Rho Kinase (ROCK) Activity Immunoblot Kit

Rho Kinase (ROCK) Activity Immunoblot Kit
  • Safe non-radioactive Western blot assay format
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ROCK Activity Immunoblot Kit
Catalog Number
20 blots
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Product Details

Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20).

Our ROCK Activity Immunoblot Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates.

Recent Product Citations
  1. Kim, K.B. et al. (2022). WNT5A-RHOA signaling is a driver of tumorigenesis and represents a therapeutically actionable vulnerability in small cell lung cancer. Cancer Res. doi: 10.1158/0008-5472.CAN-22-1170.
  2. Onodera, K. et al. (2018). Growth inhibition of KRAS‑ and EGFR‑mutant lung adenocarcinoma by cosuppression of STAT3 and the SRC/ARHGAP35 axis. Oncol Rep. 40(3):1761-1768. doi: 10.3892/or.2018.6536.
  3. Rothschild, P.R. et al. (2017). ROCK-1 mediates diabetes-induced retinal pigment epithelial and endothelial cell blebbing: Contribution to diabetic retinopathy. Sci Rep. 7(1):8834. doi: 10.1038/s41598-017-07329-y.
  4. Watts, B. A. et al. (2016). High mobility group box 1 inhibits HCO3-absorption in medullary thick ascending limb through RAGE-Rho-ROCK-mediated inhibition of basolateral Na+/H+ exchange. Am J Physiol Renal Physiol. doi:10.1152/ajprenal.00185.2016.
  5. Seo, J. H. et al. (2016). Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes. RSC Adv. doi:10.1039/C6RA03967G.
  6. Tocci, P. et al. (2016). Endothelin-1/endothelin A receptor axis activates RhoA GTPase in epithelial ovarian cancer. Life Sci.doi:10.1016/j.lfs.2016.01.008.
  7. Su, C. C. et al. (2015). Phenotypes of trypsin-and collagenase-prepared bovine corneal endothelial cells in the presence of a selective Rho kinase inhibitor, Y-27632. Mol Vis. 21:633-643. 
  8. Gross, P. et al. (2015). Para-cresyl sulfate acutely impairs vascular reactivity and induces vascular remodeling. J Cell Physiol.  doi: 10.1002/jcp.25018.
  9. Sailland, J. et al. (2014). Estrogen-Related Receptor α Decreases RHOA Stability to Induce Orientated Cell Migration. Proc Natl Acad Sci U S A. 111:15108-15113.
  10. Georgess, D. et al. (2014). Comparative Transcriptomics Reveals RhoE as a Novel Regulator of Actin Dynamics in Bone-Resorbing Osteoclasts. Mol. Biol. 25:380-396.
  11. Chandrasekharan, U. et al. (2013). Release of Nonmuscle Myosin II from the Cytosolic Domain of Tumor Necrosis Factor Receptor 2 Is Required for Target Gene Expression. Sci. Signal. 6: ra60.
  12. Gupta, N. et al. (2012). Soluble Epoxide Hydrolase: Sex Differences and Role in Endothelial Cell Survival. Arterioscler Throm Vasc Biol. 32:1936-1942.
  13. Forero, D.G. et al.(2012).Endogenous Rho-Kinase Signaling Maintains Synaptic Strength by Stabilizing the Size of the Readily Releasable Pool of Synaptic Vesicles. J.Biol Chem. 287:1600-1608.
  14. Wang, J.N. et al. (2011). Response Gene to Complement 32 Promotes Vascular Lesion Formation Through Stimulation of Smooth Muscle Cell Proliferation and Migration. Arterioscler Thromb Vasc Biol. 31:e19-e26.
  15. Li, Z. et al. (2009). TrkBT1 Induces Liver Metastasis of Pancreatic Cancer Cells by Sequestering Rho GDP Dissociation Inhibitor and Promoting RhoA Activation. Cancer Res. 69:7851-7859.
  16. Xiao, L. et al. (2009). ROCK Mediates Phorbol Ester-Induced Apoptosis in Prostate Cancer Cells Via p21-Cip1 Upr-Egulation and JNK. J. Biol. Chem. 284:29365-29375.