24-Well Chemotaxis / Cell Invasion Assay Combo Kits

24-Well Chemotaxis / Cell Invasion Assay Combo Kits
  • Fully quantify chemotaxis and cell invasion with no manual cell counting
  • Includes two plates with 8 µm membrane inserts: one uncoated for chemotaxis and one precoated on top of the membrane with ECM matrix (basement membrane) for cell invasion
  • Colorimetric or fluorometric quantitation

 

Frequently Asked Questions about Chemotaxis Assays

Frequently Asked Questions about Cell Invasion Assays

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CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm
Catalog Number
CBA-100-C
Size
12 migration assays + 12 invasion assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$825.00
CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm
Catalog Number
CBA-101-C
Size
12 migration assays + 12 invasion assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$825.00
CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm
Catalog Number
CBA-100-C-5
Size
5x12 migration assays + 5x12 invasion assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$3,520.00
Product Details

If you are assaying both invasive and migratory properties of your cells, order one of our economic CytoSelect™ Cell Migration  / Invasion Assay Combo kits. These kits save you money compared to buying separate chemotaxis and cell invasion kits.

Each 24-well combo kit provides sufficient reagents to perform 12 cell migration plus 12 cell invasion assays.

Migration of Human Fibrosarcoma HT-1080 Cells.Cells were seeded at 30,000 cells per well of a 24-well plate and allowed to migrate toward 10% FBS for 4 hours in either the presence or absence of 2µM Cytochalasin D. Migratory cells on the bottom of the polycarbonate membrane were stained (top) or quantified in a fluorescence plate reader (bottom).

Effects of Cytochalasin D on Invading Cells using the CytoSelect™ 24-Well Cell Invasion Assay. HT-1080 and NIH3T3 cells (negative control) were seeded at 300,000 cells/well and allowed to invade toward 10% FBS for 24 hrs in the presence or absence of 2µM Cytochalasin D. Invasive cells, on the bottom of the invasion membrane, were stained (above) and then quantified at OD 560 nm after extraction using a standard plate reader (not shown).

Recent Product Citations
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  2. Cassandri, M. et al. (2020). ZNF750 represses breast cancer invasion via epigenetic control of prometastatic genes. Oncogene. doi: 10.1038/s41388-020-1277-5 (#CBA-100-C).
  3. He, A. et al. (2020). CDKL3 promotes osteosarcoma progression by activating Akt/PKB. Life Sci Alliance. 3(5). pii: e202000648. doi: 10.26508/lsa.202000648 (#CBA-100-C).
  4. Qiao, B. et al. (2020). miR-205-3p Functions as a Tumor Suppressor in Ovarian Carcinoma. Reprod. Sci. doi: 10.1007/s43032-019-00047-y (#CBA-100-C).
  5. Kisin, E. R. et al. (2020). Enhanced morphological transformation of human lung epithelial cells by continuous exposure to cellulose nanocrystals. Chemosphere. doi: 10.1016/j.chemosphere.2020.126170 (#CBA-100-C).
  6. Hara, T. et al. (2020). Cabozantinib inhibits AXL- and MET-dependent cancer cell migration induced by growth-arrest-specific 6 and hepatocyte growth factor. Biochem Biophys Rep. doi: 10.1016/j.bbrep.2020.100726 (#CBA-101-C).
  7. Li, K. et al. (2019). Discovering novel P38α inhibitors for the treatment of prostate cancer through virtual screening methods. Future Med Chem. 11(24):3125-3137. doi: 10.4155/fmc-2019-0223 (#CBA-100-C).
  8. Cai, F. et al. (2019). MiR-539 inhibited the malignant behaviors of breast cancer cells by targeting SP1. Biochem Cell Biol. doi: 10.1139/bcb-2019-0111 (#CBA-100-C).
  9. Shirasu, N. et al. (2019). Highly versatile cancer photoimmunotherapy using photosensitizer-conjugated avidin and biotin-conjugated targeting antibodies. Cancer Cell Int. doi: 10.1186/s12935-019-1034-4 (#CBA-100-C).
  10. Fezza, M. et al. (2019). DKK1 promotes hepatocellular carcinoma inflammation, migration and invasion: Implication of TGF-β1. PLoS One. 14(9):e0223252. doi: 10.1371/journal.pone.0223252 (#CBA-100-C).
  11. Ding, L. et al. (2019). Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ. Nat Commun. 10(1):4182. doi: 10.1038/s41467-019-12125-5 (#CBA-100-C).
  12. Lee, Y.J. et al. (2019). Pifithrin-μ induces necroptosis through oxidative mitochondrial damage but accompanies epithelial-mesenchymal transition-like phenomenon in malignant mesothelioma cells under lactic acidosis. Arch Pharm Res. doi: 10.1007/s12272-019-01181-6 (#CBA-100-C).
  13. Piazzi, M. et al. (2019). Expression of the double-stranded RNA-dependent kinase PKR influences osteosarcoma attachment independent growth, migration, and invasion. J Cell Physiol. doi: 10.1002/jcp.29024 (#CBA-100-C).
  14. Oria, V.O. et al. (2019). ADAM9 contributes to vascular invasion in pancreatic ductal adenocarcinoma. Mol Oncol. 13(2):456-479. doi: 10.1002/1878-0261.12426 (#CBA-100-C).
  15. Joo, L.J.S. et al. (2019). RET kinase-regulated microRNA-153-3p improves therapeutic efficacy in medullary thyroid carcinoma. Thyroid. doi: 10.1089/thy.2018.0525 (#CBA-100-C).
  16. Seo, S. et al. (2019). The GTP binding activity of transglutaminase 2 promotes bone metastasis of breast cancer cells by downregulating microRNA-205. Am J Cancer Res. 9(3):597-607 (#CBA-100-C).
  17. Kosibaty, Z. et al. (2019). Cytoplasmic expression of epithelial cell transforming sequence 2 in lung adenocarcinoma and its implications for malignant progression. Lab Invest. 99(4):551-567. doi: 10.1038/s41374-018-0142-4 (#CBA-100-C).
  18. Chosokabe, M. et al. (2019). SIRT1 Expression Is Associated With Cell Proliferation in Angiosarcoma. Anticancer Res. 39(3):1143-1150. doi: 10.21873/anticanres.13223 (#CBA-100-C).
  19. Estekizadeh, A. et al. (2019). 5‑Azacytidine treatment results in nuclear exclusion of DNA methyltransferase‑1, as well as reduced proliferation and invasion in human cytomegalovirus‑infected glioblastoma cells. Oncol Rep. 41(5):2927-2936. doi: 10.3892/or2019.7074 (#CBA-100-C).
  20. D'Almeida, O. et al. (2019). Encapsulated miR-200c and Nkx2.1 in a nuclear/mitochondria transcriptional regulatory network of non-metastatic and metastatic lung cancer cells. BMC Cancer. 19(1):136. doi: 10.1186/s12885-019-5337-6 (#CBA-101-C).
  21. Li, S. et al. (2018). Fos-like antigen 2 (FOSL2) promotes metastasis in colon cancer. Exp Cell Res. 373(1-2):57-61. doi: 10.1016/j.yexcr.2018.08.016 (#CBA-100-C).
  22. Parada, C.A. et al. (2018). Kinome and phosphoproteome of high-grade meningiomas reveal AKAP12 as a central regulator of aggressiveness and its possible role in progression. Sci Rep. 8(1):2098. doi: 10.1038/s41598-018-19308-y (#CBA-100-C).
  23. Kathuria, H. et al. (2018). NKX2-1-AS1 negatively regulates CD274/PD-L1, cell-cell interaction genes, and limits human lung carcinoma cell migration. Sci Rep. 8(1):14418. doi: 10.1038/s41598-018-32793-5 (#CBA-101-C).
  24. She, S. et al. (2018). Proteomics Based Identification of Autotaxin As An Anti-Hepatitis B Virus Factor and a Promoter of Hepatoma Cell Invasion and Migration. Cell Physiol Biochem. 45(2):744-760. doi: 10.1159/000487166 (#CBA-101-C).
  25. Yamauchi, K. et al. (2017). 3,4’,7-O-trimethylquercetin inhibits invasion and migration of ovarian cancer cells. Anticancer Res. 37(6):2823-2829 (#CBA-101-C).
  26. Bhansali, M. et al. (2015). TM4SF3 and AR: A nuclear complex that stabilizes both proteins. Mol Endocrinol. doi:10.1210/me.2015-1075 (#CBA-101-C).
  27. Lombard, A. P. et al. (2015). Dicer ablation promotes a mesenchymal and invasive phenotype in bladder cancer cells. Oncol Rep. 34:1526-1532 (#CBA-101-C).
  28. Saini, S. et al. (2014). Regulation of SRC Kinases by microRNA-3607 Located in a Frequently Deleted Locus in Prostate Cancer. Mol Cancer Ther. 13:1952-1963 (#CBA-101-C).
  29. Gobeil, S. et al. (2008). A Genome-Wide shRNA Screen Identifies GAS1 as a Novel Melanoma Metastasis Suppressor Gene. Genes Dev. 22(21):2932-2940 (#CBA-101-C).
  30. Uddin, M. et al. (2008). Marinobufagenin Inhibits Proliferation and Migration of Cytotrophoblast and CHO cells. Placenta. 29(3):266-273 (#CBA-101-C).