Total Cholesterol Assay Kits

Total Cholesterol Assay Kits
  • Suitable for use with serum, plasma, lysate, or tissue samples
  • Cholesterol standard included
  • Available with colorimetric or fluorometric detection


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Total Cholesterol Assay Kit (Fluorometric)
Catalog Number
192 assays
Manual/Data Sheet Download
SDS Download
Total Cholesterol Assay Kit (Colorimetric)
Catalog Number
192 assays
Manual/Data Sheet Download
SDS Download
Product Details

Cell Biolabs’ Total Cholesterol Assay Kits measure the total cholesterol within serum, plasma, cell lysate, or tissue samples. The assays will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme.

Assay Principle for the Total Cholesterol Assay (Fluorometric).

Cholesterol Standard Curve.

Recent Product Citations
  1. Pasello, M. et al. (2022). ABCA6 affects the malignancy of Ewing sarcoma cells via cholesterol-guided inhibition of the IGF1R/AKT/MDM2 axis. Cell Oncol (Dordr). doi: 10.1007/s13402-022-00713-5 (#STA-384).
  2. Shang, Y. et al. (2022). A CHCHD6-APP axis connects amyloid and mitochondrial pathology in Alzheimer's disease. Acta Neuropathol. doi: 10.1007/s00401-022-02499-0 (#STA-384).
  3. Hwang, S.M. et al. (2022). Preventive and Therapeutic Effects of Krill Oil on Obesity and Obesity-Induced Metabolic Syndromes in High-Fat Diet-Fed Mice. Mar Drugs. 20(8):483. doi: 10.3390/md20080483 (#STA-384).
  4. Cho, I.J. et al. (2022). Lemon Balm and Corn Silk Mixture Alleviates Metabolic Disorders Caused by a High-Fat Diet. Antioxidants (Basel). 11(4):730. doi: 10.3390/antiox11040730 (#STA-384).
  5. Choi, J.Y. et al. (2022). Combination Effects of Metformin and a Mixture of Lemon Balm and Dandelion on High-Fat Diet-Induced Metabolic Alterations in Mice. Antioxidants (Basel). 11(3):580. doi: 10.3390/antiox11030580 (#STA-384).
  6. Zhao, Y. et al. (2022). ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models. Nat Commun. 13(1):1121. doi: 10.1038/s41467-022-28769-9 (#STA-384).
  7. Alsabaani, N.A. et al. (2022). Maslinic Acid Protects against Streptozotocin-Induced Diabetic Retinopathy by Activating Nrf2 and Suppressing NF-κB. J Ophthalmol. 2022:3044202. doi: 10.1155/2022/3044202 (#STA-384).
  8. Chen, T.Y. et al. (2022). Effects of the Water Extract of Fermented Rice Bran on Liver Damage and Intestinal Injury in Aged Rats with High-Fat Diet Feeding. Plants (Basel). 11(5):607. doi: 10.3390/plants11050607 (#STA-384).
  9. Valdivia, A.O. & Bhattacharya, S.K. (2022). Lyso-Lipid-Induced Oligodendrocyte Maturation Underlies Restoration of Optic Nerve Function. eNeuro. 9(1):ENEURO.0429-21.2022. doi: 10.1523/ENEURO.0429-21.2022 (#STA-384).
  10. Landowski, M. et al. (2022). A mutation in transmembrane protein 135 impairs lipid metabolism in mouse eyecups. Sci Rep. 12(1):756. doi: 10.1038/s41598-021-04644-3 (#STA-384).
  11. Wang, Y. et al. (2021). N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism. Nat Commun. 12(1):6314. doi: 10.1038/s41467-021-26718-6 (#STA-390).
  12. Lee, H. et al. (2021). Soybean-Derived Peptides Attenuate Hyperlipidemia by Regulating Trans-Intestinal Cholesterol Excretion and Bile Acid Synthesis. Nutrients. 14(1):95. doi: 10.3390/nu14010095 (#STA-384).
  13. Bhat, N. et al. (2021). Dyrk1b promotes hepatic lipogenesis by bypassing canonical insulin signaling and directly activating mTORC2 in mice. J Clin Invest. doi: 10.1172/JCI153724 (#STA-384).
  14. McGruer, V. et al. (2021). Exposure to Deepwater Horizon crude oil increases free cholesterol in larval red drum (Sciaenops ocellatus). Aquat Toxicol. 241:105988. doi: 10.1016/j.aquatox.2021.105988 (#STA-384).
  15. Abdelwahed, K.S. et al. (2021). PCSK9 Axis-Targeting Pseurotin A as a Novel Prostate Cancer Recurrence Suppressor Lead. ACS Pharmacol. Transl. Sci. doi: 10.1021/acsptsci.1c00145 (#STA-384).
  16. Devarshi, P.P. et al. (2021). A single bout of cycling exercise induces nucleosome repositioning in the skeletal muscle of lean and overweight/obese individuals. Diabetes Obes Metab. doi: 10.1111/dom.14541 (#STA-384).
  17. Elsyade, R. et al. (2021). Hazards of Chronic Exposure to Nonylphenol: Concomitant Effect on Non-alcoholic Fatty Liver Disease in Male Albino Rats. Open Access Maced J Med Sci. 9(A):548-555 doi: 10.3889/oamjms.2021.6237 (#STA-384).
  18. Rekhi, U.R. et al. (2021).  Impact of a CD36 Inhibitor on Porphyromonas gingivalis Mediated Atherosclerosis. Arch Oral Biol. doi: 10.1016/j.archoralbio.2021.105129 (#STA-384).
  19. Basile, A.J. et al. (2021). A four-week urban diet impairs vasodilation but not nutritional physiology in wild-caught mourning doves (Zenaida macroura). Physiol Biochem Zool. doi: 10.1086/714831 (#STA-384).
  20. Kohlhaas, J. et al. (2021). Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression. Exp Cell Res. doi: 10.1016/j.yexcr.2020.112446 (#STA-384).
  21. Ganguly, S. et al. (2021). Non-alcoholic Steatohepatitis and HCC in a Hyperphagic Mouse Accelerated by Western Diet. Cell Mol Gastroenterol Hepatol. doi: 10.1016/j.jcmgh.2021.05.010 (#STA-384).
  22. Kumar, S. et al. (2021). Cocaine-Specific Effects on Exosome Biogenesis in Microglial Cells. Neurochem Res. doi: 10.1007/s11064-021-03231-2 (#STA-384).
  23. Lee, H.B. et al. (2020). Molokhia leaf extract prevents gut inflammation and obesity. J Ethnopharmacol. doi: 10.1016/j.jep.2020.112866 (#STA-384).
  24. Mou, Y. et al. (2020). Impaired lipid metabolism in astrocytes underlies degeneration of cortical projection neurons in hereditary spastic paraplegia. Acta Neuropathol Commun. 8(1):214. doi: 10.1186/s40478-020-01088-0 (#STA-384).
  25. Deng, Q. et al. (2020). Dietary Lactic Acid Bacteria Modulate Yolk Components and Cholesterol Metabolism by Hmgr Pathway in Laying Hens. Braz. J. Poult. 22(3):eRBCA-2020-1261. doi: 10.1590/1806-9061-2020-1261 (#STA-390).
  26. Singhal, A. et al. (2020). 2-Hydroxypropyl-gamma-cyclodextrin overcomes NPC1 deficiency by enhancing lysosome-ER association and autophagy. Sci Rep. 10(1):8663. doi: 10.1038/s41598-020-65627-4 (#STA-390).
  27. Kidnapillai, S. et al. (2019). Drugs Used in the Treatment of Bipolar Disorder and their Effects on Cholesterol Biosynthesis- A Possible Therapeutic Mechanism. World J Biol Psychiatry. doi: 10.1080/15622975.2019.1669823 (#STA-390).
  28. Le Menn, G. et al. (2019). Decrease in αβ/γδ T-cell ratio is accompanied by a reduction in high-fat diet-induced weight gain, insulin resistance, and inflammation. FASEB J. 33(2):2553-2562. doi: 10.1096/fj.201800696RR (#STA-390).
  29. Martiskainen H, et al. (2017). DHCR24 exerts neuroprotection upon inflammation-induced neuronal death. J Neuroinflammation. 14(1):215. doi: 10.1186/s12974-017-0991-6 (#STA-390).
  30. Hsieh, H.Y. et al. (2017). Quantification of Endogenous Cholesterol in Human Serum on Paper Using Direct Analysis in Real Time Mass Spectrometry. Anal Chem. 89(11):6146-6152. doi: 10.1021/acs.analchem.7b00943 (#STA-390).