Radius™ 24-Well Cell Migration Assay

  • Proprietary biocompatible hydrogel creates a circular area across which cells may migrate following gel removal
  • Versatile plate format allows use with cells of any size; no need to worry about selecting cell culture inserts with the proper pore size
  • Allows qualitative, quantitative, endpoint or real-time analysis
  • Adaptable to liquid handling equipment and HCS instrumentation

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Radius™ 24-Well Cell Migration Assay
Catalog Number
24 assays
Manual/Data Sheet Download
SDS Download
Radius™ 24-Well Cell Migration Assay
Catalog Number
5 x 24 assays
Manual/Data Sheet Download
SDS Download
Product Details

The Radius™ Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius™ assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius™ gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius™ gel, creating a cell-free zone. Following cell seeding the Radius™ gel is removed, allowing migratory cells to move across the area and close the gap.

Assay Principle.

Various Detection Methods with Radius™ Cell Migration Assay. HeLa cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were stained according to the assay protocol with Cell Stain Solution, Calcein AM (not included in kit), or DAPI.

Cell Migration Time Course. HeLa, HT1080 and NIH3T3 cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were allowed to migrate for the various times shown.

Inhibition of HeLa Cell Migration by Cytochalasin D. HeLa cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were allowed to migrate for 24 hours in the presence of various concentrations of Cytochalasin D.

Recent Product Citations
  1. Lee, Y-Y. et al. (2016). Dynamin 2 inhibitors as novel therapeutic agents against cervical cancer cells. Anticancer Res. 36:6381-6388.
  2. Kubo, E. et al. (2016). FGF2 antagonizes aberrant TGFß regulation of tropomyosin: role for posterior capsule opacity. J. Cell. Mol. Med. doi:10.1111/jcmm.13030.
  3. Arai, Y. et al. (2016). Enhancement of matrix metalloproteinase-2 (MMP-2) as a potential chondrogenic marker during chondrogenic differentiation of human adipose-derived stem cells. Int J Mol Sci. doi:10.3390/ijms17060963.
  4. Barnes, J. W. et al. (2016). BMPR2 mutation-independent mechanisms of disrupted BMP signaling in IPAH. Am J Respir Cell Mol Biol. doi:10.1165/rcmb.2015-0402OC.
  5. Mohamedi, Y. et al. (2016). Fibulin-5 downregulates Ki-67 and inhibits proliferation and invasion of breast cancer cells. Int J Oncol. 48:1447-1456.
  6. Yu, S. H. et al. (2016). Anti-proliferative effects of rutin on OLETF rat vascular smooth muscle cells stimulated by glucose variability. Yonsei Med J. 57:373-381.
  7. Sanna, V. et al. (2015). Nanoencapsulation of natural triterpenoid celastrol for prostate cancer treatment. Int J Nanomedicine. 10:6835-46.
  8. Povero, D. et al. (2015). Lipid-induced hepatocyte-derived extracellular vesicles regulate hepatic stellate cell via microRNAs targeting PPAR-γ. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2015.07.007.
  9. Douchi, D. et al. (2015). Silencing of leucine-rich repeat flightless-1-interacting protein 1 reverses the epithelial-mesenchymal transition via inhibition of the Wnt/β-catenin signaling pathway. Cancer Lett. doi:10.1016/j.canlet.2015.05.023.
  10. Nishikawa, M. et al. (2015).  Enhanced sensitivity to sunitinib by inhibition of Akt1 expression in human castration-resistant prostate cancer PC3 cells both in vitro and in vivo. Urology.  85:1215-e1.
  11. Pu, J. et al. (2015). BORC, a multisubunit complex that regulates lysosome positioning. Dev Cell.  33:176-188.
  12. Ge, C. et al. (2015). Role of Runx2 phosphorylation in prostate cancer and association with metastatic disease. Oncogene. doi:10.1038/onc.2015.91.
  13. Tang, Y. et al. (2015). Effect of taurine on prostate-specific antigen level and migration in human prostate cancer cells. Adv Exp Med Biol.  doi: 10.1007/978-3-319-15126-7_18.
  14. Choi, E. J. et al. (2015). Effect of taurine on in vitro migration of MCF-7 and MDA-MB-231 human breast carcinoma cells. Adv Exp Med Biol.  doi: 10.1007/978-3-319-15126-7_17.
  15. Kim, E. K. et al. (2015). First evidence that ecklonia cava-derived dieckol attenuates MCF-7 human breast carcinoma cell migration. Mar Drugs. 13:1785-1797.
  16. Langfelder, A. et al. (2015). Extracellular acidosis impairs P2Y receptor-mediated Ca2+ signalling and migration of microgliaCell Calcium. doi: 10.1016/j.ceca.2015.01.004.
  17. Fontanil, T. et al. (2014). Interaction between the ADAMTS-12 metalloprotease and fibulin-2 induces tumor-suppressive effects in breast cancer cells. Oncotarget. 5:1253-1264.
  18. Liang, D. et al. (2014). miRNA-940 reduction contributes to human Tetralogy of Fallot development. J Cell Mol Med. 18:1830-1839.
  19. Wang, Y. et al. (2014). Dystrophin is a tumor suppressor in human cancers with myogenic programs. Nature genetics. 46:601-606.
  20. Tanoury, Z, et al. (2014). Genes involved in cell adhesion and signaling: a new repertoire of retinoic acid receptor target genes in mouse embryonic fibroblasts. J. Cell Sci. 127:521-533.
  21. Kasem, K. et al. (2014). JK1 (FAM134B) represses cell migration in colon cancer: a functional study of a novel gene. Exp Mol Pathol. 97:99-104.
  22. Li, Z. et al. (2014). Mammalian diaphanous-related formin 1 is required for motility and invadopodia formation in human U87 glioblastoma cells. Int J Mol Med. 33:383-391.
  23. Cersosimo, E. et al. (2014) Acute insulin resistance stimulates and insulin sensitization attenuates vascular smooth muscle cell migration and proliferation. Physiol Rep. 2:e12123.
  24. Qiang, L. et al. (2014).  Regulation of cell proliferation and migration by p62 through stabilization of Twist1. Proc Natl Acad Sci U S A. 111:9241-9246.
  25. Sriram, S. et al.(2013). Triple Combination of siRNAs Targeting TGFβ1, TGFβR2, and CTGF Enhances Reduction of Collagen I and Smooth Muscle Actin in Corneal Fibroblasts. Invest. Opthalmol. Vis. Sci. 54:8214-8223 (#CBA-125).
  26. Robinson, P. et al. (2013). MicroRNA Signature in Wound Healing Following Excimer Laser Ablation: Role of miR-133b on TGFβ1, CTGF, SMA, and COL1A1 Expression Levels in Rabbit Corneal Fibroblasts. J. Cell Sci. 126:4769-4781 (#CBA-125).
  27. Sun, J. et al. (2013). Targeting the Metastasis Suppressor, NDRG1, Using Novel Iron Chelators: Regulation of Stress Fiber-Mediated Tumor Cell Migration via Modulation of the ROCK1/pMLC2 Signaling Pathway. Mol. Pharmacol. 83:454-469 (#CBA-125).
  28. Wong, B. et al. (2013). Adrenomedullin Enhances Invasion of Human Extravillous Cytotrophoblast-Derived Cell Lines by Regulation of Urokinase Plasminogen Activator Expression and S-Nitrosylation. Biol Reprod. 88:34 (#CBA-126).
  29. Ichikawa, A. et al. (2013). CXCL10-CXCR3 Enhances the Development of Neutrophil-mediated Fulminant Lung Injury of Viral and Nonviral Origin. Am. J. Respir. Crit. Care. Med. 187:65-77 (#CBA-126).
  30. Apostolos, K. et al. (2013). Increased Susceptibility of Melanin-Concentrating Hormone-Deficient Mice to Infection with Salmonella enterica Serovar Typhimurium. Infect. Immun. 81: 166-172 (#CBA-125).
  31. Smith, K. et al. (2012). Human Family with Sequence Similarity 60 Member A (FAM60A) Protein: a New Subunit of the Sin3 Deacetylase Complex. Mol. Cell. Proteomics. 11:1815-1828 (#CBA-125).
  32. Young, S. et al. (2012). Rapid Protein Kinase D1 Signaling Promotes Migration of Intestinal Epithelial Cells. Am J Physiol Gastrointest Liver Physiol. 303: G356-G366 (#CBA-125).
  33. Larive, R.M. et al. (2012). The Ras-like Protein R-Ras2/TC21 is Important for Proper Mammary Gland Development. Mol. Biol. Cell. 23:2373-2387(#CBA-125).
  34. Coulouarn, C. et al. (2012). Hepatocyte–Stellate Cell Cross-Talk in the Liver Engenders a Permissive Inflammatory Microenvironment That Drives Progression in Hepatocellular Carcinoma. Cancer Res. 72: 2533-2542 (#CBA-126). 
  35. Chuang, T.D. et al. (2012). miR-93/106b and Their Host Gene, MCM7, Are Differentially Expressed in Leiomyomas and Functionally Target F3 and IL-8. Mol. Endocrin. 26: 1028-1042 (#CBA-125).
  36. Alcolea, S. et al. (2012).Interaction Between Head and Neck Squamous Cell Carcinoma Cells and Fibroblasts in the Biosynthesis of PGE2. J.Lipid Res. 53:630-642(#CBA-126).