Q: Why am I getting high background?

Q: Can this kit be used on samples from any species?

A: Because the structure of 3-nitrotyrosine (3-NT) is the same regardless where the protein lysate sample is from, STA-305 is not species specific and can be used for any protein samples containing 3-NT.
 

Q: What samples types are suitable for this assay? 

A: Our AOPP Assay can be used for any samples containing AOPP above the kit detection limit, such as plasma, serum, tissue and cell lysates that have been stored at -80ºC for no more than one year.

Q: What is the sensitivity of this kit?

A: This kit can detect between 49 pg/mL and 200,000 pg/mL of 8-isoprostane, which is the range of the standard curve.

Q: Can I use your TBARS Assay to measure the TBARS level in my blood?

A: No, our TBARS Assay Kit is strictly for use by researchers in a laboratory. It cannot be used for diagnostic use to test patient samples.

Q: How long can I store my samples before testing for HNE adducts?

A: Free HNE is very unstable, but HNE adducts are very stable and samples can be stored at -80ºC for up to six months without HNE degradation.  Freeze-thaws should be avoided.

Q: Are EDTA plasma samples compatible with your kits?

Q: How does the sensitivity of your MDA ELISA Kit compare to the TBARS Assay?

Q: What part of the CML does the antibody bind to: the protein or CML itself? 

A: The monoclonal antibody used in our CML ELISA Kits recognizes the CML structure, rather than the protein.  

Q: Are EDTA plasma samples compatible with your kit?

A: EDTA will not interfere with our MG ELISA Kit and any anticoagulant can be used when preparing plasma samples.

Q: Is your methylglyoxal kit compatible with samples of any species?

Q: What structures are detected with your AGE Competitive ELISA Kit?