Lentivirus-Associated p24 ELISA Kit

Lentivirus-Associated p24 ELISA Kit
  • More accurate titer measurements than traditional p24 assays
  • Proprietary technology removes non-viral free p24 protein from supernatant 
  • Lentivirus quantified on a standard microplate reader
  • HIV-1 p24 Standard included

 

Frequently Asked Questions about this product

General FAQs about Viral Gene Delivery

Video: Color Development in an ELISA

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QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA)
Catalog Number
VPK-107-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$2,975.00
QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA)
Catalog Number
VPK-107
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$690.00
QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated p24 ELiSA), Trial Size
Catalog Number
VPK-107-T
Size
32 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$355.00
Product Details

Measuring the HIV-1 p24 antigen is a long-established method for lentivirus quantitation. However, the traditional p24 ELISA detects both virus-associated p24 and free p24 generated by 293 cells during transient transfection. Free p24 can account for a substantial portion of total p24 in the supernatant. Therefore, the p24 ELISA typically overestimates the quantity of lentivirus present, leading to inaccurate titer measurements. Overestimation of titer leads to delivery of less virus than needed to your target cells, which can result in failed gene expression studies.

Our QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA) substantially minimizes this problem. A proprietary technology separates the lentivirus from non-viral free p24 in solution prior to running the ELISA portion of the assay. This allows you to more accurately determine the concentration of your lentivirus, which can lead to more successful gene expression studies.

Assay Principle for the QuickTiter Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA).

ViraBind™ Lentivirus Reagents Successfully Separate Free p24 from Lentivirus-Associated p24. Recombinant p24 diluted in culture medium was treated with ViraBind™ Lentivirus Reagents. The amount of p24 in the supernatant and pellet was measured according to the assay protocol.

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