Cell Biolabs’ Zinc Assay Kit is a simple colorimetric assay that measures the total amount of zinc present in biological samples in a 96-well microtiter plate format. Samples are deproteinized to release bound zinc. Free zinc forms a complex with the color reagent to produce a bright red color, which is then measured with a standard 96-well spectrophotometric microtiter plate reader. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, zinc standards, and unknown samples. Sample zinc concentrations are determined by comparison with a known zinc standard. The kit has a detection sensitivity limit of ~0.4 µM zinc.