues
  • Suitable for cell or tissue lysates


Frequently Asked Questions about this product

Video: Color Development in an ELISA

Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

His-Tag Protein ELISA Kit
Catalog Number
96 assays
Manual/Data Sheet Download
SDS Download
Product Details

Our His-Tag Protein ELISA Kit allows you to detect and quantify His-tagged protein samples simply and reliably by comparing your unknown samples to a known recombinant standard. Sensitivity range of the kit is 4 µg/mL to 1 ng/mL, or 950 nM to 50 pM of 6xHis-tag residues.

Recent Product Citations
  1. Komagata, O. et al. (2021). Common substitution mutation F348Y of acetylcholinesterase gene contributes to organophosphate and carbamate resistance in Cimex lectularius and C. hemipterus. Insect Biochem Mol Biol. 138:103637. doi: 10.1016/j.ibmb.2021.103637.
  2. Hamdan, F. et al. (2021). Novel oncolytic adenovirus expressing enhanced cross-hybrid IgGA Fc PD-L1 inhibitor activates multiple immune effector populations leading to enhanced tumor killing in vitro, in vivo and with patient-derived tumor organoids. J Immunother Cancer. 9(8):e003000. doi: 10.1136/jitc-2021-003000. 
  3. Krajewska, M. et al. (2021). Solubilization, purification, and functional reconstitution of human ROMK potassium channel in copolymer styrene-maleic acid (SMA) nanodiscs. Biochim Biophys Acta Biomembr. doi: 10.1016/j.bbamem.2021.183555.
  4. Helalat, S.H. et al. (2020). Investigating the efficacy of UVSE protein at repairing CPD and 6–4 pp DNA damages in human cells. J Photochem Photobiol B. doi: 10.1016/j.jphotobiol.2020.111843.
  5. Huang, Y. et al. (2020). Dual-mechanism based CTLs infiltration enhancement initiated by Nano-sapper potentiates immunotherapy against immune-excluded tumors. Nat Commun. 11(1):622. doi: 10.1038/s41467-020-14425-7.
  6. Karamitros, C.S. et al. (2020). Bacterial expression systems for enzymatic activity in droplet-based microfluidics. Anal Chem. doi: 10.1021/acs.analchem.9b04969.
  7. Arnett, C. et al. (2019). Expression and secretion of active Moringa oleifera coagulant protein in Bacillus subtilis. Appl Microbiol Biotechnol. doi: 10.1007/s00253-019-10141-5.
  8. Liu, Q. et al. (2018). Nanoparticle-Mediated Trapping of Wnt Family Member 5A in Tumor Microenvironments Enhances Immunotherapy for B-Raf Proto-Oncogene Mutant Melanoma. ACS Nano. 12(2):1250-1261. doi: 10.1021/acsnano.7b07384.
  9. Ibarra-Sánchez, L.A. et al. (2018). Antimicrobial behavior of phage endolysin PlyP100 and its synergy with nisin to control Listeria monocytogenes in Queso Fresco. Food Microbiol. 72:128-134. doi: 10.1016/j.fm.2017.11.013.
  10. Reyes-Corona, D. et al. (2017). Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion. PLoS One. 12(11):e0188239. doi: 10.1371/journal.pone.0188239.
  11. Warnock, N.D. et al. (2017). Nematode neuropeptides as transgenic nematicides. PLoS Pathog. 13(2):e1006237. doi: 10.1371/journal.ppat.1006237.
  12. Rinaldo, A. R. et al. (2015). A grapevine anthocyanin acyltransferase, transcriptionally regulated by VvMYBA, can produce most acylated anthocyanins present in grape skins. Plant Physiol. 169:1897-916.
  13. Akiyama, Y. et al. (2014). The identification of affinity peptide ligands specific to the variable region of human antibodies. Biomed Res. 35:105-116.
  14. Dong, Y. et al. (2013). HMGB1 Protein Does Not Mediate the Inflammatory Response in Spontaneous Spinal Cord Regeneration: A HINT FOR CNS REGENERATION. J. Biol. Chem. 288:18204-182128.