Lentivirus-Associated p24 ELISA Kit

  • Proprietary technology separates virus-associated p24 from free p24
  • Minimizes overestimation of lentivirus titer common to traditional p24 ELISA kits
  • Lentivirus quantitation on a standard microplate reader
  • HIV-1 p24 Standard included

 

Frequently Asked Questions about this product

General FAQs about Viral Gene Delivery

Video: Color Development in an ELISA

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QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24)
Catalog Number
VPK-107-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$2,650.00
QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24)
Catalog Number
VPK-107
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$625.00
QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24), Trial Size
Catalog Number
VPK-107-T
Size
32 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$310.00
Product Details

Measuring the HIV-1 p24 antigen is a long-established method for lentivirus quantitation. However, the traditional p24 ELISA detects both virus-associated p24 and free p24 generated by 293 cells during transient transfection. Free p24 can account for a substantial portion of total p24 in the supernatant. Therefore, the ELISA typically overestimates the quantity of lentivirus present.

Our QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24) substantially minimizes this problem. A proprietary technology separates the lentivirus from free p24 in solution prior to running the ELISA portion of the assay.

Assay Principle for the QuickTiter Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA).

ViraBind™ Lentivirus Reagents Successfully Separate Free p24 from Lentivirus-Associated p24. Recombinant p24 diluted in culture medium was treated with ViraBind™ Lentivirus Reagents. The amount of p24 in the supernatant and pellet was measured according to the assay protocol.

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