Lipid Quantification Kit (unsaturated fatty acids)

Lipid Quantification Kit (unsaturated fatty acids)
  • Quantifies total unsaturated fatty acids from plasma, serum, or cultured cells
  • Sulfo-phospho-vanillin system provides quantitation in a 96-well plate
Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

Lipid Quantification Kit (Colorimetric)
Catalog Number
100 assays
Manual/Data Sheet Download
SDS Download
Product Details

Traditional lipid quantitation methods require expensive HPLC or light scattering detection equipment. Our Lipid Quantitation Kit provides a simple method for quantitation of lipids in a standard 96-well microplate reader. The kit uses a sulfo-phospho-vanillin system in which samples are acidified and heated to solubilize and prime them for quantitation. In an acidic environment, vanillin reagent reacts with the lipids to form a colored product that is easily detected by the plate reader at 540 nm.

Recent Product Citations
  1. Barrett, E. et al. (2021). Uteroplacental Insufficiency with Hypoxia Upregulates Placental PPARγ-KMT5A Axis in the Rat. Reprod Sci. doi: 10.1007/s43032-020-00434-w.
  2. Kumar, S. et al. (2021). Cocaine-Specific Effects on Exosome Biogenesis in Microglial Cells. Neurochem Res. doi: 10.1007/s11064-021-03231-2.
  3. Pishavar, E. et al. (2020). Comparison Study of Stem Cell-Derived Extracellular Vesicles for Enhanced Osteogenic Differentiation. Tissue Eng Part A. doi: 10.1089/ten.TEA.2020.0194.
  4. Kawabe, Y. et al. (2020). (Bombyx Insulin-Like Peptide) Increases the Respiration Rate Through Facilitation of Carbohydrate Catabolism in Bombyx mori. Front Endocrinol (Lausanne). 10:150. doi: 10.3389/fendo.2019.00150.
  5. Inagaki, T. et al. (2020). Gut microbial pulse provides nutrition for parental provisioning in incipient termite colonies. Behav Ecol Sociobiol. 74(6). doi: 10.1007/s00265-020-02843-y.
  6. Zhu, Q. et al. (2020). Bupivacaine inhibits angiogenesis through oxidative stress-dependent inhibition of Akt/mTOR and activation of AMPK. Fundam Clin Pharmacol. doi: 10.1111/fcp.12554.
  7. Nair, P. et al. (2020). Metabolic responses to long-term food deprivation in subterranean and surface amphipods. Subterr Biol. 33:1–15. doi: 10.3897/subtbiol.33.48483.
  8. Langridge, T.D. et al. (2019). Toward understanding polymer micelle stability: Density ultracentrifugation offers insight into polymer micelle stability in human fluids. J Control Release. 319:157-167. doi: 10.1016/j.jconrel.2019.12.038.
  9. Ahmadpour, A. et al. (2019). Study of the Circulatory Energy Balance Indicators and Hepatic Fat Content in Dromedary Camel during Late Pregnancy and Early Lactation. Small Ruminant Research. doi: 10.1016/j.smallrumres.2019.08.018.
  10. Huang, Z. et al. (2019). ALOX12 inhibition sensitizes breast cancer to chemotherapy via AMPK activation and inhibition of lipid synthesis. Biochem Biophys Res Commun. pii: S0006-291X(19)30743-0. doi: 10.1016/j.bbrc.2019.04.101.
  11. Fanning, S. et al. (2019). Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. Mol Cell. 73(5):1001-1014.e8. doi: 10.1016/j.molcel.2018.11.028.
  12. Stranska, R. et al. (2018). Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma. J Transl Med. 16(1):1. doi: 10.1186/s12967-017-1374-6.
  13. Yang, X. et al. (2018). Inhibiting 6-phosphogluconate dehydrogenase selectively targets breast cancer through AMPK activation. Clin Transl Oncol. 20(9):1145-1152. doi: 10.1007/s12094-018-1833-4.
  14. Tosic, M. et al. (2018). Lsd1 regulates skeletal muscle regeneration and directs the fate of satellite cells. Nat Commun. 9(1):366. doi: 10.1038/s41467-017-02740-5.
  15. Gupta, K.H. et al. (2017). Apoptosis and compensatory proliferation signaling are coupled by CrkI-containing microvesicles. Dev. Cell. 41(6):674-684.
  16. Tyszka-Czochara, M. et al. (2017). Caffeic Acid Expands Anti-Tumor Effect of Metformin in Human Metastatic Cervical Carcinoma HTB-34 Cells: Implications of AMPK Activation and Impairment of Fatty Acids De Novo Biosynthesis. Int J Mol Sci. doi: 10.3390/ijms.
  17. Tasaki, E. et al (2017). An Efficient Antioxidant System in a Long-Lived Termite Queen. PLoS One. 12(1):e0167412. doi: 10.1371/journal.pone.0167412.
  18. Duteil, D. et al. (2016). Lsd1 ablation triggers metabolic reprogramming of brown adipose tissue. Cell Reports 17:1008-1021.