NAD+/NADH Assays

NAD+/NADH Assays
  • Detects NAD+, NADH, or total NAD+/NADH
  • Compatible with cell or tissue lysates
  • NAD+ standard included
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NAD+/NADH Assay Kit (Colorimetric)
Catalog Number
MET-5014
Size
100 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$480.00
NAD+/NADH Assay Kit (Fluorometric)
Catalog Number
MET-5030
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$480.00
Product Details

Nicotinamide adenine dinucleotide (NAD) consists of two nucleotides, adenine and nicotinamide, connected through their phosphate groups. It is found in all living cells, where its role is to transfer electrons, such as in glycolysis and the citric acid cycle. NAD cycles between an oxidized form (NAD+), which serves as an electron acceptor, and a reduced form (NADH), which donates electrons.

Our NAD+/NADH Assay Kit detects NAD+ and NADH in cell and tissue lysates. Total NAD+/NADH can be detected or samples can be treated with an acid or base treatment to specifically detect NAD+ or NADH. This assay uses an enzymatic cycling reaction that reduces NAD+ to NADH, which then reacts with either a colorimetric or fluorometric probe. NAD+/NADH levels in unknown samples are calculated based on the provided NAD+ standard curve.

Recent Product Citations
  1. Ugamraj, H.S. et al. (2022), TNB-738, a biparatopic antibody, boosts intracellular NAD+ by inhibiting CD38 ecto-enzyme activity. MAbs. 14(1):2095949. doi: 10.1080/19420862.2022.2095949 (#MET-5014).
  2. Li, X. et al. (2022). Omarigliptin alleviates cognitive dysfunction in Streptozotocin-induced diabetic mouse. Bioengineered. 13(4):9387-9396. doi: 10.1080/21655979.2022.2055699 (#MET-5014).
  3. Veeriah, V. et al. (2022). Long-term oral administration of an HNF4α agonist prevents weight gain and hepatic steatosis by promoting increased mitochondrial mass and function. Cell Death Dis. 13(1):89. doi: 10.1038/s41419-022-04521-5 (#MET-5014).
  4. Kotla, S. et al. (2021). Nucleus-mitochondria positive feedback loop formed by ERK5 S496 phosphorylation-mediated poly (ADP-ribose) polymerase activation provokes persistent pro-inflammatory senescent phenotype and accelerates coronary atherosclerosis after chemo-radiation. Redox Biol. doi: 10.1016/j.redox.2021.102132 (#MET-5030).
  5. Yumnam, S. et al. (2020). Downregulation of dihydrolipoyl dehydrogenase by UVA suppresses melanoma progression via triggering oxidative stress and altering energy metabolism. Free Radic Biol Med. doi: 10.1016/j.freeradbiomed.2020.11.037 (#MET-5014).
  6. Wahba, N.S. et al. (2019). Vitamin D3 potentiates the renoprotective effects of vildagliptin in a rat model of fructose/salt-induced insulin resistance. Eur J Pharm Sci. doi: 10.1016/j.ejps.2019.105196 (#MET-5014).
  7. Maurer, S. et al. (2019). Investigation of fatty aldehyde and alcohol synthesis from fatty acids by αDox- or CAR-expressing Escherichia coli. J Biotechnol. pii: S0168-1656(19)30828-4. doi: 10.1016/j.jbiotec.2019.08.011 (#MET-5014).
  8. Kawaguchi, A.T. et al. (2019). Artificial Oxygen Carrier Improves Fatigue-Resistance in Slow-muscle but not in Fast-muscle in a Rat in situ Model. Artif Organs. doi: 10.1111/aor.13535 (#MET-5030).