AAV-DJ/8 Packaging System

AAV-DJ/8 Packaging System
  • pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
  • Packaging System contains packaging plasmids and GFP control vector; co-transfect with separate AAV expression vector containing your gene of interest
  • AAV-DJ/8 is a mutant of AAV-DJ designed to provide increased uptake in brain tissue, closely mimicking AAV-8 and AAV-9


NOTE: AAV-DJ and AAV-DJ/8 Helper Free Systems are available for sale to academic, government and non-profit research laboratories. All other purchasers require a commercial license for all fields including research use. Please contact our Business Development department for license information.


Frequently Asked Questions about AAV Expression and Packaging

General FAQs about using AAV

General FAQs about Viral Gene Delivery

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AAV-DJ/8 Helper Free Packaging System
Catalog Number
1 kit
Manual/Data Sheet Download
SDS Download
Map Download
Sequence Download
Product Details

The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

Viral Gene Delivery using the AAV Helper Free System.

Production and Transduction of AAV2-GFP. Top Left: 293AAV cells prior to transfection (10X). Top Right: 293AAV cells 48 hours after transfection (10X). Bottom Left: GFP expression in 293AAV cells 48 hours after transfection (10X). Bottom Right: GFP expression in 293AD cells 48 hours after infection (20X).

Recent Product Citations
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  3. Sánchez-Sarasúa, S. et al. (2020). AAV delivery of shRNA against IRS1 in GABAergic neurons in rat hippocampus impairs spatial memory in females and male rats. Brain Struct Funct. doi: 10.1007/s00429-020-02155-x.
  4. Dubrovsky, L. et al. (2020). Inhibition of HIV Replication by Apolipoprotein A-I Binding Protein Targeting the Lipid Rafts. mBio. 11(1). pii: e02956-19. doi: 10.1128/mBio.02956-19.
  5. Zou, T. et al. (2018). MicroRNA-410-5p exacerbates high-fat diet-induced cardiac remodeling in mice in an endocrine fashion. Sci Rep. 8(1):8780. doi: 10.1038/s41598-018-26646-4.
  6. Gong, Z. et al. (2018). Dnmt3a in the dorsal dentate gyrus is a key regulator of fear renewal. Sci Rep. 8(1):5093. doi: 10.1038/s41598-018-23533-w.
  7. Wang, P. et al. (2017). Tau interactome mapping based identification of Otub1 as Tau deubiquitinase involved in accumulation of pathological Tau forms in vitro and in vivo. Acta Neuropathol. doi:10.1007/s00401-016-1663-9.
  8. Luo, W. et al. (2016). Supernova: A versatile vector system for single-cell labeling and gene function studies in vivo. Sci. Reports 6:35747.
  9. Iwata, R. et al. (2015). Developmental RacGAP α2-chimaerin signaling is a determinant of the morphological features of dendritic spines in adulthood. J Neurosci. 35:13728-13744.