DNA Double-Strand Break Assay

DNA Double-Strand Break Assay
  • Detects phosphorylation of histone H2A.X
  • See immunofluorescence in about 3 hours
  • DNA double-strand break inducer included
Email To BuyerPrint this PageCopy Link
Ordering

Please contact your distributor for pricing.

OxiSelect™ DNA Double-Strand Break (DSB) Staining Kit
Catalog Number
STA-321
Size
100 assays
Detection
Immunofluorescence
Manual/Data Sheet Download
SDS Download
Price
$540.00
OxiSelect™ DNA Double Strand Break (DSB) Staining Kit, Trial Size
Catalog Number
STA-321-T
Size
20 assays
Detection
Immunofluorescence
Manual/Data Sheet Download
SDS Download
Price
$265.00
Product Details

Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites.

The OxiSelect™ DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.

DNA Double-Strand Break Formation in A549 Cells. A549 cells were seeded at 50,000 cells/well overnight. (A) Untreated cells. (B) Cells treated with 100 µM Etoposide for 1 hour. Immunofluorescence staining was then performed according to the Assay Protocol.

Recent Product Citations
  1. Yuan, Y. et al. (2020). Deterioration of hematopoietic autophagy is linked to osteoporosis. Aging Cell. doi: 10.1111/acel.13114.
  2. Han, S. et al. (2019). Secretome analysis of patient-derived GBM tumor spheres identifies midkine as a potent therapeutic target. Exp Mol Med. 51(12):147. doi: 10.1038/s12276-019-0351-y.
  3. Zhang, Y. et al. (2019). N-Acetyl Cysteine as a Novel Polymethyl Methacrylate Resin Component: Protection against Cell Apoptosis and Genotoxicity. Oxidative Medicine and Cellular Longevity. doi: 10.1155/2019/1301736.
  4. Wang, Z. et al. (2019). Changes in the Proliferation Rate, Clonogenicity, and Radiosensitivity of Cultured Cells During and After Continuous Low-Dose-Rate Irradiation. Dose Response. 17(2):1559325819842733. doi: 10.1177/1559325819842733.
  5. Izumi, Y. et al. (2019). Suplatast tosilate reduces radiation-induced lung injury in mice through suppression of oxidative stress. Free Radic Biol Med. 136:52-59. doi: 10.1016/j.freeradbiomed.2019.03.024.
  6. Nakade, S. et al. (2018). Biased genome editing using the local accumulation of DSB repair molecules system. Nat Commun. 9(1):3270. doi: 10.1038/s41467-018-05773-6.
  7. Douiev, L. et al. (2018). The pathomechanism of cytochrome c oxidase deficiency includes nuclear DNA damage. Biochim Biophys Acta Bioenerg. 1859(9):893-900. doi: 10.1016/j.bbabio.2018.06.004.
  8. Douiev, L. et al. (2018). Cytochrome c oxidase deficiency, oxidative stress, possible antioxidant therapy and link to nuclear DNA damage. Eur J Hum Genet. 26(4):579-581. doi: 10.1038/s41431-017-0047-5.
  9. Shirasugi, M. et al. (2016). Normal human gingival fibroblasts undergo cytostasis and apoptosis after long-term exposure to butyric acid. Biochem. Biophys. Res. Commun. doi:10.1016/j.bbrc.2016.11.168.
  10. Wu, S. T. et al. (2016). Cellular effects induced by 17-β-estradiol to reduce the survival of renal cell carcinoma cells. J Biomed Sci. 23:67.
  11. Cheng, K. P. et al. (2016). Blue light modulates murine microglial gene expression in the absence of optogenetic protein expression. Sci Rep. doi:10.1038/srep21172.
  12. Ohashi, S. et al. (2014). Preclinical validation of talaporfin sodium-mediated photodynamic therapy for esophageal squamous cell carcinoma. PLoS One. 9:e103126.
  13. Dokic, I. et al. (2014). High resistance to X-rays and therapeutic carbon ions in glioblastoma cells bearing dysfunctional ATM associates with intrinsic chromosomal instability. Int J Radiat Biol. 91:157-165.
  14. Zhuge, C. C. et al. (2014). Fullerenol protects retinal pigment epithelial cells from oxidative stress–induced premature senescence via activating SIRT1. Invest Ophthalmol Vis Sci. 55:4628-4638.
  15. Matsuda, S. et al. (2014). An easy-to-use genotoxicity assay using EGFP-MDC1-expressing human cells. Gene Environ. 36:17-28.
  16. Edwards, A.K. et al. (2014).  A peptide inhibitor of synuclein-g reduces neovascularization of human endometriotic lesions.  Mol Hum Reprod. 10.1093/molehr/gau054.