Q: How were these cell lines made?
A: Our stable cell lines had GFP, RFP, or luciferase introduced by either lentivirus transduction or plasmid transfection, followed by selection of stable clones.
Q: Can these cell lines be used in vivo?
Q: Does this antibody cross react with EGFP?
Q: What GFP variants will this antibody recognize?
Q: Will the proteins retrieved with this kit be in their native forms?
A: The proteins retrieved using this kit will be renatured and properly folded, since only correctly folded proteins are able to bind to the glutathione beads.
Q: What is the optimal detergent ratio?
Q: What species is this kit compatible with?
A: Our Rapid Antibody Purification Kit is a protein A column based purification kit that can be used to purify IgG from human, mouse, rabbit, pig, dog or cat.
Q: Can I use this kit with more than 5 mL of sample?
Q: How does the His-Tag Protein ELISA work?
Q: Will this kit detect EGFP?
Q: Will this kit detect EGFP?
A: This kit will detect fluorescence from EGFP. The recombinant GFP standard used in the kit is also EGFP.
Q: What is the protocol for lysing cells?
Q: Which detection method is more sensitive?
Cell proliferation is an increase in the number of cells resulting from the normal, healthy process by which cells grow and divide. In this regard, cell proliferation can be a good indicator of general cell health. Cells that are subject to a variety of disease states may exhibit different rates of proliferation than normal cells. Therefore, measuring rates of cell proliferation between different cell populations may provide insight into the relative health of those cells.