Q: How does the Radius™ assay work?
A: Our Radius assay uses a plate that contains a biocompatible hydrogel at the center of the well; seeded cells will attach everywhere but on the hydrogel. After cells attach, the gel is removed by addition of a removal solution and the assay begins.
Q: What endothelial cells are compatible with this assay?
A: We use HUVEC cells, but any endothelial cells can be used.
Q: How can I confirm that the insert is evenly covered with endothelial cells?
Q: What is the difference between your product #CBA-101-COL where collagen is pre-coated on the underside (bottom) of the membrane, and your product #CBA 111-COL where collagen is coated on top of the membrane?
Q: What is the detection limit with the CyQuant® dye?
Q: Can the inserts be reused?
A: Our 24-well migration assays contain 12 individual, removable inserts made out of polycarbonate. Any unused inserts can be stored at 4ºC until needed, however the inserts should not be used more than once.
Q: Can the inserts be stored after staining migratory cells?
Q: How is chemotaxis measured using a Boyden Chamber system?
Q: Does the entire 96-well Invasion Chamber plate need to be used in one experiment?
Q: Is the colorimetric or fluorometric kit more sensitive?
Q: I have never run an assay using a Boyden Chamber. Would I have a better chance of success with your Cell Invasion kits if I use the 24-well or 96-well format?
Q: What size should the tumors be prior to mincing?
A: The size of the tumor used in the assay is not critical because there is a step that adjusts for cell number (step 14 of the Assay Protocol found in the product manual). It is difficult to recommend a size because the cell density will vary, but we used a 160mg lung tumor when developing this assay. If the tumor is large enough to be minced, it will likely have enough cells for the assay.