Myeloperoxidase Chlorination Activity Assay

Myeloperoxidase Chlorination Activity Assay, Colorimetric
  • Quantifies MPO chlorination activity from whole neutrophils, neutrophil lysates, tissue homogenates, or EDTA-plasma samples
  • Read results in a standard 96-well colorimetric plate reader
  • Quantify unknown samples against a chromogen standard
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OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit, Colorimetric
Catalog Number
200 Assays
Manual/Data Sheet Download
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Product Details

Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity.

The OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit is a quantitative colorimetric assay for measuring the myeloperoxidase activity within a sample.  The MPO enzyme catalyzes the reaction of hydrogen peroxide (H2O2) with chloride ions to create hypochlorous acid (HOCl), which rapidly reacts with taurine to produce a stable taurine chloramine product.  This step readily neutralizes the HOCl, which would otherwise accumulate and inactivate MPO.  A catalase-containing stop solution is added to stop MPO catalysis by eliminating hydrogen peroxide.  Finally, taurine chloramine reacts with the yellow TNB chromogen probe, with a decrease in color indicating higher MPO activity.

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