Q: I am concerned about being able to detect low levels of active small GTPase in my sample using your Small GTPase Activation Assays. Do you have any suggestions to ensure I get good results?
A: There are two things you can do to improve your results with our Small GTPase Activation Assays:
Q: What is the recommended lysis buffer?
Q: How were these cell lines made?
A: Our stable cell lines had GFP, RFP, or luciferase introduced by either lentivirus transduction or plasmid transfection, followed by selection of stable clones.
Q: Can these cell lines be used in vivo?
Q: Does this antibody cross react with EGFP?
Q: What GFP variants will this antibody recognize?
Q: Will the proteins retrieved with this kit be in their native forms?
A: The proteins retrieved using this kit will be renatured and properly folded, since only correctly folded proteins are able to bind to the glutathione beads.
Q: What is the optimal detergent ratio?
Q: What species is this kit compatible with?
A: Our Rapid Antibody Purification Kit is a protein A column based purification kit that can be used to purify IgG from human, mouse, rabbit, pig, dog or cat.
Q: Can I use this kit with more than 5 mL of sample?
Q: How does the His-Tag Protein ELISA work?
Q: Will this kit detect EGFP?
Q: Will this kit detect EGFP?
A: This kit will detect fluorescence from EGFP. The recombinant GFP standard used in the kit is also EGFP.
Q: What is the protocol for lysing cells?