96-Well Cell Invasion Assay, Basement Membrane

96-Well Cell Invasion Assay, Basement Membrane
  • Fully quantify cell invasion with no manual cell counting
  • Plate inserts are precoated with ECM gel layer (Basement Membrane)
  • Fluorometric quantitation


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CytoSelect™ 96-Well Cell Invasion Assay, Basement Membrane
Catalog Number
96 assays
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Product Details

The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis.

Our CytoSelect™ 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Basement Membrane, an ECM protein mix isolated from EHS tumor cells.

CytoSelect™ Cell Invasion Assay Principle. Cell suspensions are placed on top of the gel matrix inside the upper chamber. After 24-48 hours, invasive cells move through the matrix and adhere to the bottom membrane of the insert. Non-invasive cells are then removed from the upper chamber, and invasive cells can be either stained and counted using a light microscope or quantified after extraction using a colorimetric or fluorometric plate reader.

Recent Product Citations
  1. Basu, R. et al. (2023). Structure and function of a dual antagonist of the human growth hormone and prolactin receptors with site-specific PEG conjugates. J Biol Chem. 299(8):105030. doi: 10.1016/j.jbc.2023.105030.
  2. Han, D.H. et al. (2023). Chronic Exposure to TDI Induces Cell Migration and Invasion via TGF-β1 Signal Transduction. Int J Mol Sci. 24(7):6157. doi: 10.3390/ijms24076157.
  3. Arikoglu, H. et al. (2022). The effects of Juglone-Selenium combination on invasion and metastasis in pancreatic cancer cell lines. Afri Health Sci. 22(2): 334-342. doi: 10.4314/ahs.v22i2.37.
  4. Nagase, Y. et al. (2022). Anti-lipolysis-stimulated lipoprotein receptor monoclonal antibody as a novel therapeutic agent for endometrial cancer. BMC Cancer. 22(1):679. doi: 10.1186/s12885-022-09789-6.
  5. Nii, T. & Tabata, Y. et al. (2021). Immunosuppressive mesenchymal stem cells aggregates incorporating hydrogel microspheres promote an in vitro invasion of cancer cells. Regen Ther. doi: 10.1016/j.reth.2021.11.006.
  6. Ikeda, J. et al. (2021). COMMD5 Inhibits Malignant Behavior of Renal Cancer Cells. Anticancer Res. 41(6):2805-2815. doi: 10.21873/anticanres.15061.
  7. Nii, T. et al. (2020). A co-culture model of 3D TAM and 3D CAF combined with biomolecules release for cancer cell migration. Tissue Eng Part A. doi: 10.1089/ten.TEA.2020.0095.
  8. Nii, T. et al. (2020). A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system. Regen Ther. 14:196-204. doi: 10.1016/j.reth.2020.02.003.
  9. Tanaka, E. et al. (2019). Expression of circular RNA CDR1‑AS in colon cancer cells increases cell surface PD‑L1 protein levels. Oncol Rep. doi: 10.3892/or.2019.7244.
  10. Pereira, M.C. et al. (2019). In Vitro Analysis of the Combinatory Effects of Novel Aminonaphthoquinone Derivatives and Curcumin on Breast Cancer Progression. Anticancer Res. 40(1):229-238. doi: 10.21873/anticanres.13944.
  11. Kowolik, C.M. et al. (2019). Attenuation of hedgehog/GLI signaling by NT1721 extends survival in pancreatic cancer. J Exp Clin Cancer Res. 38(1):431. doi: 10.1186/s13046-019-1445-z.
  12. Tanaka, Y. et al. (2019). Differential Prognostic Relevance of Promoter DNA Methylation of CDO1 and HOPX in Primary Breast Cancer. Anticancer Res. 39(5):2289-2298. doi: 10.21873/anticanres.13345.
  13. Ishii, S. et al. (2017). The H19-PEG10/IGF2BP3 axis promotes gastric cancer progression in patients with high lymph node ratios. Oncotarget. 8(43):74567-74581. doi: 10.18632/oncotarget.20209.
  14. Chueca, E. et al. (2016). Proton pump inhibitors display antitumor effects in Barrett's Adenocarcinoma cells. Front. Pharmacol. 7:452.
  15. Zhou, Z.L. et al. (2016). Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment. Int. J. Nanomedicine 11:6533-6545.
  16. Smith, R.W. et al. (2016). Therapeutic potential of GW501516 and the role of Peroxisome proliferator-activated receptor ß/d and B-cell lymphoma 6 in inflammatory signaling in human pancreatic cancer cells. Biochem. Biophys. 8:395-402.
  17. Saha, S. K. et al. (2016). KRT19 directly interacts with β-catenin/RAC1 complex to regulate NUMB-dependent NOTCH signaling pathway and breast cancer properties. Oncogene. doi:10.1038/onc.2016.221.
  18. Hirahata, M. et al. (2016). PAI-1, a target gene of miR-143, regulates invasion and metastasis by upregulating MMP-13 expression of human osteosarcoma. Cancer Med. doi:10.1002/cam4.651.
  19. Knappe, N. et al. (2016). Directed de-differentiation using partial reprogramming induces invasive phenotype in melanoma cells. Stem Cells.doi:10.1002/stem.2284.
  20. Adam, M. G. et al. (2015). SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status. Cell Cycle. 14:3734-3747.
  21. Yamamoto, K. et al. (2014). miR-379/411 cluster regulates IL-18 and contributes to drug resistance in malignant pleural mesotheliomaOncol Rep. 32:2365-2372.
  22. Takeuchi, S. et al. (2014). Significance of osteopontin in the sensitivity of malignant pleural mesothelioma to pemetrexedInt J Oncol. 44:1886-1894.
  23. Ichijo, S. et al. (2014). Activation of the RhoB signaling pathway by thyroid hormone receptor β in thyroid cancer cells. PLoS One. 9:e116252.
  24. Ruan, M. et al. (2014). Activation of Toll-like receptor-9 promotes cellular migration via up-regulating MMP-2 expression in oral squamous cell carcinoma. PLoS One. 9:e92748.
  25. Ismail, I. A. et al. (2014). DJ-1 upregulates breast cancer cell invasion by repressing KLF17 expression. Br J Cancer. 110:1298-1306.
  26. Leung, W.H. et al. (2013). Modulation of NKG2D Ligand Expression and Metastasis in Tumors by Spironolactone via RXR{gamma} Activation. J. Exp. Med. 210:2675-2692.
  27. Nakayama, K. et al. (2013). cAMP-response Element-binding Protein (CREB) and NF-{kappa}B Transcription Factors Are Activated during Prolonged Hypoxia and Cooperatively Regulate the Induction of Matrix Metalloproteinase MMP1. J. Biol. Chem. 288:22584-22595.
  28. Chen, Z. et al. (2012). The Iron Chelators Dp44mT and DFO Inhibit TGF-β-induced Epithelial-Mesenchymal Transition via Up-Regulation of N-Myc Downstream-regulated Gene 1 (NDRG1). J.Biol.Chem. 287:17016-17028.
  29. Beach, J.R. et al. (2011). Myosin II Isoform Switching Mediates Invasiveness after TGF-ß-Induced Epithelial-Mesenchymal Transition. PNAS 108:17991-17996.
  30. Eckstein, N. et al. (2009). Hyperactivation of the Insulin-like Growth Factor Receptor I Signaling Pathway is an Essential Event for Cisplatin Resistance of Ovarian Cancer Cells. Cancer Res. 69:2996-3003.