Soft Agar Assays for Anchorage Independent Cell Growth

Neoplastic transformation occurs via a series of genetic and epigenetic alterations that yield a cell population that is capable of proliferating independently of both external and internal signals that normally restrain growth. Anchorage-independent growth is one of the hallmarks of cell transformation, which is considered the most accurate and stringent in vitro assay for detecting malignant transformation of cells.