Radius™ 24-Well Cell Migration Assay, Collagen I Coated

Proprietary biocompatible hydrogel creates a circular area across which cells may migrate following gel removal
Versatile plate format allows use with cells of any size; no need to worry about selecting cell culture inserts with the proper pore size
Allows qualitative, quantitative, endpoint or real-time analysis
24-well plates pre-coated with Collagen I to help promote cell adhesion
Adaptable to liquid handling equipment and HCS instrumentation

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Radius™ 24-Well Cell Migration Assay (Collagen I Coated)

Catalog Number	CBA-125-COL
Size	24 assays
Detection	Microscopy
Manual/Data Sheet	Download
SDS	Download
Price	$550.00

Product Details

The Radius™ Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius™ assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius™ gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius™ gel, creating a cell-free zone. Following cell seeding the Radius™ gel is removed, allowing migratory cells to move across the area and close the gap.

Assay Principle.

Various Detection Methods with Radius™ Cell Migration Assay. HeLa cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were stained according to the assay protocol with Cell Stain Solution, Calcein AM (not included in kit), or DAPI.

Cell Migration Time Course. HeLa, HT1080 and NIH3T3 cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were allowed to migrate for the various times shown.

Inhibition of HeLa Cell Migration by Cytochalasin D. HeLa cells were seeded at 100,000 cells/well overnight. After removal of Radius™ Gel, cells were allowed to migrate for 24 hours in the presence of various concentrations of Cytochalasin D.