Q: How is the gel released from the well?

A: After a two day culture, a sterile spatula is carefully inserted between the plate wall and the gel.  The spatula is then slowly moved around the wall in a circle to completely release the gel.   We use a small spatula with a thin, flat, edge and is the same spatula used for weighing out chemicals in milligram quantities. Prior to releasing the gel, the spatula should be sterilized with 70% ethanol.

Q: How do the Microfluidic Biochips work for studying cell adhesion?

Q: What is the assay principle for your ECM Cell Adhesion Assays?

A: Our Cell Adhesion Assays use an ECM coated plate for cell seeding.  The wells are then washed with PBS to remove any non-adherent cells.  The remaining adherent cells are stained and the dye is then extracted for quantification using a plate reader. 

Q: Can this be used with cells other than HUVECs?

A: This assay can be used with any endothelial cells.

Q: What is the composition of the ECM gel solution?