96-Well Cell Invasion Assay, Basement Membrane

  • Fully quantify cell invasion with no manual cell counting
  • Plate inserts are precoated with ECM gel layer (Basement Membrane)
  • Fluorometric quantitation

 

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CytoSelect™ 96-Well Cell Invasion Assay, Basement Membrane
Catalog Number
CBA-112
Size
96 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$610.00
Product Details

The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis.

Our CytoSelect™ 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Basement Membrane, an ECM protein mix isolated from EHS tumor cells.

CytoSelect™ Cell Invasion Assay Principle. Cell suspensions are placed on top of the gel matrix inside the upper chamber. After 24-48 hours, invasive cells move through the matrix and adhere to the bottom membrane of the insert. Non-invasive cells are then removed from the upper chamber, and invasive cells can be either stained and counted using a light microscope or quantified after extraction using a colorimetric or fluorometric plate reader.

Recent Product Citations
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