Lipid Extraction Kit

Traditional lipid extraction methods such as the Folch method rely on chloroform, which has two disadvantages: chloroform is a potential carcinogen, and extraction into chloroform results in the lipids being in the lower phase, which is inconvenient.

Our Lipid Extraction Kit eliminates both aforementioned problems by using a chloroform-free organic system that results in the organic phase being on the top. This allows easy removal of the lipid-containing layer as well as adaptation to high-throughput liquid handling systems. Lipids may be extracted from plasma or serum, or from cells grown in culture.

Pamir, N. et al. (2015). Granulocyte macrophage-colony stimulating factor-dependent dendritic cells restrain lean adipose tissue expansion. J Biol Chem.  doi:10.1074/jbc.M115.645820.