Radius™ 96-Well Cell Migration Assay

Radius™ 96-Well Cell Migration Assay
  • Proprietary biocompatible hydrogel creates a circular area across which cells may migrate following gel removal
  • Versatile plate format allows use with cells of any size; no need to worry about selecting cell culture inserts with the proper pore size
  • Allows qualitative, quantitative, endpoint or real-time analysis
  • Adaptable to liquid handling equipment and HCS instrumentation

Software Analysis Tools for use with this product

Frequently Asked Questions about this product

Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

Radius™ 96-Well Cell Migration Assay
Catalog Number
96 assays
Manual/Data Sheet Download
SDS Download
Radius™ 96-Well Cell Migration Assay
Catalog Number
5 x 96 assays
Manual/Data Sheet Download
SDS Download
Product Details

The Radius™ Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius™ assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius™ gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius™ gel, creating a cell-free zone. Following cell seeding the Radius™ gel is removed, allowing migratory cells to move across the area and close the gap.

Assay Principle.

Various Detection Methods with Radius™ Cell Migration Assay. HeLa cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were stained according to the assay protocol with Cell Stain Solution, Calcein AM (not included in kit), or DAPI.

Cell Migration Time Course. HeLa, HT1080 and NIH3T3 cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were allowed to migrate for the various times shown.

Inhibition of HeLa Cell Migration by Cytochalasin D. HeLa cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were allowed to migrate for 24 hours in the presence of various concentrations of Cytochalasin D.

Recent Product Citations
  1. Pearson, P. et al. (2023). Kruppel-family zinc finger proteins as emerging epigenetic biomarkers in head and neck squamous cell carcinoma. J Otolaryngol Head Neck Surg. 52(1):41. doi: 10.1186/s40463-023-00640-x.
  2. Shin, M.D. et al. (2022). Multivalent Display of ApoAI Peptides on the Surface of Tobacco Mosaic Virus Nanotubes Improves Cholesterol Efflux. Bioconjug Chem. doi: 10.1021/acs.bioconjchem.2c00371.
  3. Li, L. et al. (2022). Trehalose Protects Keratinocytes against Ultraviolet B Radiation by Activating Autophagy via Regulating TIMP3 and ATG9A. Oxid Med Cell Longev. doi: 10.1155/2022/9366494.
  4. Wolcott, K.M. et al. (2020). CD34 positive cells isolated from traumatized human skeletal muscle require the CD34 protein for multi-potential differentiation. Cell Signal. doi: 10.1016/j.cellsig.2020.109711.
  5. Toyohara, T. et al. (2020). Patient hiPSCs Identify Vascular Smooth Muscle Arylacetamide Deacetylase as Protective against Atherosclerosis. Cell Stem Cell. S1934-5909(20)30157-0. doi: 10.1016/j.stem.2020.04.018.
  6. Ali, R. et al. (2019). PARP1 blockade is synthetically lethal in XRCC1 deficient sporadic epithelial ovarian cancers. Cancer Lett. pii: S0304-3835(19)30538-5. doi: 10.1016/j.canlet.2019.10.035.
  7. Sapp, R.M. et al. (2019). Circulating microRNAs and endothelial cell migration rate are associated with metabolic syndrome and fitness level in postmenopausal African American women. Physiol Rep. 7(14):e14173. doi: 10.14814/phy2.14173.
  8. Iyer, D. et al. (2018). Coronary artery disease genes SMAD3 and TCF21 promote opposing interactive genetic programs that regulate smooth muscle cell differentiation and disease risk. PLoS Genet. 14(10):e1007681. doi: 10.1371/journal.pgen.1007681.
  9. Fraineau, S. et al. (2017). Epigenetic Activation of Pro-angiogenic Signaling Pathways in Human Endothelial Progenitors Increases Vasculogenesis. Stem Cell Reports. 9(5):1573-1587. doi: 10.1016/j.stemcr.2017.09.009.
  10. Wei, W.C. et al. (2017). Functional expression of calcium-permeable Canonical Transient Receptor Potential 4-containing channels promotes migration of medulloblastoma cells. J. Physiol. doi:10.1113/JP274659.
  11. Sidthipong, K. et al. (2016). Rational design, synthesis and in vitro evaluation of novel exo-methylene butyrolactone salicyloylamide as NF-kB inhibitor. Bioorg. Med. Chem. Lett. doi:10.1016/j.bmcl.2016.12.017.
  12. Matijevic Glavan, T. et al. (2016). Toll-like receptor 3 stimulation triggers metabolic reprogramming in pharyngeal cancer cell ine through Myc, MAPK and HIF. Mol. Carcinog. doi:10.1002/mc.22584.
  13. Inoue, K. et al. (2016). Expression of hedgehog signals and growth inhibition by itraconazole in endometrial cancer. Anticancer Res.36:149-153.
  14. Tsubamoto, H. et al. (2016). Gremlin 2 is repressed in invasive endometrial cancer and inhibits cell growth in vitro. Anticancer Res. 36:199-203.
  15. Camacho, M. et al. (2015). Prostacyclin-synthase expression in head and neck carcinoma patients and its prognostic value in the response to radiotherapy. J Pathol. 235:125-135.
  16. Woodard, G. E. et al. (2014). Characterization of discrete subpopulations of progenitor cells in traumatic human extremity wounds. PLoS One.  9:e114318.
  17. Felthaus, O. et al. (2014). Migration of human dental follicle cells in vitro. J Periodontal Res. 49:205-212.
  18. Wong, B. et al. (2013). Adrenomedullin Enhances Invasion of Human Extravillous Cytotrophoblast-Derived Cell Lines by Regulation of Urokinase Plasminogen Activator Expression and S-Nitrosylation. Biol Reprod. 88:34.
  19. Ichikawa, A. et al. (2013). CXCL10-CXCR3 Enhances the Development of Neutrophil-mediated Fulminant Lung Injury of Viral and Nonviral Origin. Am. J. Respir. Crit. Care. Med. 187:65-77.
  20. Coulouarn, C. et al. (2012). Hepatocyte–Stellate Cell Cross-Talk in the Liver Engenders a Permissive Inflammatory Microenvironment That Drives Progression in Hepatocellular Carcinoma. Cancer Res. 72: 2533-2542. 
  21. Alcolea, S. et al. (2012).Interaction Between Head and Neck Squamous Cell Carcinoma Cells and Fibroblasts in the Biosynthesis of PGE2. J.Lipid Res. 53:630-642.