Radius™ 24-Well Cell Migration Assay

Radius™ 24-Well Cell Migration Assay
  • Proprietary biocompatible hydrogel creates a circular area across which cells may migrate following gel removal
  • Versatile plate format allows use with cells of any size; no need to worry about selecting cell culture inserts with the proper pore size
  • Allows qualitative, quantitative, endpoint or real-time analysis
  • Adaptable to liquid handling equipment and HCS instrumentation

Software Analysis Tools for use with this product

Frequently Asked Questions about this product

Email To BuyerPrint this PageCopy Link
Ordering

Please contact your distributor for pricing.

Radius™ 24-Well Cell Migration Assay
Catalog Number
CBA-125
Size
24 assays
Detection
Microscopy
Manual/Data Sheet Download
SDS Download
Price
$395.00
Radius™ 24-Well Cell Migration Assay
Catalog Number
CBA-125-5
Size
5 x 24 assays
Detection
Microscopy
Manual/Data Sheet Download
SDS Download
Price
$1,695.00
Product Details

The Radius™ Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius™ assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius™ gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius™ gel, creating a cell-free zone. Following cell seeding the Radius™ gel is removed, allowing migratory cells to move across the area and close the gap.

Assay Principle.

Various Detection Methods with Radius™ Cell Migration Assay. HeLa cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were stained according to the assay protocol with Cell Stain Solution, Calcein AM (not included in kit), or DAPI.

Cell Migration Time Course. HeLa, HT1080 and NIH3T3 cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were allowed to migrate for the various times shown.

Inhibition of HeLa Cell Migration by Cytochalasin D. HeLa cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were allowed to migrate for 24 hours in the presence of various concentrations of Cytochalasin D.

Recent Product Citations
  1. Gimeno-Valiente, F. et al. (2020). EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness. Sci Rep. 10(1):3723. doi: 10.1038/s41598-020-60476-7.
  2. Malinee, M. et al. (2019). Targeted suppression of metastasis regulatory transcription factor SOX2 in various cancer cell lines using a sequence-specific designer pyrrole–imidazole polyamide. Bioorganic & Medicinal Chemistry. doi: 10.1016/j.bmc.2019.115248.
  3. Mathews Samuel, S. et al. (2019). Treatment with a Combination of Metformin and 2-Deoxyglucose Upregulates Thrombospondin-1 in Microvascular Endothelial Cells: Implications in Anti-Angiogenic Cancer Therapy. Cancers (Basel). 11(11). pii: E1737. doi: 10.3390/cancers11111737.
  4. Gimeno-Valiente, F. et al. (2019). ZNF518B gene up-regulation promotes dissemination of tumour cells and is governed by epigenetic mechanisms in colorectal cancer. Sci Rep. 9(1):9339. doi: 10.1038/s41598-019-45411-9.
  5. Yeo, M.S. et al. (2019). FBXW5 Promotes Tumorigenesis and Metastasis in Gastric Cancer via Activation of the FAK-Src Signaling Pathway. Cancers (Basel). 11(6). pii: E836. doi: 10.3390/cancers11060836.
  6. Lu. C.C. et al. (2019). Biological analysis of flexor tendon repair-failure stump tissue. Bone Joint Res. 8:232-245. doi: 10.1302/2046-3758.86.BJR2018-0239.R1.
  7. Ghoshal, A. et al. (2019). Extracellular vesicle-dependent effect of RNA-binding protein IGF2BP1 on melanoma metastasis. Oncogene. doi: 10.1038/s41388-019-0797-3.
  8. Wilkinson, A.W. et al. (2019). SETD3 is an actin histidine methyltransferase that prevents primary dystocia. Nature. 565(7739):372-376. doi: 10.1038/s41586-018-0821-8.
  9. Reckelhoff, C.R. et al. (2019). In vitro effects of the chemotherapy agent water-soluble micellar paclitaxel (Paccal Vet) on canine hemangiosarcoma cell lines. Vet Comp Oncol. 17(1):32-41. doi: 10.1111/vco.12442.
  10. Aydemir, E. et al. (2018). The effect of TWIST silencing in metastatic chordoma cells. Turk J Biol. 42: 279-285. doi: 10.3906/biy-1801-17.
  11. Tanikawa, N. et al. (2017). Interferon Tau Regulates Cytokine Production and Cellular Function in Human Trophoblast Cell Line. J Interferon Cytokine Res. 37(10):456-466. doi: 10.1089/jir.2017.0057.
  12. Kuroda, T. et al. (2017). Identification of a Gene Encoding Slow Skeletal Muscle Troponin T as a Novel Marker for Immortalization of Retinal Pigment Epithelial Cells. Sci Rep. 7(1):8163. doi: 10.1038/s41598-017-08014-w.
  13. Lang, L. et al. (2017). Combined targeting of Arf1 and Ras potentiates anticancer activity for prostate cancer therapeutics. J Exp Clin Cancer Res. 36(1):112. doi: 10.1186/s13046-017-0583-4.
  14. Arisi, M.F. et al. (2017). Myocardial apoptosis and mesenchymal stem cells with acute exercise. Physiol. Rep. doi:10.14814/phy2.13297.
  15. Johansen, C. et al. (2017). STAT2 is involved in the pathogenesis of psoriasis by promoting CXCL11 and CCL5 production by keratinocytes. PLoS One. 12(5):e0176994.
  16. Feng, X. et al. (2017). Connective tissue growth factor is not necessary for haze formation in excimer laser wounded mouse corneas. PLoS One. doi: 10.1371/journal.pone.0172304.
  17. Lee, Y-Y. et al. (2016). Dynamin 2 inhibitors as novel therapeutic agents against cervical cancer cells. Anticancer Res. 36:6381-6388.
  18. Kubo, E. et al. (2016). FGF2 antagonizes aberrant TGFß regulation of tropomyosin: role for posterior capsule opacity. J. Cell. Mol. Med. doi:10.1111/jcmm.13030.
  19. Arai, Y. et al. (2016). Enhancement of matrix metalloproteinase-2 (MMP-2) as a potential chondrogenic marker during chondrogenic differentiation of human adipose-derived stem cells. Int J Mol Sci. doi:10.3390/ijms17060963.
  20. Barnes, J. W. et al. (2016). BMPR2 mutation-independent mechanisms of disrupted BMP signaling in IPAH. Am J Respir Cell Mol Biol. doi:10.1165/rcmb.2015-0402OC.
  21. Mohamedi, Y. et al. (2016). Fibulin-5 downregulates Ki-67 and inhibits proliferation and invasion of breast cancer cells. Int J Oncol. 48:1447-1456.
  22. Yu, S. H. et al. (2016). Anti-proliferative effects of rutin on OLETF rat vascular smooth muscle cells stimulated by glucose variability. Yonsei Med J. 57:373-381.
  23. Sanna, V. et al. (2015). Nanoencapsulation of natural triterpenoid celastrol for prostate cancer treatment. Int J Nanomedicine. 10:6835-46.
  24. Povero, D. et al. (2015). Lipid-induced hepatocyte-derived extracellular vesicles regulate hepatic stellate cell via microRNAs targeting PPAR-γ. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2015.07.007.
  25. Douchi, D. et al. (2015). Silencing of leucine-rich repeat flightless-1-interacting protein 1 reverses the epithelial-mesenchymal transition via inhibition of the Wnt/β-catenin signaling pathway. Cancer Lett. doi:10.1016/j.canlet.2015.05.023.
  26. Nishikawa, M. et al. (2015).  Enhanced sensitivity to sunitinib by inhibition of Akt1 expression in human castration-resistant prostate cancer PC3 cells both in vitro and in vivo. Urology.  85:1215-e1.
  27. Pu, J. et al. (2015). BORC, a multisubunit complex that regulates lysosome positioning. Dev Cell.  33:176-188.
  28. Ge, C. et al. (2015). Role of Runx2 phosphorylation in prostate cancer and association with metastatic disease. Oncogene. doi:10.1038/onc.2015.91.
  29. Tang, Y. et al. (2015). Effect of taurine on prostate-specific antigen level and migration in human prostate cancer cells. Adv Exp Med Biol.  doi: 10.1007/978-3-319-15126-7_18.
  30. Choi, E. J. et al. (2015). Effect of taurine on in vitro migration of MCF-7 and MDA-MB-231 human breast carcinoma cells. Adv Exp Med Biol.  doi: 10.1007/978-3-319-15126-7_17.