Lipid Droplet Isolation Kit

Lipid Droplet Isolation Kit
  • Isolates lipid droplets from cells or tissues
  • Uses a simple gradient centrifugation protocol
  • No ultracentrifugation required
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Lipid Droplet Isolation Kit
Catalog Number
MET-5011
Size
50 preps
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$360.00
Product Details

Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

Our Lipid Droplet Isolation Kit isolates lipid droplets using a simple gradient centrifugation protocol. Cells or tissues are homogenized, a gradient is created, and the samples are centrifuged. Lipid droplets float to the top and are recovered by pipetting off the top layer.

Recent Product Citations
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  2. Hara, M. et al. (2023). PNPLA2 mobilizes retinyl esters from retinosomes and promotes the generation of 11-cis-retinal in the visual cycle. Cell Rep. 42(2):112091. doi: 10.1016/j.celrep.2023.112091.
  3. Chen, W.F. (2023). AmAtg2B-Mediated Lipophagy Regulates Lipolysis of Pupae in Apis mellifera. Int J Mol Sci. 24(3):2096. doi: 10.3390/ijms24032096.
  4. Soupene, E. & Kuypers, F.A. (2022). Dual Role of ACBD6 in the Acylation Remodeling of Lipids and Proteins. Biomolecules. 12(12):1726. doi: 10.3390/biom12121726.
  5. Kieu, T.L. et al. (2022). Downregulation of Elovl5 promotes breast cancer metastasis through a lipid-droplet accumulation-mediated induction of TGF-β receptors. Cell Death Dis. 13(9):758. doi: 10.1038/s41419-022-05209-6.
  6. Ohira, H. et al. (2022). Suppression of colonic oxidative stress caused by chronic ethanol administration and attenuation of ethanol-induced colitis and gut leakiness by oral administration of sesaminol in mice. Food Funct. doi: 10.1039/d1fo04120g.
  7. Wu, S.C. et al. (2022). Stomatin modulates adipogenesis through the ERK pathway and regulates fatty acid uptake and lipid droplet growth. Nat Commun. 13(1):4174. doi: 10.1038/s41467-022-31825-z.
  8. Zhang, Z. et al. (2022). Loss of immunity-related GTPase GM4951 leads to nonalcoholic fatty liver disease without obesity. Nat Commun. 13(1):4136. doi: 10.1038/s41467-022-31812-4.
  9. Yuan, S. et al. (2021). SARS-CoV-2 exploits host DGAT and ADRP for efficient replication. Cell Discov. 7(1):100. doi: 10.1038/s41421-021-00338-2.
  10. Pu, Q. et al. (2021). Bitter receptor TAS2R138 facilitates lipid droplet degradation in neutrophils during Pseudomonas aeruginosa infection. Signal Transduct Target Ther. 6(1):210. doi: 10.1038/s41392-021-00602-7.
  11. Galano, M. et al. (2021). Role of Constitutive STAR in Leydig Cells. Int J Mol Sci. 22(4):2021. doi: 10.3390/ijms22042021.
  12. Ohira, H. et al. (2021). Alteration of oxidative-stress and related marker levels in mouse colonic tissues and fecal microbiota structures with chronic ethanol administration: Implications for the pathogenesis of ethanol-related colorectal cancer. PLoS One. 16(2):e0246580. doi: 10.1371/journal.pone.0246580.
  13. Du, X. et al. (2019). ORP5 localizes to ER-lipid droplet contacts and regulates the level of PI(4)P on lipid droplets. J Cell Biol. pii: jcb.201905162. doi: 10.1083/jcb.201905162.
  14. Sugihara, M. et al. (2019). The AAA+ ATPase/ubiquitin ligase mysterin stabilizes cytoplasmic lipid droplets. J Cell Biol. 218(3):949-960. doi: 10.1083/jcb.201712120.