Q: Are the Small GTPase Assay Beads (Raf-RBD, PAK1-PBD and Rhotekin-RBD) species specific?

Q: I am concerned about being able to detect low levels of active small GTPase in my sample using your Small GTPase Activation Assays. Do you have any suggestions to ensure I get good results?

A: There are two things you can do to improve your results with our Small GTPase Activation Assays:

Q: What is the recommended lysis buffer?

Q: How were these cell lines made?

A: Our stable cell lines had GFP, RFP, or luciferase introduced by either lentivirus transduction or plasmid transfection, followed by selection of stable clones.

Q: Can these cell lines be used in vivo?

Q: Does this antibody cross react with EGFP?

Q: What GFP variants will this antibody recognize?

Q: Will the proteins retrieved with this kit be in their native forms?

A: The proteins retrieved using this kit will be renatured and properly folded, since only correctly folded proteins are able to bind to the glutathione beads.

Q: What is the optimal detergent ratio?

Q: What species is this kit compatible with? 

A: Our Rapid Antibody Purification Kit is a protein A column based purification kit that can be used to purify IgG from human, mouse, rabbit, pig, dog or cat. 

Q: Can I use this kit with more than 5 mL of sample?

Q: How does the His-Tag Protein ELISA work?

Q: Will this kit detect EGFP?