Radius™ 96-Well Cell Migration Assay

  • Proprietary biocompatible hydrogel creates a circular area across which cells may migrate following gel removal
  • Versatile plate format allows use with cells of any size; no need to worry about selecting cell culture inserts with the proper pore size
  • Allows qualitative, quantitative, endpoint or real-time analysis
  • Adaptable to liquid handling equipment and HCS instrumentation


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Radius™ 96-Well Cell Migration Assay
Catalog Number
CBA-126
Size
96 assays
Detection
Microscopy
Manual/Data Sheet Download
SDS Download
Price
$450.00
Radius™ 96-Well Cell Migration Assay
Catalog Number
CBA-126-5
Size
5 x 96 assays
Detection
Microscopy
Manual/Data Sheet Download
SDS Download
Price
$1,895.00
Product Details

The Radius™ Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius™ assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius™ gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius™ gel, creating a cell-free zone. Following cell seeding the Radius™ gel is removed, allowing migratory cells to move across the area and close the gap.

Assay Principle.

Various Detection Methods with Radius™ Cell Migration Assay. HeLa cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were stained according to the assay protocol with Cell Stain Solution, Calcein AM (not included in kit), or DAPI.

Cell Migration Time Course. HeLa, HT1080 and NIH3T3 cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were allowed to migrate for the various times shown.

Inhibition of HeLa Cell Migration by Cytochalasin D. HeLa cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were allowed to migrate for 24 hours in the presence of various concentrations of Cytochalasin D.

Recent Product Citations
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  2. Matijevic Glavan, T. et al. (2016). Toll-like receptor 3 stimulation triggers metabolic reprogramming in pharyngeal cancer cell ine through Myc, MAPK and HIF. Mol. Carcinog. doi:10.1002/mc.22584.
  3. Inoue, K. et al. (2016). Expression of hedgehog signals and growth inhibition by itraconazole in endometrial cancer. Anticancer Res.36:149-153.
  4. Tsubamoto, H. et al. (2016). Gremlin 2 is repressed in invasive endometrial cancer and inhibits cell growth in vitro. Anticancer Res. 36:199-203.
  5. Camacho, M. et al. (2015). Prostacyclin-synthase expression in head and neck carcinoma patients and its prognostic value in the response to radiotherapy. J Pathol. 235:125-135.
  6. Woodard, G. E. et al. (2014). Characterization of discrete subpopulations of progenitor cells in traumatic human extremity wounds. PLoS One.  9:e114318.
  7. Felthaus, O. et al. (2014). Migration of human dental follicle cells in vitro. J Periodontal Res. 49:205-212.
  8. Wong, B. et al. (2013). Adrenomedullin Enhances Invasion of Human Extravillous Cytotrophoblast-Derived Cell Lines by Regulation of Urokinase Plasminogen Activator Expression and S-Nitrosylation. Biol Reprod. 88:34 (#CBA-126).
  9. Ichikawa, A. et al. (2013). CXCL10-CXCR3 Enhances the Development of Neutrophil-mediated Fulminant Lung Injury of Viral and Nonviral Origin. Am. J. Respir. Crit. Care. Med. 187:65-77 (#CBA-126).
  10. Coulouarn, C. et al. (2012). Hepatocyte–Stellate Cell Cross-Talk in the Liver Engenders a Permissive Inflammatory Microenvironment That Drives Progression in Hepatocellular Carcinoma. Cancer Res. 72: 2533-2542 (#CBA-126). 
  11. Alcolea, S. et al. (2012).Interaction Between Head and Neck Squamous Cell Carcinoma Cells and Fibroblasts in the Biosynthesis of PGE2. J.Lipid Res. 53:630-642(#CBA-126).