Ras Activation Assays

  • Safe non-radioactive assay format
  • Colored agarose beads allow visual check
  • Fast results: 1 hour plus electrophoresis/blotting time
  • Compatible with human, mouse, and rat samples

 

Frequently Asked Questions about this product

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H-Ras Activation Assay Kit
Catalog Number
STA-400-H
Size
20 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$610.00
K-Ras Activation Assay Kit
Catalog Number
STA-400-K
Size
20 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$610.00
N-Ras Activation Assay Kit
Catalog Number
STA-400-N
Size
20 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$610.00
Pan-Ras Activation Assay Kit, Trial Size
Catalog Number
STA-400-T
Size
5 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$305.00
H-Ras Activation Assay Kit, Trial Size
Catalog Number
STA-400-H-T
Size
5 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$305.00
K-Ras Activation Assay Kit, Trial Size
Catalog Number
STA-400-K-T
Size
5 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$305.00
N-Ras Activation Assay Kit, Trial Size
Catalog Number
STA-400-N-T
Size
5 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$305.00
Pan-Ras Activation Assay Kit
Catalog Number
STA-400
Size
20 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$610.00
Product Details

Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit.

Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

Small GTPase Activation Assay Principle

Recent Product Citations
  1. Nakaoka, H. J. et al. (2016). NECAB3 promotes activation of hypoxia-inducible factor-1 during normoxia and enhances tumourigenicity of cancer cells. Sci Rep.6:22784.
  2. Krencik, R. et al. (2015). Dysregulation of astrocyte extracellular signaling in Costello syndrome.  Sci Transl Med. doi:10.1126/scitranslmed.aaa5645.
  3. Sun, Y. et al. (2014). Reduced miR-3127-5p expression promotes NSCLC proliferation/invasion and contributes to dasatinib sensitivity via the c-Abl/Ras/ERK pathway. Sci Rep. 4:6527.
  4. Hernández-Porras, I. et al. (2014).  K-RasV14I Recapitulates Noonan Syndrome in Mice. Proc Natl Acad Sci U S A. 111:16395-16400.
  5. Gall, J. M. et al. (2014). Conditional Knockout of Proximal Tubule Mitofusin 2 Accelerates Recovery and Improves Survival after Renal Ischemia. J Am Soc Nephrol. 10.1681/ASN.2014010126.
  6. Moran, D. M. et al. (2014).  KRAS Mutation Status Is Associated with Enhanced Dependency on Folate Metabolism Pathways in Non–Small Cell Lung Cancer Cells. Mol Cancer Ther. 13:1611-1624.
  7. Nikolos, F. et al. (2014).  ERβ Regulates NSCLC Phenotypes by Controlling Oncogenic RAS Signaling. Mol Cancer Res. 12:843-854.
  8. Ohashi, K. et al. (2012).  Lung Cancers with Acquired Resistance to EGFR Inhibitors Occasionally Harbor BRAF Gene Mutations but Lack Mutations in KRAS, NRAS, or MEK1. PNAS. 109:E2127-E2133 (#STA-400).
  9. Geryk-Hall, M. et al. (2010). Driven to Death: Inhibition of Farnesylation Increases Ras Activity in Osteosarcoma and Promotes Growth Arrest and Cell Death. Mol. Cancer Ther. 9:1111-119 (#STA-400).
  10. Camalier, C.E. et al. (2010). Elevated Phosphate Activates N-ras and Promotes Cell Transformation and Skin Tumorigenesis. Cancer Prev. Res. 3:359-370 (#STA-400).
  11. Harmon, B. et al. (2008). Induction of the G-alpha-q Signaling Cascade by the Human Immunodeficiency Virus Envelope is Required for Virus Entry. J. Virol. 82:9191-9205 (#STA-400).