MTT Cell Proliferation Assay

  • Colorimetric format for measuring and monitoring cell proliferation
  • Kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates
  • Cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells
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CytoSelect™ MTT Cell Proliferation Assay
Catalog Number
CBA-252
Size
960 assays in 96-well plates
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$310.00
Product Details

Cell Biolabs’ CytoSelect™ MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

Recent Product Citations
  1. Isyar, M. et al. (2016). Are we economically efficient enough to increase the potential of in vitro proliferation of osteoblasts by means of pharmacochemical agents?. Open Orthop J. 10:420-430.
  2. Kim, M. et al. (2016). Preclinical validation of a single-treatment infusion modality that can eradicate extremity melanomas. Cancer Res. 76:6620-6630.
  3. Gumustas, F. et al. (2016). Chondrocyte proliferation, viability and differentiation is declined following administration of methylphenidate utilized for the treatment of attention-deficit/hyperactivity disorder. Human and Exp. Toxicol. doi:10.1077/096032
  4. Gumustas, S. A. et al. (2016). Assessing the negative impact of phenyl alkanoic acid derivative, a frequently prescribed drug for the suppression of pain and inflammation, on the differentiation and proliferation of chondrocytes. J Orthop Surg Res. doi:10.1186/s13018-016-0406-x.
  5. Guzelant, A. Y. et al. (2016). Are chondrocytes damaged when rheumatologic inflammation is suppressed? Drug Chem Toxicol. doi:10.3109/01480545.2016.1166249.
  6. Dogan, M. et al. (2015). Are the leading drugs against Staphylococcus aureus really toxic to cartilage? J Infect Public Health. doi:10.1016/j.jiph.2015.10.004.
  7. Wu, H. et al. (2015). MicroRNA-21 is a potential link between non-alcoholic fatty liver disease and hepatocellular carcinoma via modulation of the HBP1-p53-Srebp1c pathway. Gut.  doi:10.1136/gutjnl-2014-308430.
  8. Ren, Z. et al. (2014). Anti-tumor effect of a novel soluble recombinant human endostatin: administered as a single agent or in combination with chemotherapy agents in mouse tumor models. PLoS One. 9:e107823.
  9. Zhang, L. et al. (2014). miR-125b Can Enhance Skin Tumor Initiation and Promote Malignant Progression by Repressing Differentiation and Prolonging Cell Survival. Genes Dev. 28:2532-2546.