Total Phosphatidic Acid Assay Kit

Total Phosphatidic Acid Assay Kit
  • Detects total phosphatidic acid content (PA and LPA) in cell lysate samples
  • Detection sensitivity of approximately 5 µM phosphatidic acid
  • Phosphatidic Acid standard curve included
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Total Phosphatidic Acid Assay Kit
Catalog Number
100 assays
Manual/Data Sheet Download
SDS Download
Product Details

Phosphatidic Acid (PA) is a phospholipid that serves three main roles in the cell: it is a precursor for lipid biosynthesis, provides membrane structure, and acts as a signaling lipid by recruiting cytosolic proteins to the cell membrane. PA concentrations are maintained at low levels in the cell by phospholipid phosphatases, which convert PA to diacylglycerol (DAG). Measurement of cellular PA levels is useful for monitoring lipid synthesis and metabolism.

Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.

Recent Product Citations
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  3. Nath, A.S. et al. (2022). Modulation of the cell membrane lipid milieu by peroxisomal β-oxidation induces Rho1 signaling to trigger inflammatory responses. Cell Rep. 38(9):110433. doi: 10.1016/j.celrep.2022.110433.
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  6. Zhang, G. et al. (2020). CD4+ T Cell-Mimicking Nanoparticles Broadly Neutralize HIV-1 and Suppress Viral Replication through Autophagy. mBio. 11(5):e00903-20. doi: 10.1128/mBio.00903-20.
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  8. Kumar Sah, R. et al. (2019). Phosphatidic acid homeostasis regulated by a type-2 phosphatidic acid phosphatase represents a novel druggable target in malaria intervention. Cell Death Discov. 5:107. doi: 10.1038/s41420-019-0187-1.
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