AAV-DJ Packaging System

  • pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
  • Packaging System contains packaging plasmids and GFP control vector; co-transfect with separate AAV expression vector containing your gene of interest
  • AAV-DJ system provides a hybrid capsid created from 8 different AAV serotypes to provide substantially higher infectivity rates across a broad range of tissues

 

NOTE: AAV-DJ and AAV-DJ/8 Helper Free Systems are available for sale to academic, government and non-profit research laboratories. All other purchasers require a commercial license for all fields including research use. Please contact our Business Development department for license information.

 

Frequently Asked Questions about AAV Expression and Packaging

General FAQs about using AAV

General FAQs about Viral Gene Delivery

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AAV-DJ Helper Free Packaging System
Catalog Number
VPK-400-DJ
Size
1 kit
Detection
N/A
Manual/Data Sheet Download
SDS Download
Map Download
Sequence Download
Price
$795.00
Product Details

The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

Viral Gene Delivery using the AAV Helper Free System.

Generation of AAV-DJ through Capsid DNA Family Shuffling.

Recent Product Citations
  1. Suzuki, K. et al. (2016). Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nature Commun. 7:13718.
  2. Inutsuka, A. et al. (2016). The integrative role of orexin/hypocretin neurons in nociceptive perception and analgesic regulation. Sci Rep. doi:10.1038/srep29480.
  3. Yokoi, N. et al. (2016). Identification of PSD-95 depalmitoylating enzymes. J Neurosci. 36:6431-6444.
  4. Liu, J. & Moon, Y. A. (2016). Simple purification of Adeno-Associated Virus-DJ for liver-specific gene expression. Yonsei Med J. 57:790-794.
  5. Aoki, M. et al. (2016). Involvement of 5-HT2CR RNA editing in accumbal NPY expression and behavioral despair. Eur J Neurosci. doi:10.1111/ejn.13233.