Lipid Extraction Kit

Lipid Extraction Kit
  • Chloroform-free organic extraction method
  • Extract lipids from plasma, serum, or cultured cells
  • Extraction into upper organic phase is amenable to high-throughput applications

NOTE: Product #STA-612 is shipped as Dangerous Goods and cannot be ordered on our website. Please call us for details.

Email To BuyerPrint this PageCopy Link
Ordering

Please contact your distributor for pricing.

Lipid Extraction Kit (Chloroform-Free)
Catalog Number
STA-612
Size
50 preps
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$0.00
Lipid Extraction Kit (Chloroform-Free), Trial Size
Catalog Number
STA-612-T
Size
10 preps
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$195.00
Product Details

Traditional lipid extraction methods such as the Folch method rely on chloroform, which has two disadvantages: chloroform is a potential carcinogen, and extraction into chloroform results in the lipids being in the lower phase, which is inconvenient.

Our Lipid Extraction Kit eliminates both aforementioned problems by using a chloroform-free organic system that results in the organic phase being on the top. This allows easy removal of the lipid-containing layer as well as adaptation to high-throughput liquid handling systems. Lipids may be extracted from plasma or serum, or from cells grown in culture.

Recent Product Citations
  1. Seki, M. et al. (2022). Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissue. Biosci Microbiota Food Health. doi: 10.12938/bmfh.2021-061.
  2. Fukami, H. et al. (2021). Vaccine targeting ANGPTL3 ameliorates dyslipidemia and associated diseases in mouse models of obese dyslipidemia and familial hypercholesterolemia. Cell Rep Med. 2(11):100446. doi: 10.1016/j.xcrm.2021.100446.
  3. Bajaj, A. et al. (2020). Method of extraction and proteome profiling of mycobacteria using liquid chromatography-high resolution mass spectrometry. SN Appl. Sci. doi: 10.1007/s42452-020-03691-1.
  4. Jun, Y. et al. (2020). Leukocyte-Mediated Combined Targeted Chemo and Gene Therapy for Esophageal Cancer. ACS Appl Mater Interfaces. doi: 10.1021/acsami.0c15419.
  5. Greco, C.M. et al. (2020). A non-pharmacological therapeutic approach in the gut triggers distal metabolic rewiring capable of ameliorating diet-induced dysfunctions encompassed by metabolic syndrome. Sci Rep. 10(1):12915. doi: 10.1038/s41598-020-69469-y.
  6. Zhu, Q. et al. (2020). Bupivacaine inhibits angiogenesis through oxidative stress-dependent inhibition of Akt/mTOR and activation of AMPK. Fundam Clin Pharmacol. doi: 10.1111/fcp.12554.
  7. Srisomboon, Y. et al. (2020). Fungal allergen-induced IL-33 secretion involves cholesterol-dependent, VDAC-1-mediated ATP release from the airway epithelium. J Physiol. doi: 10.1113/JP279379.
  8. Srisowanna, N. et al. (2019). The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats. Acta Histochem. Cytochem. 52(4):67-75. doi:10.1267/ahc.19018.
  9. Huang, Z. et al. (2019). ALOX12 inhibition sensitizes breast cancer to chemotherapy via AMPK activation and inhibition of lipid synthesis. Biochem Biophys Res Commun. pii: S0006-291X(19)30743-0. doi: 10.1016/j.bbrc.2019.04.101.
  10. Yang, X. et al. (2018). Inhibiting 6-phosphogluconate dehydrogenase selectively targets breast cancer through AMPK activation. Clin Transl Oncol. 20(9):1145-1152. doi: 10.1007/s12094-018-1833-4.
  11. Matoba, K. et al. (2017). Adipose KLF15 Controls Lipid Handling to Adapt to Nutrient Availability. Cell Rep. 21(11):3129-3140. doi: 10.1016/j.celrep.2017.11.032.
  12. Tyszka-Czochara, M. et al. (2017). Metformin and caffeic acid regulate metabolic reprogramming in human cervical carcinoma SiHa/HTB-35 cells and augment anticancer activity of Cisplatin via cell cycle regulation. Food Chem. Toxicol. 106:260-272.
  13. Maki, T. et al. (2017). Renoprotective effect of a novel selective PPARα modulator K-877 in db/db mice: A role of diacylglycerol-protein kinase C-NAD(P)H oxidase pathway. Metabolism Clinical and Experimental. 71: 33–45.
  14. Tyszka-Czochara, M. et al. (2017). Caffeic Acid Expands Anti-Tumor Effect of Metformin in Human Metastatic Cervical Carcinoma HTB-34 Cells: Implications of AMPK Activation and Impairment of Fatty Acids De Novo Biosynthesis. Int J Mol Sci. doi: 10.3390/ijms18020462.
  15. Pamir, N. et al. (2015). Granulocyte macrophage-colony stimulating factor-dependent dendritic cells restrain lean adipose tissue expansion. J Biol Chem.  doi:10.1074/jbc.M115.645820.