Total Phosphatidic Acid Assay Kit

Total Phosphatidic Acid Assay Kit
  • Detects total phosphatidic acid content (PA and LPA) in cell lysate samples
  • Detection sensitivity of approximately 5 µM phosphatidic acid
  • Phosphatidic Acid standard curve included
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Total Phosphatidic Acid Assay Kit
Catalog Number
MET-5019
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$415.00
Product Details

Phosphatidic Acid (PA) is a phospholipid that serves three main roles in the cell: it is a precursor for lipid biosynthesis, provides membrane structure, and acts as a signaling lipid by recruiting cytosolic proteins to the cell membrane. PA concentrations are maintained at low levels in the cell by phospholipid phosphatases, which convert PA to diacylglycerol (DAG). Measurement of cellular PA levels is useful for monitoring lipid synthesis and metabolism.

Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.

Recent Product Citations
  1. Li, R.H. et al. (2021). A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid-liquid phase separation to promote oncogenic YAP signaling. Cell Res. doi: 10.1038/s41422-021-00530-9.
  2. Nadeem, A. et al. (2021). Phosphatidic acid-mediated binding and mammalian cell internalization of the Vibrio cholerae cytotoxin MakA. PLoS Pathog. 17(3):e1009414. doi: 10.1371/journal.ppat.1009414.
  3. Zhang, G. et al. (2020). CD4+ T Cell-Mimicking Nanoparticles Broadly Neutralize HIV-1 and Suppress Viral Replication through Autophagy. mBio. 11(5):e00903-20. doi: 10.1128/mBio.00903-20.
  4. Loh, J. et al. (2019). An acute decrease in plasma membrane tension induces macropinocytosis via PLD2 activation. J Cell Sci. 132(17). pii: jcs232579. doi: 10.1242/jcs.232579.
  5. Kumar Sah, R. et al. (2019). Phosphatidic acid homeostasis regulated by a type-2 phosphatidic acid phosphatase represents a novel druggable target in malaria intervention. Cell Death Discov. 5:107. doi: 10.1038/s41420-019-0187-1.
  6. Zhang, P. et al. (2019). Lipin 2/3 phosphatidic acid phosphatases maintain phospholipid homeostasis to regulate chylomicron synthesis. J Clin Invest. 129(1):281-295. doi: 10.1172/JCI122595.
  7. Han, H. et al. (2018). Regulation of the Hippo Pathway by Phosphatidic Acid-Mediated Lipid-Protein Interaction. Mol Cell. 72(2):328-340.e8. doi: 10.1016/j.molcel.2018.08.038.
  8. Lutkewitte, A.J. et al. (2018). Lipin deactivation after acetaminophen overdose causes phosphatidic acid accumulation in liver and plasma in mice and humans and enhances liver regeneration. Food Chem Toxicol. 115:273-283. doi: 10.1016/j.fct.2018.03.014.