Rho Activation Assays

Rho Activation Assays
  • Safe non-radioactive assay format
  • Colored agarose beads allow visual check
  • Fast results: 1 hour plus electrophoresis/blotting time
  • Compatible with human, mouse, and rat samples

 

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RhoA Activation Assay
Catalog Number
STA-403-A
Size
20 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$640.00
RhoA/Rac1/Cdc42 Activation Assay Combo Kit
Catalog Number
STA-405
Size
3 x 10 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$940.00
Product Details

Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

If you are also studying Rac1 and/or Cdc42, you may consider our economical combination kit.

Small GTPase Activation Assay Principle

Recent Product Citations
  1. Pan, W. et al. (2021). The Regulatory Subunit PPP2R2A of PP2A Enhances Th1 and Th17 Differentiation through Activation of the GEF-H1/RhoA/ROCK Signaling Pathway. J Immunol. doi: 10.4049/jimmunol.2001266 (#STA-403-A).
  2. Mossa, A.H. et al. (2021). Deleterious impact of nerve growth factor precursor (proNGF) on bladder urothelial and smooth muscle cells. Cell Signal. doi: 10.1016/j.cellsig.2021.109936 (#STA-403-A).
  3. Jaafar, L. et al. (2021). StarD13 differentially regulates migration and invasion in prostate cancer cells. Hum Cell. doi: 10.1007/s13577-020-00479-8 (#STA-405).
  4. Kim, J.H. et al. (2021). Gamma subunit of complement component 8 is a neuroinflammation inhibitor. Brain. doi: 10.1093/brain/awaa425 (#STA-403-A).
  5. Ghassibe-Sabbagh, M. et al. (2021). Altered regulation of cell migration in IRF6-mutated orofacial cleft patients-derived primary cells reveals a novel role of Rho GTPases in cleft/lip palate development. Cells Dev. doi: 10.1016/j.cdev.2021.203674 (#STA-405).
  6. Li, M.J. et al. (2021). Ezrin Promotes the Proliferation, Migration, and Invasion of Ovarian Cancer Cells. Biomed. Environ. Sci. 34(2):139-151. doi: 10.3967/bes2021.020 (#STA-405).
  7. Xu, J. et al. (2021). Acute glucose influx-induced mitochondrial hyperpolarization inactivates myosin phosphatase as a novel mechanism of vascular smooth muscle contraction. Cell Death Dis. 12(2):176. doi: 10.1038/s41419-021-03462-9 (#STA-403-A).
  8. Matarrese, P. et al. (2021). Physical Interaction between HPV16E7 and the Actin-Binding Protein Gelsolin Regulates Epithelial-Mesenchymal Transition via HIPPO-YAP Axis. Cancers (Basel). 13(2):353. doi: 10.3390/cancers13020353 (#STA-405).
  9. El-Mais, N. et al. (2020). Human recombinant arginase I [HuArgI (Co)-PEG5000]-induced arginine depletion inhibits ovarian cancer cell adhesion and migration through autophagy-mediated inhibition of RhoA. J Ovarian Res. 14(1):13. doi: 10.1186/s13048-021-00767-3 (#STA-405).
  10. El‑Chami, D. et al. (2021). Recombinant anthrax lethal toxin inhibits cell motility and invasion in breast cancer cells through the dysregulation of Rho GTPases. Oncol Lett. 21(2):1-7. doi:  10.3892/ol.2020.12424 (#STA-405).
  11. Al Haddad, M. et al. (2020). Differential regulation of rho GTPases during lung adenocarcinoma migration and invasion reveals a novel role of the tumor suppressor StarD13 in invadopodia regulation. Cell Commun Signal. 18(1):144. doi: 10.1186/s12964-020-00635-5 (#STA-405).
  12. Wang, F. et al. (2020). Topical administration of rapamycin promotes retinal ganglion cell survival and reduces intraocular pressure in a rat glaucoma model. Eur J Pharmacol. doi: 10.1016/j.ejphar.2020.173369 (#STA-403-A).
  13. Badaoui, M. et al. (2020). Vav3 Mediates Pseudomonas aeruginosa Adhesion to the Cystic Fibrosis Airway Epithelium. Cell Rep. 32(1):107842. doi: 10.1016/j.celrep.2020.107842 (#STA-405).
  14. Rajakylä, E.K. et al. (2020). Assembly of Peripheral Actomyosin Bundles in Epithelial Cells Is Dependent on the CaMKK2/AMPK Pathway. Cell Rep. 30(12):4266-4280.e4. doi: 10.1016/j.celrep.2020.02.096 (#STA-405).
  15. Isaksen, T.J. et al. (2020). Repulsive Guidance Molecule A Suppresses Adult Neurogenesis. Stem Cell Reports. pii: S2213-6711(20)30093-X. doi: 10.1016/j.stemcr.2020.03.003 (#STA-403-A).
  16. Buonpane, C. et al. (2020). ROCK1 Inhibitor Stabilizes E-cadherin and Improves Barrier Function in Experimental Necrotizing Enterocolitis. Am J Physiol Gastrointest Liver Physiol. doi: 10.1152/ajpgi.00195.2019 (#STA-403-A).
  17. Phung, B. et al. (2019). The X-Linked DDX3X RNA Helicase Dictates Translation Reprogramming and Metastasis in Melanoma. Cell Rep. 27(12):3573-3586.e7. doi: 10.1016/j.celrep.2019.05.069 (#STA-403-A).
  18. Yoon, S. et al. (2019). EPHB6 mutation induces cell adhesion-mediated paclitaxel resistance via EPHA2 and CDH11 expression. Exp Mol Med. 51(6):61. doi: 10.1038/s12276-019-0261-z (#STA-403-A).
  19. Gao, S. et al. (2019). Histidine-rich glycoprotein ameliorates endothelial barrier dysfunction through regulation of NF-κB and MAPK signal pathway. Br J Pharmacol. doi: 10.1111/bph.14711 (#STA-403-A).
  20. Kang, M. et al. (2019). Roles of CD133 in microvesicle formation and oncoprotein trafficking in colon cancer. FASEB J. 33(3):4248-4260. doi: 10.1096/fj.201802018R (#STA-403-A).
  21. El Atat, O. et al. (2019). RHOG Activates RAC1 through CDC42 Leading to Tube Formation in Vascular Endothelial Cells. Cells. 8(2). pii: E171. doi: 10.3390/cells8020171 (#STA-405).
  22. Kim, D. et al. (2019). Lysophosphatidic acid increases mesangial cell proliferation in models of diabetic nephropathy via Rac1/MAPK/KLF5 signaling. Exp Mol Med. 51(2):18. doi: 10.1038/s12276-019-0217-3 (#STA-405).
  23. Soliman, M. et al. (2018). Rotavirus-Induced Early Activation of the RhoA/ROCK/MLC Signaling Pathway Mediates the Disruption of Tight Junctions in Polarized MDCK Cells. Sci Rep. 8(1):13931. doi: 10.1038/s41598-018-32352-y (#STA-403-A).
  24. Sorrentino, S. et al. (2018). Hindlimb Ischemia Impairs Endothelial Recovery and Increases Neointimal Proliferation in the Carotid Artery. Sci Rep. 8(1):761. doi: 10.1038/s41598-017-19136-6 (#STA-403-A).
  25. Huang, X. et al. (2018). RhoA-stimulated intra-capillary morphology switch facilitates the arrest of individual circulating tumor cells. Int J Cancer. 142(10):2094-2105. doi: 10.1002/ijc.31238 (#STA-403-A).
  26. Zhang, F. et al. (2018). GAP43, a novel metastasis promoter in non-small cell lung cancer. J Transl Med. 16(1):310. doi: 10.1186/s12967-018-1682-5 (#STA-405).
  27. Chen, Z.S. et al. (2018). Planar cell polarity gene Fuz triggers apoptosis in neurodegenerative disease models. EMBO Rep. 19(9). pii: e45409. doi: 10.15252/embr.201745409 (#STA-405).
  28. Schiapparelli, P. et al. (2017). NKCC1 regulates migration ability of glioblastoma cells by modulation of actin dynamics and interacting with cofilin. EBioMedicine. doi: 10.1016/j.ebiom.2017.06.020 (#STA-405).
  29. Ye, Y. et al. (2016). Down-regulation of 14-3-3 Zeta Inhibits TGF-β1-Induced Actomyosin Contraction in Human Trabecular Meshwork Cells Through RhoA Signaling Pathway. Invest Ophthalmol Vis Sci. doi: 10.1167/iovs.15-17438 (#STA-403-A).