FAQ: Lentiviral Expression and Packaging Systems
Q: What is required to make lentivirus?
A: To make lentivirus, you need:
Q: What is required to make lentivirus?
A: To make lentivirus, you need:
Q: How are your 293LTV cells different from HEK293T cells?
A: The 293LTV cell line is a permanent cell line derived from HEK293T and was selected to have a high lentiviral yield. This cell line is fast growing and has a firm attachment to the culture plate, which can sometimes be problematic with standard 293T cells.
Q: Is there a positive control cell line that is easily infected by lentivirus?
Q: What cells can be infected with adenovirus?
Q: What is the RAPAd® system?
Q: How much adenovirus should be used for transduction?
Q: Which serotypes are your premade adenoviruses?
A: All of our Premade Recombinant Adenoviruses contain the Ad5 backbone with E1 and E3 deletions.
Q: Your ViraBind™ Adenovirus Purification Kit is listed as a 10 prep kit. Why does the kit only contain 5 filters?
Q: What are the main differences between your two Adenovirus Purification Kits?
A: There are a couple of differences:
1. #VPK-099 uses a spin column while #VPK-5112 uses a purification resin.
Q: How are these cells improved over normal 293 cells?