Nitrotyrosine ELISA Kit

Nitrotyrosine ELISA Kit
  • Sensitive detection of 3-Nitrotyrosine as low as 10 nM
  • Suitable for use with cell lysates, serum, plasma and purified proteins
  • Nitrated BSA provided as a standard

 

Video: Color Development in an ELISA

Email To BuyerPrint this PageCopy Link
Ordering

Please contact your distributor for pricing.

OxiSelect™ Nitrotyrosine ELISA Kit
Catalog Number
STA-305
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$675.00
OxiSelect™ Nitrotyrosine ELISA Kit
Catalog Number
STA-305-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$2,885.00
OxiSelect™ Nitrotyrosine ELISA Kit, Trial Size
Catalog Number
STA-305-T
Size
32 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$330.00
Product Details

Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress.

Our OxiSelect™ Nitrotyrosine ELISA Kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins.

Formation of 3-Nitrotyrosine During Oxidative Stress. An increase in the presence of nitrotyrosine is correlated with an increase in the presence of nitric oxide (NO).

Protein Nitration by Tetranitromethane using the OxiSelect™ Nitrotyrosine ELISA Kit. STO (MEF) cells were lysed in 25 mM HEPES, pH 7.5, 150 mM NaCl, 1% NP-40, 10 mM MgCl2, 1 mM EDTA, 2% glycerol. The cell lysate was nitrated with tetranitromethane. The protein 3-nitrotyrosine levels were measured as described in the assay protocol.

Recent Product Citations
  1. Dietrich-Muszalska, A. et al. (2021). Comparative Study of the Effects of Atypical Antipsychotic Drugs on Plasma and Urine Biomarkers of Oxidative Stress in Schizophrenic Patients. Neuropsychiatr Dis Treat. 17:555-565. doi: 10.2147/NDT.S283395.
  2. Zhou, Q. et al. (2021). Physiological Responses of Microcystis aeruginosa to Extracellular Degradative Enzymes and Algicidal Substance from Heterotrophic Bacteria. Pol. J. Environ. Stud. 30(3): 1-9. doi: 10.15244/pjoes/127867.
  3. Ohira, H. et al. (2021). Alteration of oxidative-stress and related marker levels in mouse colonic tissues and fecal microbiota structures with chronic ethanol administration: Implications for the pathogenesis of ethanol-related colorectal cancer. PLoS One. 16(2):e0246580. doi: 10.1371/journal.pone.0246580.
  4. Uzunköprü, C. et al. (2021). Retinal Nerve Fiber Layer Thickness Correlates with Serum and Cerebrospinal Fluid Neurofilament Levels and is Associated with Current Disability in Multiple Sclerosis. Arch Neuropsychitry. doi: 10.29399/npa.27355.
  5. Gerszi, D. et al. (2021). Evaluation of oxidative/nitrative stress and uterine artery pulsatility index in early pregnancy. Physiol Int. doi: 10.1556/2060.2020.00041.
  6. Gil, A. et al. (2020). Neuronal Metabolism and Neuroprotection: Neuroprotective Effect of Fingolimod on Menadione-Induced Mitochondrial Damage. Cells. 10(1):E34. doi: 10.3390/cells10010034.
  7. Miller, V.J. et al. (2020). A Ketogenic Diet Combined With Exercise Alters Mitochondrial Function In Human Skeletal Muscle While Improving Metabolic Health. Am J Physiol Endocrinol Metab. doi: 10.1152/ajpendo.00305.2020.
  8. Sprick, J.D. et al. (2020). Augmented exercise pressor response during maximal treadmill exercise is not related to systemic inflammation in stroke survivors. Top Stroke Rehabil. doi: 10.1080/10749357.2020.1806436.
  9. Hamidi, V. et al. (2020). Acute Exenatide Therapy Attenuates Postprandial Vasodilation in Humans with Prediabetes: A Randomized Controlled Trial. Metab Syndr Relat Disord. doi: 10.1089/met.2019.0102.
  10. Valvassori, S.S. et al. (2020). Protein kinase C isoforms as a target for manic-like behaviors and oxidative stress in a dopaminergic animal model of mania. Prog Neuropsychopharmacol Biol Psychiatry. doi: 10.1016/j.pnpbp.2020.109940.
  11. Vujacic-Mirski, K. et al. (2020). Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological Samples and Comparison with Classical, Antibody-Based Methods. Antioxidants (Basel). 9(5). pii: E388. doi: 10.3390/antiox9050388.
  12. Chira, A. et al. (2020). Monocyte chemotactic protein‑1 and nitrotyrosine in irritable bowel syndrome. Exp Ther Med. doi: 10.3892/etm.2020.8665.
  13. Casós, K. et al. (2020). Determination of Redox Status in Serum. Methods Mol Biol. 2110:115-128. doi: 10.1007/978-1-0716-0255-3_8.
  14. Ruottinen, M. et al. (2020). The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer. Anticancer Res. 40(1):253-259. doi: 10.21873/anticanres.13947.
  15. Menegas, S. et al. (2020). Efficacy of folic acid as an adjunct to lithium therapy on manic-like behaviors, oxidative stress and inflammatory parameters in an animal model of mania. Metab Brain Dis. doi: 10.1007/s11011-019-00503-3.
  16. Mikolka, P. et al. (2019). Effect of different dosages of dexamethasone therapy on lung function and inflammation in an early phase of acute respiratory distress syndrome model. Physiol Res. 68(Supplementum 3):S253-S263. doi: 10.33549/physiolres.934364.
  17. Balasubramanian, P. et al. (2019). Obesity-Induced Sympathoexcitation is Associated with Nrf2 Dysfunction in the Rostral VentroLateral Medulla. Am J Physiol Regul Integr Comp Physiol. doi: 10.1152/ajpregu.00206.2019.
  18. Streese, L. et al. (2019). Physical activity may drive healthy microvascular ageing via downregulation of p66Shc. Eur J Prev Cardiol. doi: 10.1177/2047487319880367.
  19. Chazarin, B. et al. (2019). Limited Oxidative Stress Favors Resistance to Skeletal Muscle Atrophy in Hibernating Brown Bears (Ursus Arctos). Antioxidants (Basel). 8(9). pii: E334. doi: 10.3390/antiox8090334.
  20. Suvakov, S. et al. (2019). Markers of Oxidative Stress and Endothelial Dysfunction Predict Haemodialysis Patients Survival. Am J Nephrol. doi: 10.1159/000501300.
  21. Jerotic, D. et al. (2019). Association of Nrf2, SOD2 and GPX1 Polymorphisms with Biomarkers of Oxidative Distress and Survival in End-Stage Renal Disease Patients. Toxins (Basel). 11(7). pii: E431. doi: 10.3390/toxins11070431.
  22. Simenauer, A. et al. (2019). Repression of Nrf2/ARE regulated antioxidant genes and dysregulation of the cellular redox environment by the HIV Transactivator of Transcription. Free Radic Biol Med. 141:244-252. doi: 10.1016/j.freeradbiomed.2019.06.015.
  23. Tucker, M.A. et al. (2019). Endothelial Dysfunction in Cystic Fibrosis: Role of Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2019:1-8. doi: 10.1155/2019/1629638.
  24. Erlandsson, L. et al. (2019). Alpha-1 microglobulin as a potential therapeutic candidate for treatment of hypertension and oxidative stress in the STOX1 preeclampsia mouse model. Sci Rep. 9(1):8561. doi: 10.1038/s41598-019-44639-9.
  25. Wan, P. et al. (2019). LncRNA H19 initiates microglial pyroptosis and neuronal death in retinal ischemia/reperfusion injury. Cell Death Differ. doi: 10.1038/s41418-019-0351-4.
  26. Tarantini, S. et al. (2019). Nicotinamide mononucleotide (NMN) supplementation rescues cerebromicrovascular endothelial function and neurovascular coupling responses and improves cognitive function in aged mice. Redox Biol. 24:101192. doi: 10.1016/j.redox.2019.101192.
  27. Dal-Pont, G.C. et al. (2019). Tamoxifen has an anti-manic effect but not protect the brain against oxidative stress in an animal model of mania induced by ouabain. J Psychiatr Res. 113:181-189. doi: 10.1016/j.jpsychires.2019.03.020.
  28. Menegas, S. et al. (2019). Resveratrol protects the brain against oxidative damage in a dopaminergic animal model of mania. Metab Brain Dis. doi: 10.1007/s11011-019-00408-1.
  29. Kuosmanen, V. et al. (2019). Rectus Sheath Block (RSB) Analgesia Could Enhance Significantly the Patient Satisfaction Following Midline Laparotomy in Benign Disease and in Cancer: A Prospective Study With Special Reference to Nitrosative Stress Marker Nitrotyrosine (NT) Plasma Concentrations. Anticancer Res. 39(3):1383-1389. doi: 10.21873/anticanres.13252.
  30. Scordo, J.M. et al. (2019). The human lung mucosa drives differential Mycobacterium tuberculosis infection outcome in the alveolar epithelium. Mucosal Immunol. 12(3):795-804. doi: 10.1038/s41385-019-0156-2.