Intracellular ROS Assay

  • Quick ~1 hour protocol
  • Highly sensitive to 10 pM
  • Detects the presence of various ROS species


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General FAQs about Oxidative Stress

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OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence)
Catalog Number
96 Assays
Manual/Data Sheet Download
SDS Download
OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence)
Catalog Number
5 x 96 Assays
Manual/Data Sheet Download
SDS Download
OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence), Trial Size
Catalog Number
20 assays
Manual/Data Sheet Download
SDS Download
Product Details

The OxiSelect™ ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

Assay Principle.

ROS in HeLa Cells Treated with Hydrogen Peroxide. 50,000 HeLa cells in a 96-well plate were pretreated with 1 mM DCFH-DA for 60 minutes at 37ºC. Cells were then treated with hydrogen peroxide for 20 minutes.

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