Our Chemotaxis Assays use a Boyden Chamber containing a membrane with a defined pore size. Cells are seeded above the membrane and are allowed to migrate toward a chemoattractant in the lower chamber.
For optimal results the membrane pore size should be somewhat smaller than the size of the cell to be assayed. Use the following table to select the right pore size for your migration experiments.
Chemotaxis Assay Selection by Cell Type
|Cell Type||Pore Size||Cell Type||Pore Size|
|Astrocytes||12 µm||Lymphocytes||5 µm|
|Cancer cell lines||8 µm||Macrophages||5 µm|
|Endothelial cells||8 µm||Monocytes||5 µm|
|Epithelial cells||8 µm||Neutrophils||3 µm|
|Fibroblasts||8 µm||Slow-moving cells||12 µm|
|Leukocytes||3 µm||Tumor cells||8 µm|