Nitrotyrosine ELISA Kit

Nitrotyrosine ELISA Kit
  • Sensitive detection of 3-Nitrotyrosine as low as 10 nM
  • Suitable for use with cell lysates, serum, plasma and purified proteins
  • Nitrated BSA provided as a standard

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General FAQs about Oxidative Stress

Video: Color Development in an ELISA

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OxiSelect™ Nitrotyrosine ELISA Kit
Catalog Number
96 assays
Manual/Data Sheet Download
SDS Download
OxiSelect™ Nitrotyrosine ELISA Kit
Catalog Number
5 x 96 assays
Manual/Data Sheet Download
SDS Download
Product Details

Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress.

Our OxiSelect™ Nitrotyrosine ELISA Kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins.

Formation of 3-Nitrotyrosine During Oxidative Stress. An increase in the presence of nitrotyrosine is correlated with an increase in the presence of nitric oxide (NO).

Protein Nitration by Tetranitromethane using the OxiSelect™ Nitrotyrosine ELISA Kit. STO (MEF) cells were lysed in 25 mM HEPES, pH 7.5, 150 mM NaCl, 1% NP-40, 10 mM MgCl2, 1 mM EDTA, 2% glycerol. The cell lysate was nitrated with tetranitromethane. The protein 3-nitrotyrosine levels were measured as described in the assay protocol.

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